Summary
As an initial step towards developing a transposon mutagenesis system in tomato, the maize transposable element Ac was transformed into tomato plants via Agrobacterium tumefaciens. Southern analysis of leaf tissue indicated that in nine out of eleven transgenic plants, Ac excised from the T-DNA and reintegrated into new chromosomal locations. The comparison of Ac banding pattern in different leaves of the same primary transformant provided evidnece for transposition during later stages of transgenic plant development. There was no evidence of Ds mobilization in tomato transformants.
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Yoder, J.I., Palys, J., Alpert, K. et al. Ac transposition in transgenic tomato plants. Mol Gen Genet 213, 291–296 (1988). https://doi.org/10.1007/BF00339594
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DOI: https://doi.org/10.1007/BF00339594