Abstract
Azospirillum brasilense sp7 was exposed to 2mm Zn2+ in minimal medium upon which the cells turned black and non-motile within 24 h. A streptomycin-resistant variant did not exhibit this phenomenon and is sensitized to zinc. A prelude to encystation was the elution of a melanin-like pigment into the medium.
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References
Albrecht, S.L., & Okon, Y. 1980 Cultures of Azospirillum. Methods in Enzymology 69, 740.
Al-Shahwani, M.F., Jazrawi, S.F., Al-Rawl, E.H., & Ayar, N.S. 1984 Growth and heavy metal removal by Klebsiella aerogenes at different pH and temperature. Journal of Environmental Science and Health A19(4), 445–457.
Atlas, R.M. 1993 Handbook of Microbiological Media. Boca Raton, FL: CRC Press Inc.
Baldani, V.L.D., & Dobereiner, J. 1980 Host plant specificity in the infection of cereals with Azospirillum spp. Soil Biology and Biochemistry 12, 433–439.
Berg, R.H., Tyler, M.E., Novick, N.J., Vasil, V., & Vasil, I.K. 1980 Biology of Azospirillum — sugarcane association: enhancement of nitrogenase activity. Applied and Environmental Microbiology 39, 642–649.
Beveridge, T.J. 1989 Metal ions and bacteria. In Metal Ions and Bacteria, eds Beveridge, T.J., & Doyle, R.J. pp. 1–29. New York: John Wiley & Sons.
Bhagat, R., & Srivastava, S. 1994 Effect of zinc on morphology and ultrastructure of Pseudomonas stutzeri RS34. Journal of General and Applied Microbiology 40, 265–270.
Bitton, G., & Freihofer, V. 1978 Influence of extracellular polysaccharides on the toxicity of copper and cadmium toward Klebsiella aerogenes. Microbial Ecology 4, 119–125.
Boddey, R.M., & Dobereiner, J. 1988 Nitrogen fixation associated with grasses and cereals; Recent results and perspectives for future research. Plant and Soil 108, 53–65.
Brown, M.J., & Lester, J.N. 1982 Role of bacterial extracellular polymers in metal uptake in pure bacterial culture and activated sludge. Water Research 16, 1539–1548.
Del Gallo, M., Negi, M., & Neyra, C.A. 1989 Calcofluor and lectin binding exocellular polysaccharides of Azospirillum brasilense and Azospirillum lipoferum. Journal of Bacteriology 171, 3504–3510.
Dobereiner, J., & Baldani, V. 1979 Selective infection of maize roots by streptomycin resistant Azospirillum lipoferum and other bacteria. Canadian Journal of Microbiology 25, 1565–1269.
Eklund, C., Pope, L.H., & Wyss, O. 1966 Relationship of encapsulation and encystment in Azotobacter. Journal of Bacteriology 92, 1828–1830.
Foster, T.J. 1983 Plasmid-determined resistance to antimicrobial drugs and toxic metal ions in bacteria. Microbiological Reviews 47, 361–409.
Geesey, G.G., & Jang, L. 1989 Interactions between metal ions and capsular polymers. In Metal Ions and Bacteria, eds Beveridge, T.J. & Doyle, R.J. pp. 325–356. New York: John Wiley & Sons.
Ivins, B.E., & Holmes, R.K. 1980 Isolation and characterization of melanin-producing (mel) mutants of Vibrio cholerae, Infection and Immunity 27, 721–729.
Jayaraman, J. 1981 Biomolecules I. In Laboratory Manual in Biochemistry, ed Jayaraman, J. pp. 49–60. New Delhi: Wiley Eastern Limited.
Lamm, R.B., & Neyra, C.A. 1981 Characterization and cyst production of asospirilla isolated from selected grasses growing in New Jersey and New York. Canadian Journal of Microbiology 27, 1320–1325.
Lingappa, Y., Sussman, A.S., & Bernstein, I.A. 1963 Effect of light and media upon growth and melanin formation in Aureobasidium pullulans (DeBy) Arn. (= Pullularia pullulans). Mycopathologia et Mycologia Applicata 20, 109–128.
Mangala Gowri, P., & Srivastava, S. 1994 Rich medium based zinc toxicity in Azospirillum brasilense sp7. Indian Journal of Experimental Biology 32, 626–628.
Mangala Gowri, P., & Srivastava, S. 1995 Zinc resistance in Azospirillum brasilense sp7 and its application. In Environmental Biotechnology: Principles and Practice, eds Moo-Young, M., Anderson, W.A. & Chakrabarty, A.M. pp. 135–143. Dordrecht: Kluwer Academic Publishers.
Rai, R., Prasad, V., & Shukla, I.C. 1984 Interaction between finger millet (Eleusine coracana) genotypes and drug-resistant mutants of Azospirillum brasilense in calcareous soil. Journal of Agricultural Science, 102, 521–529.
Sadasivan, L., & Neyra, C.A. 1985 Flocculation in Azospirillum brasilense and Azospirillum lipoferum; exopolysaccharides and cyst formation. Journal of Bacteriology 163, 716–723.
Sadasivan, L., & Neyra, C.A. 1987 Cyst production and brown pigment formation in ageing cultures of Azospirillum brasilense ATCC 29145. Journal of Bacteriology 169, 1670–1677.
Yabuuchi, E., & Ohyama, A. 1972 Characterization of ‘pyomelanin’ producing strains of Pseudomonas aeruginosa. International Journal of Systematic Bacteriology 22, 53–64.
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The authors are with the Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, India
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Gowri, P.M., Srivastava, S. Encapsulation as a response of Azospirillum brasilense sp7 to zinc stress. World Journal of Microbiology & Biotechnology 12, 319–322 (1996). https://doi.org/10.1007/BF00340207
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DOI: https://doi.org/10.1007/BF00340207