Summary
Rates of protein synthesis and oxygen consumption (\(\dot M\)O2) in cod were compared in both fasted and refed animals. During a 14-day fast both protein synthesis and respiration rates fell to stable values after 6 days. When a meal of whole sandeel at 6% body weight was fed to fish fasted for 6 days, protein synthesis and (\(\dot M\)O2) increased to a maximum at between 12 and 18 h after feeding. Peak (\(\dot M\)O2) was about twice the pre-feeding values, while whole animal protein synthesis increased four-fold. There were differences between tissues in the timing of maximum protein synthesis; the liver and stomach responded faster than the remainder of the body. Maximum protein synthesis rates in the liver and stomach occurred at 6 h after feeding, at which time their calculated contribution to total (\(\dot M\)O2) was 11%. Similar calculations suggested that the integrated increment in whole animal protein synthesis contributed between 23% and 44% of the post-prandial increase in (\(\dot M\)O2). It was concluded that protein synthesis is an important contributor to increased (\(\dot M\)O2) after feeding in cod.
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Abbreviations
- A s :
-
absolute rate of protein synthesis
- ASDA:
-
apparent specific dynamic action
- ATP:
-
adenosine triphosphate
- k s :
-
fractional rate of protein synthesis
- k s/RNA:
-
amount of protein synthesized per unit RNA
- (\(\dot M\)O2):
-
oxygen consumption
- PCA:
-
perchloric acid
- RNA:
-
ribonucleic acid
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Lyndon, A.R., Houlihan, D.F. & Hall, S.J. The effect of short-term fasting and a single meal on protein synthesis and oxygen consumption in cod, Gadus morhua . J Comp Physiol B 162, 209–215 (1992). https://doi.org/10.1007/BF00357525
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DOI: https://doi.org/10.1007/BF00357525