Abstract
Chlorobenzoic acids (CBA) are frequently dead-end products of partial aerobic biodegradation of polychlorinated biphenyls (PCB). When CBA produced from PCB accumulate in the growth medium, they can inhibit the bacterial growth and consequently, slow down PCB biodegradation. In this study, the effects of seven mono- and dichlorinated CBA on growth of Pseudomonas stutzeri on different substrates and on the PCB degradation by this strain in a liquid mineral medium were tested. 3-CBA was the strongest growth inhibitor for P. stutzeri growing on glucose, benzoate and biphenyl. It was found to inhibit heavily the elimination of some di- and trichlorinated biphenyls. In contrast, its influence on the elimination of more chlorinated congeners was much less significant.
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References
Dercová, K., Baláž, Š., Haluška, L'., Horňák, V., & Holecová, V. 1995 Degradation of PCB by bacteria isolated from long-time contaminated soil. International Journal of Environmental Analytical Chemistry 58, 337–348.
Fava, F., & Marchetti, L. 1991 Degradation and mineralization of 3-chlorobiphenyl by mixed aerobic bacterial culture. Applied Microbiology and Biotechnology 36, 240–245.
Haluška, L'., Baláž, Š., Dercová, K., Benická, E., Krupčík, J., Bielek, P., & Lindišová, G. 1995 Anaerobic degradation of PCB in soils. International Journal of Environmental Analytical Chemistry 58, 327–336.
Krupčík, J., Kočan, A., Petrík, J., Leclerq, P.A., & Ballschmiter, K. 1992 On the use of reference standards for quantitative trace analysis of PCBs by HRGC analyses of technical PCB formulations by HRGC/FID. Chromatographia 33, 514–552.
Pirt, S.J. 1967 A kinetic study of the mode of growth of surface colonies of bacteria and fungi. Journal of General Microbiology 47, 181–197.
Shields, M.S., Hooper, S.W., & Sayler, G.S. 1985 Plasmid-mediated mineralization of 4-chlorobiphenyl. Journal of Bacteriology 168, 882–889.
Sondossi, M., Sylvestre, M., & Ahmad, D. 1992 Effects of chlorobenzoate transformation on the Pseudomons testosteroni biphenyl and chlorobiphenyl degradation pathway. Applied and Environmental Microbiology 58, 485–495.
Vrana, B., Dercová, K., & Baláž, Š. 1995 Monitoring evaporation of polychlorinated biphenyls (PCB) in long term degradation experiments. Biotechnology Techniques 9, 333–338.
Vrana, B., Dercová, K., & Baláž, Š. 1996 Evaporation kinetics of polychlorinated biphenyls during biodegradation experiments. Biotechnology Techniques 10, 37–40.
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The authors are with the Department of Biochemical Technology, Faculty of Chemical Technology, Slovak Technical University, 812 37 Bratislava, Slovakia.
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Vrana, B., Dercová, K., Baláž, Š. et al. Effect of chlorobenzoates on the degradation of polychlorinated biphenyls (PCB) by Pseudomonas stutzeri . World Journal of Microbiology & Biotechnology 12, 323–326 (1996). https://doi.org/10.1007/BF00340208
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DOI: https://doi.org/10.1007/BF00340208