Abstract
A survey of intraspecific allozymic variation among samples of the damselfishes Chrysiptera cyanea and Pomacentrus coelestis collected from Palau and Okinawa revealed levels of genetic divergence far in excess of estimates reported previously for populations of coral reef fishes. Absolute or nearly fixed differences in allele frequencies were detected at ADA *, sMDH-2 *, MEP-1 *, PEPB *, PEP-LT * and sSOD *, and at sAAT *, GPI-A *, LDH-1 *, and PEPB *, among the geographic samples of C. cyanea and P. coelestis, respectively. Examination of allele frequencies at most other loci (26 for C. cyanea; 24 for P. coelestis) revealed slight differentiation or identical fixation among the geographic samples. The observed patterns of allele frequency distribution suggest the existence of localized demes of these fishes: these demes may be cryptic and/or incipient species. Whether or not speciation is incipient, the observed patterns of allozymic and isozymic variability suggest that natural selection is a factor in the maintenance of population substructuring of the study species. Pronounced allelic differences were highly concentrated at a small number of loci: strongly bimodal frequency distributions of loci relative to their genetic identities (I) were observed for among-population comparisons in both study species. Allozymes encoded by the PEPB * locus in samples of the noncongeners from Palau exhibited identical electrophoretic mobilities. In Palauan P. coelestis, mSOD * is expressed but sSOD * apparently is not, whereas in Okinawan P. coelestis, both mSOD * and sSOD * are expressed.
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References
Allen, G. R. (1975). Damselfishes of the South Seas. T. F. H. Publications, Inc., Neptune City, New Jersey
Allen, G. R. (1991). Damselfishes of the World. Mergus Publishers, Melle, Germany
Ayala, F. J. (1974). Biological evolution: natural selection or random walk? Am. Scient. 62: 692–701
Bell, L. J., Moyer, J. T., Numachi, K. (1982). Morphological and genetic variation in Japanese populations of the anemonefish Amphiprion clarkii. Mar. Biol. 72: 99–108
Ehrlich, P. R. (1975). The population biology of coral reef fishes. A. Rev. Ecol. Syst. 6: 211–247
Gorman, G. C., Kim, Y. J. (1977). Genotypic evolution in the face of phenotypic conservativeness: Abudefduf (Pomacentridae) from the Atlantic and Pacific sides of Panama. Copeia 1977 (4): 694–697
Graves, J. E., Rosenblatt, R. H., Somero, G. N. (1983). Kinetic and electrophoretic differentiation of lactate dehydrogenase of teleost species-pairs from the Atlantic and Pacific coasts of Panama. Evolution 37: 30–37
Hillis, D. M. (1984). Misuse and modification of Nei's genetic distance. Syst. Zool. 33: 238–240
Koehn, R. K. (1978). Physiology and biochemistry of enzyme variation: the interface of ecology and population genetics. In: Brussard, P. F. (ed.) Ecological genetics: the interface. Springer, Heidelberg, New York, p. 51–72
Koehn, R. K., Milkman, R., Mitton, J. B. (1976). Population genetics of marine pelecypods: selection, migration, and genetic differentiation in the blue mussel Mytilus edulis. Evolution 35: 2–32
Koehn, R. K., Newell, R. I. E., Immerman, F. (1980). Maintenance of an aminopeptidase allele frequency cline by natural selection. Proc. natn. Acad. Sci. U.S.A. 77: 5385–5389
Koehn, R. K., Zera, A. J., Hall, J. G. (1983). Enzyme polymorphism and natural selection. In: Nei, M, Koehn R. K., (eds.) Evolution of genes and proteins. Sianuer Associates, Sunderland, Massachusetts, p. 115–136
Lacson, J. M., Bassler, C. P. (1992). Biochemical systematics of fishes of the genus Stegastes (Pomacentridae) from the Southern Marianas. Mar. Biol. 113: 457–462
Levene, H. (1949). On a matching problem arising in genetics. Ann. math. Statist. 20: 91–94
Masuda, H., Amaoka, K., Araga, C., Uyeno, T., Yoshino, T. (1984). The fishes of the Japanese Archipelago. Tokai University Press, Tokyo
Morizot, D. C., Schmidt, M. E. (1990). Starch gel electrophoresis and histochemical visualization of proteins. In: Whitmore, D. H. (ed.) Electrophoretic and isoelectric focusing techniques in fisheries management. CRC Press, Boca Raton, Florida
Myers, R. F. (1989). Micronesian reef fishes: a practical guide to the identification of the coral reef fishes of the tropical central and western Pacific. 2nd ed. Coral Graphics, Guam, USA
Nomenclature Committee of the International Union of Biochemistry (1984). Enzyme nomenclature: recommendations of the nomenclature committee of the international union of biochemistry on the nomenclature and classification of enzyme-catalyzed reactions. Academic Press, San Diego, California
Robertson, D. R. (1973). Field observations on the reproductive behavior of a pomacentrid fish, Acanthochromis polyacanthus. Z. Tierpsychol. 32: 319–324
Shaklee, J. B. (1984). Genetic variation and population structure in the damselfish, Stegastes fasciolatus, throughout the Hawaiian archipelago. Copeia 1984 (3): 629–640
Shaklee, J. B., Allendorf, F. W., Morizot, D. C., Whitt, G. S. (1990). Gene nomenclature for protein-coding loci in fish. Trans. Am. Fish. Soc. 119: 2–15
Shaklee, J. B., Tamaru, C. S. (1981). Biochemical and morphological evolution of Hawaiian bonefishes (Albula). Syst. Zool. 30: 125–146
Shaklee, J. B., Tamaru, C. S., Waples, R. S. (1982). Speciation and evolution of marine fishes studied by the electrophoretic analysis of proteins. Pacif. Sci. 36: 141–157
Smith, P. J., Robertson, D. A. (1981). Genetic evidence for two species of sprat (Sprattus) in New Zealand waters. Mar. Biol. 62: 227–233
Sokal, R. R., Rohlf, F. J. (1969). Biometry. The principles and practice of statistics in biological research. W. H. Freeman & Co., San Francisco
Swofford, D. L., Selander, R. B. (1981). BIOSYS-1: a FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics. J. Hered. 72: 281–283
Thresher, R. E., Colin, P. L., Bell, L. J. (1989). Planktonic duration, distribution and population structure of western and central Pacific Damselfishes (Pomacentridae). Copeia 1989 (2): 420–434
Vawter, A. T., Rosenblatt, R., Gorman, G. C. (1980). Genetic divergence among fishes of the eastern Pacific and the Caribbean: support for the molecular clock. Evolution 34: 705–711
Vrijenhoek, R. C., Pfeiler, E., Wetherington, J. D. (1992). Balancing selection in a desert stream-dwelling fish, Poeciliopsis monacha. Evolution 46: 1642–1657
Waples, R. S. (1981). A biochemical and morphological review of the lizardfish genus Saurida in Hawaii, with the description of a new species. Pacif. Sci. 35: 217–235
Wright, S. (1978). Evolution and the genetics of populations: variability within and among natural populations. University of Chicago Press, Chicago
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Communicated by M. G. Hadfield, Honolulu
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Lacson, J.M. Fixed allele frequency differences among Palauan and Okinawan populations of the damselfishes Chrysiptera cyanea and Pomacentrus coelestis . Marine Biology 118, 359–365 (1994). https://doi.org/10.1007/BF00350292
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DOI: https://doi.org/10.1007/BF00350292