Abstract
A simple and rapid technique is reported for the preliminary screening of fungi-toxic extracts/samples by direct spotting onto silica gel plates and subsequent over-spraying with a fungal spore suspension. After incubation fungi-toxicity is indicated by a growth inhibition zone, the area of which is related to the concentration of the sample.
Article PDF
Avoid common mistakes on your manuscript.
References
FromtlingR.A. & BulmerG.S. 1978 In vitro effect of aqueous extract of garlic (Allium sativum) on the growth and viability of Cryptococcus neoformans. Mycologia 70, 397–405.
GarberR.H. & HoustonB.R. 1959 An inhibitor of Verticillium albo-atrum in cotton seed. Phytopathology 49, 449–450.
HomansA.L. & FuchsA. 1970 Direct bioautography on thinlayer chromatograms as a method for detecting fungitoxic substances. Journal of chromatography 51, 327–329.
JanssenA.M., SchefferJ.J.C. & SvendsenA.B. 1987 Anti-microbial activity of essential oils: a 1976–1986 literature review. Aspects of the test methods. Planta Medica 53, 395–398.
LudwigA. & BollerT. 1990 A method for the study of fungal growth inhibition by plant proteins. FEMS Microbiology Letters 69, 61–66.
PaxtonJ.D. 1991 Assays for antifungal activity. Methods in Plant Biochemistry 6, 33–46.
PeroR.W. & OwensR.G. 1971 Simple micromethod for detecting antifungal activity. Applied Microbiology 21, 546–547.
Additional information
B.K. Rana and V. Taneja are with the Department of Biochemistry. Faculty of Science, Banaras Hindu University, Varanasi 221005, India; U.P. Singh is with the Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India.
Rights and permissions
About this article
Cite this article
Rana, B.K., Singh, U.P. & Taneja, V. A rapid method for detecting fungi-toxic substances. World Journal of Microbiology & Biotechnology 12, 301–302 (1996). https://doi.org/10.1007/BF00360934
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00360934