Abstract
Paralytic shellfish toxin profiles of the dinoflagellate Gymnodinium catenatum Graham were investigated as a possible biochemical marker to distinguish different geographic populations of this species. Isolates obtained between 1986 and 1988 from Japan, Tasmania (Australia) and Galicia (Spain) were cultured under similar conditions and the toxins produced were analyzed using HPLC. Variations in temperature, salinity, and nitrate and phosphate levels in the culture medium had no significant effect on the toxin profile, suggesting that toxins can be used as a stable biochemical marker for this dinoflagellate. All the isolates produced mainly toxins of the N-sulfocarbamoyl group (C1–C4, gonyautoxins 5 and 6) but their relative abundance differed according to their geographic origin. Furthermore, only the Australian population produced the newly found 13-deoxydecarbamoyl toxins, and these could readily be used to distinguish the Australian populations from those of the other two countries.
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Anderson, D. M., Sullivan, J. J., Reguera, B. (1989). Paralytic shell-fish poisoning in Northwest Spain: the toxicity of the dinoflagellate Gymnodinium catenatum. Toxicon 27: 665–674
Anderson, D. M., Kulis, D. M., Sullivan, J. J., Hall, S. (1990). Toxin composition variations in one isolate of the dinoflagellate Alexandrium fundyense. Toxicon 28: 885–893
Balech, E. (1964). El plancton del Mar del Plata durante el period 1961–1962 (Buenos Aires, Argentina). Boln Inst. Biol. Mar. Mar del Plata 4: 1–49
Blackburn, S. I., Hallegraeff, G. M., Bolch, C. J. (1989). Vegetative production and sexual life cycle of the toxic dinoflagellate Gymnodinium catenatum from Tasmania, Australia. J. Phycol. 25: 577–590
Boczar, B. A., Beitler, M. A., Liston, J., Sullivan, J. J., Cattolico, R. A. (1988). Paralytic shellfish toxins in Protogonyaulax tamarensis and Protogonyaulax catenella in axenic culture. Pl. Physiol. 88: 1285–1290
Boyer, G. L., Sullivan, J. J., Anderson, R. J., Taylor, F. J. R., Harison, P. J., Cembella, A. D. (1986). Use of high-performance liquid chromatography to investigate the production of paralytic shellfish toxins by Protogonyaulax spp. in culture. Mar. Biol. 93: 361–369
Carrada, G. C., Casotti, R., Modigh, M., Saggiomo, V. (1991). Presence of Gymnodinium catenatum (Dinophyceae) in a coastal Mediterranean lagoon. J. Plankton Res. 13: 229–230
Cembella, A. D., Sullivan, J. J., Boyer, G. L., Taylor, F. J. R., Andersen, R. J. (1987). Variation in paralytic shellfish toxin composition within the Protogonyaulax tamarensis/catenella species complex; red tide dinoflagellates. Biochem. Syst. Ecol. 15: 171–186
Estrada, M., Sanchez, F. J., Fraga, S. (1984). Gymnodinium catenatum (Graham) en las rias gallegas (NO de Espana). Investigación pesq. 48: 31–40
Fraga, S., Sanchez, F. J. (1985). Toxic and potentially toxic dinoflagellates found in Galician rias (NW Spain). In: Anderson, D. M., White, A. W., Baden, D. (eds.) Toxic dinoflagellates. Elsevier North Holland, New York, p. 51–54
Franca, S., Almeida, J. F. (1989). Paralytic shellfish poisons in bivalve molluscs on the Portuguese coast caused by a bloom of the dinoflagellate Gymnodinium catenatum. In: Okaichi, T., Anderson, D. M., Nemoto, T. (eds.) Red tides: biology, environmental science and toxicology. Elsevier, New York, p. 93–96
Graham, H. W. (1943). Gymnodinium catenatum, a new dinoflagellate from the Gulf of California. Trans. Am. microsc. Soc. 62: 259–261
Hall, S., Strichartz, G., Moczydlowski, E., Ravindran, A., Reichardt, P. B. (1990). The saxitoxins: sources, chemistry, and pharmacology. In: Hall, S., Strichartz, G. (eds.) Marine toxins-origin, structure and molecular pharmacology. American Chemical Society, Washington, D.C., p. 29–65
Hallegraeff, G. M., Bolch, C. J. (1991). Transport of toxic dinoflagellate cysts via ship's ballast water. Mar. Pollut. Bull. 22: 27–30
Hallegraeff, G. M., Bolch, C. J. (1992). Transport of diatom and dinoflagellate resting spores in ship's ballast water: implications for plankton biogeography and aquaculture. J. Plankton Res. 14: 1067–1084
Hallegraeff, G. M., Bolch, C. J., Bryan, J., Koerbin, B. (1990). Microalgal spores in ship's ballast water. A danger to aquaculture. In: Grane'li, E., Sundstrom, B., Edler, L., Anderson, D. M. (eds.) Toxic marine phytoplankton. Elsevier Science Publishing, New York, p. 475–480
Hallegraeff, G. M., Stanley, S. O., Bolch, J. C., Blackburn, S. I. (1989). Gymnodinium catenatum blooms and shellfish toxicity in Southern Tasmania, Australia. In: Okaichi, T., Anderson, D. M., Nemoto, T. (eds.) Red tides: biology, environmental science and toxicology. Elsevier, New York, p. 77–80
Hallegraeff, G. M., Steffensen, D. A., Wetherbee, R. (1988). Three estuarine Australian dinoflagellates that can produce paralytic shellfish toxins. J. Plankton Res. 10: 533–541
Ikeda, T., Matsuno, S., Sato, S., Ogata, T., Kodama, M., Fukuyo, Y., Takayama, M. (1989). First report on paralytic shellfish poisoning caused by Gymnodinium catenatum Graham in Japan. In: Okaichi, T., Anderson, D. M., Nemoto, T. (eds.) Red tides: biology, environmental science and toxicology. Elsevier, New York, p. 411–414
La Barbera Sanchez, A. (1991). Mollusc toxicity caused by a red tide of Gymnodinium catenatum. Red Tide Newsl. 4 (2/3): 1–2
Loeblich, A. R. (1975). A seawater medium for dinoflagellates and the nutrition of Cachonina niei. J. Phycol. 11: 80–86
Mee, L. D., Espinosa, M., Diaz, G. (1986). Paralytic shellfish poisoning with a Gymnodinium catenatum red tide on the Pacific coast of Mexico. Mar. envirl. Res 19: 77–92
Ogata, T., Ishimaru, T., Kodama, M. (1987). Effect of water temperature and light intensity on growth rate and toxicity change in Protogonyaulax tamarensis. Mar. Biol. 95: 217–220
Oshima, Y., Hasegawa, M., Yasumoto, T., Hallegraeff, G. M., Blackburn, S. (1987). Dinoflagellate Gymnodinium catenatum as the source of paralytic shellfish toxins in Tasmanian shellfish. Toxicon 25: 1105–1111
Oshima, Y., Itakura, H., Lee, K. C., Yasumoto, T., Blackburn, S., Hallegraeff, G. (1992). Toxin production by the dinoflagellate Gymnodinium catenatum. In: Smayda, T. J., Shimizu, Y. (eds.) Toxic phytoplankton blooms in the sea. Proceedings of the fifth international conference on toxic marine phytoplankton. Elsevier, Amsterdam (in press)
Oshima, Y., Sugino, K., Itakura, H., Hirota, M., Yasumoto, T. (1990). Comparative studies on paralytic shellfish toxin profile of dinoflagellates and bivalves. In: Grane'li, E., Sundstrom, B., Edler, L., Anderson, D. M. (eds.) Toxic marine phytoplankton. Elsevier Science Publishing, New York, p. 391–396
Oshima, Y., Sugino, K., Yasumoto, T. (1989). Latest advance in HPLC analysis of paralytic shellfish toxins. In: Natori, S., Hashimoto, K., Ueno, Y. (eds.) Mycotoxins and phycotoxins '88. Elsevier Science Publishers, Amsterdam, p. 319–326
Rodriguez-Vazquez, J. A., Oshima, Y., Lee, J. S., Yasumoto, T. (1989). Analysis of toxins in mussels from the Atlantic coast of Spain. In: Natori, S., Hashimoto, H., Ueno, Y. (eds.) Mycotoxins and phycotoxins '88. Elsevier Science Publishers, Amsterdam, p. 367–374
Sullivan, J. J. (1990). High-performance liquid chromatographic method applied paralytic shellfish toxin research. In: Hall, S., Strichartz, G. (eds.) Marine toxin-origin, structure and molecular pharmacology. American Chemical Society, Washington, D.C., p. 66–77
Yuki, K., Yoshimatsu, S. (1987). Morphology of the athecate dinoflagellate Gymnodinium catenatum in culture. Bull. Plankton Soc. Japan 34: 109–117
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Communicated by T. Ikeda, Niigata
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Oshima, Y., Blackburn, S.I. & Hallegraeff, G.M. Comparative study on paralytic shellfish toxin profiles of the dinoflagellate Gymnodinium catenatum from three different countries. Marine Biology 116, 471–476 (1993). https://doi.org/10.1007/BF00350064
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DOI: https://doi.org/10.1007/BF00350064