Summary
Several coagulase-negative staphylococci were investigated for their ability to undergo polyethylene glycolinduced protoplast-transformation with plasmid DNA. Among this group of bacteria it was found that S. carnosus TM300 was superior in this respect. Several plasmids isolated from different species were readily transformed with an efficiency ranging between 4×106 to 2×103 transformants per μg plasmid DNA. Since S. carnosus is apathogenic and an important organism in food technology, this organism could be a suitable gram-positive strain for molecular cloning.
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References
Chang S, Cohen SN (1979) High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet 168:111–115
Fischer U, Schleifer KH (1980) Zum Vorkommen der Gram-positiven, katalase-positiven Kokken in Rohwurst. Fleischwirtschaft 60:1046–1051
Götz F, Ahrne S, Lindberg M (1981) Plasmid transfer and genetic recombination by protoplast fusion in staphylococci. J Bacteriol 145:74–81
Götz F, Zabielski J, Philippson L, Lindberg M (1983) DNA homology between the arsenate resistance plasmid pSX267 from Staphylococcus xylosus and the penicillinase plasmid pI258 from Staphylococcus aureus. Plasmid 9: pp
Humphreys GO, Willshaw GA, Anderson ES (1975 A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta 383:457–463
Iordanescu S, Surdeanu M (1976) Two restriction and modification systems in Staphylococcus aureus NCTC 8325. J Gen Microbiol 96:277–281
Iordanescu S, Surdeanu M, Della Latta P, Novick RP (1978) Incompatibility and molecular relationships between small staphylococcal plasmids carrying the same resistance marker. Plasmid 1:468–479
Kreft J, Bernhard K, Goebel W (1978) Recombinant plasmids capable of replication in B. subtilis and E.coli. Mol Gen Genet 162:59–67
Kloos WE, Schleifer KH (1975) Isolation and characterisation of staphylococci from human skin. II. Description of four new species: Staphylococcus warneri, Staphylococcus capitis, Staphylococcus hominis and Staphylococcus simulans. Int J Syst Bacteriol 25:62–79
Lacy RW, Chopra I (1974) Genetic studies of a multi-resistant strain of Staphylococcus aureus. J Med Microbiol 7:285–297
Lindberg M, Novick RP (1973) Plasmid specific transformation in Staphylococcus aureus. J Bacteriol 115:139–145
Niinivaara FP, Pohja MS (1956) Über die Reifung der Rohwurst. I. Mitt.: Die Veränderung der Bakterienflora während der Reifung. Z Lebensm Unters Forsch 104:413–422
Niinivaara FP, Pohja MS (1957a) Über die Reifung der Rohwurst. II. Mitt.: Die Beschreibung der aus Rohwurst isolierten Bakterienstämme und ihre Bedeutung beim Reifungsprozeß. Z Lebensm Unters Forsch 106:187–196
Niinivaara FP, Pohja MS (1957b) Erfahrungen bei der Herstellung von Rohwurst mit Bakterienreinkulturen. Fleischwirtschaft 9:789–790
Novick RP (1967) Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology 33:155–166
Novick RP, Bouanchaud D (1971) Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann NY Acad Sci 182:279–294
Schleifer KH, Fischer U (1982) Description of a new species of the genus Staphylococcus: Staphylococcus carnosus. Int J Syst Bacteriol 32:153–156
Sharp PA, Sugden B, Sambrook J (1973) Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresis. Biochem 12:3055–3063
Wyrick PB, Rogers HJ (1973) Isolation and characterization of cell wall-defective variants of Bacillus subtilis and Bacillus licheniformis. J Bacteriol 116:456–465
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Communicated by A. Böck
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Götz, F., Kreutz, B. & Schleifer, K.H. Protoplast transformation of Staphylococcus carnosus by plasmid DNA. Molec Gen Genet 189, 340–342 (1983). https://doi.org/10.1007/BF00337828
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DOI: https://doi.org/10.1007/BF00337828