Abstract
In paddy fields treated with organic manure or chemical fertilizer or not treated, seasonal changes in soil protease [hydrolysis to benzyloxycarbonyl-l−phenyl-alanyl-l−leucine (z-FLase) or casein (caseinase)] extracted with phosphate buffer were investigated during the cultivation of rice. Both activities reached a maximum on July 21 (after irrigation), and gradually decreased, which was correlated with the soil water content. Increases in z-FLase and caseinase activity extracted coincided with increases in exchangeable NH +4 and the ninhydrin-reactive N content. The overall soil caseinase activity on August 16 (after midsummer drainage) was inhibited by metal chelators and p−chloromercuribenzoic acid and was similar to that extracted with 1.0 mol l−1 phosphate buffer on June 9 (before irrigation), but different from that extracted by 0.1 mol l−1 phosphate buffer on July 22. The overall soil z-FLase activity on August 16 was inhibited by p−chloromercuribenzoic acid and 1,10-phenanthroline, and was similar to that extracted with 0.1 mol l−1 phosphate buffer on June 9, but differed from that extracted on July 22. The results indicate that the soil proteases extracted on July 22 had a high potential for ammonification and were short lived.
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Watanabe, K., Hayano, K. Seasonal variation in extracted proteases and relationship to overall soil protease and exchangeable ammonia in paddy soils. Biol Fertil Soils 21, 89–94 (1996). https://doi.org/10.1007/BF00335998
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DOI: https://doi.org/10.1007/BF00335998