Summary
We report genetic transformation in an intact higher organism, i.e., in xiphophorine fish. The gene to be transferred (Tu) is responsible for the formation of T-melanophores in the platyfish and is involved in the formation of melanomas in platyfish-swordtail hybrids. After injection of Tu-donor DNA into the neural crest region of embryos from Tu-free fish, some of the recipients developed T-melanophores. In a few cases, one or two single T-melanophores were formed during late embryogenesis. In most cases, many T-melanophores developed in young fish and were arranged in several colonies or in a pattern. DNase-degraded Tu-donor DNA, Tu-free fish DNA, as well as DNA from E. coli and adenovirus-2, did not induce T-melanophores. When using DNA from different strains of Tu-donor fish which differed in a regulating gene linked to Tu, the percentages of fish showing T-melanophores paralleled the degree of phenotypic expression of the Tu gene in the DNA donor. The results suggest that the Tu gene has been successfully transferred together with the linked regulating gene.
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Anders A, Anders F, Klinke K (1973a) Regulation of gene expression in the Gordon-Kosswig melanoma system. I. The distribution of the controlling genes in the genome of the xiphophorin fish, Platypoecilus maculatus and Platypoecilus variatus. In: Schröder JH (ed) Genetics and mutagenesis of fish. Springer, Berlin, Heidelberg, New York, pp 33–52
Anders A, Anders F, Klinke K (1973b) Regulation of gene expression in the Gordon-Kosswig melanoma system. II. The arrangement of chromatophore determining loci and regulating elements in the sex chromosomes of Platypoecilus maculatus and Platypoecilus variatus. In: Schröder JH (ed) Genetics and mutagenesis of fish. Springer, Berlin, Heidelberg, New York, pp 53–63
Anders F (1967) Tumor formation in platyfish-swordtail hybrids as a problem of gene regulation. Experientia 23:1–10
Anders F, Anders A, Vielkind U (1974) Regulation of tumor expression in the Gordon-Kosswig melanoma system, and the origin of malignancy. Abstracts of the XIth International Cancer Congress, Florence, 305
Anders F, Diehl H, Scholl E (1980) Differentiation of normal melanophores and neoplastically transformed melanophores in the skin of fishes of genus Xiphophorus. In: Spearman RIC and Riley PA (eds) The skin of vertebrates. Academic Press, London, pp 211–224
Borack LT (1972) Gene action on proliferation and migration in the developing neural crest of black and white axolotls, Ambystoma mexicanum, Shaw. J Exp Zool 179:289–298
Brinster RL (1981) The effect of cells transferred into the mouse blastocyst on subsequent development. J Exp Med 140:1049–1056
Brinster RL, Chen HY, Trumbauer M, Senear AW, Warren R, Palmites RD (1981) Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs. Cell 27:223–231
Brown DD, Gurdon JB (1977) High fidelity transcription of 5S DNA injected into Xenopus oocytes. Proc Natl Acad Sci USA 74:2064–2068
Caspari EW, Friedman HS, Young EM (1975) Genetic changes induced by DNA in Ephestia. Genetics 80:s19-s20
Chen ST, Wahn H, Turner WA, Taylor JD, Chen TT (1974) HSH, cyclic AMP, and melanocyte differentiation. Recent Progr Hormone Res 30:319–345
Diehl HH (1975) Über die Entwicklung des embryonalen Pigmentierungsmusters bei verschiedenen Genotypen von Xiphophorus (Pisces, Poeciliidae). Diplomarbeit, Universität Giessen
Fahmy OG, Fahmy MJ (1965) Genetic properties of exogenous deoxyribonucleic acid at various levels of degradation in Drosophila melanogaster. Nature 207:507–510
Fox AS (1977) Gene transfer in Drosophila melanogaster. In: Rubinstein I (ed) Molecular genetic modification of eukaryotes. Academic Press, New York, pp 101–131
Fox AS, Yoon SB (1970) DNA-induced transformation in Drosophila: locus-specificity and the establishment of transformed stocks. Proc Natl Acad Sci USA 67:1608–1615
Fox AS, Yoon SB, Duggleby WF, Gelbart WM (1971) Genetic transformation in Drosophila. In: Ledoux L (ed) Informative molecules in biological systems. North-Holland Publ Co, Amsterdam, London, pp 313–333
Germeraad S (1976) Genetic transformation in Drosophila by microinjection of DNA. Nature 262:229–231
Gordon H, Gordon M (1957) Maintenance of polymorphism by potentially injurious genes in eight natural populations of the platyfish, Xiphophorus maculatus. J Genet 55:1–44
Gordon JW, Scangos GA, Plotkin DJ, Barbora JA, Ruddle FH (1980) Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci USA 77:7380–7384
Gordon M (1927) The genetics of a viviparous top minnow Platypoecilus; the inheritance of two kinds of melanophores. Genetics 12:253–283
Gordon M (1948) Effects of five primary genes on the sites of melanomas in fishes and the influence of two color genes on their pigmentation. The Biology of Melanomas, NY Acad Sci Spec Publ 4:216–268
Gordon M (1959) The melanoma cell as an incompletely differentiated pigment cell. In: Gordon M (ed) Pigment cell biology. Academic Press, New York, pp 215–239
Gordon M, Lansing L (1943) Cutaneous melanophore eruptions in young fishes during stages preceding melanotic tumor formation. J Morphol 73:231–245
Gradmann-Rebel W, Hemleben V (1976) Incorporation of T4 phage DNA into a specific DNA fraction from the higher plant Matthiola incana. Z Naturforsch 31c:558–564
Haas-Andela H (1976) In vitro-Kultur und Aufzucht von Embryonen lebendgebärender Zahnkarpfen der Gattung Xiphophorus. Zool Anz (Jena) 197:1–5
Humm DG, Young RS (1956) The embryological origin of pigment cells in platyfish-swordtail hybrids. Zoologica (NY) 41:1–10
Illmensee K, Hoppe PC (1981) Nuclear transplantation in Mus musculus: developmental potential of nucleic from implantation embryos. Cell 23:9–18
Jaenisch R, Mintz B (1974) Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA. Proc Natl Acad Sci USA 71:1250–1254
Kallman KD (1975) The platyfish, Xiphophorus maculatus. In: King RC (ed) Handbook of genetics, vol 4. Plenum Press, New York, pp 81–132
Klingmüller W (1976) Genmanipulation und Gentherapie. Springer, Berlin, Heidelberg, New York
Martinovitch PN, Kanazir DT, Knezevitch ZA, Simitch MM (1962) Teratological changes in the offspring of chicken embryos treated with tyrode or with tyrode plus DNA. J Embryol Exp Morphol 10:167–177
Mayer TC (1973) The migratory pathway of neural crest cells into the skin of mouse embryos. Devel Biol 34:39–46
Mintz B, Illmensee K (1975) Normal genetically mosaic mice produced from malignant teratocarcinoma cells. Proc Natl Acad Sci USA 72:3585–3589
Ottolenghi-Nightingale E (1974) DNA-mediated transformation in mammalian cells. In: Cox RD (ed) Cell communication. John Wiley and Sons, New York, pp 233–254
Pellicer A, Robins D, Wold B, Sweet R, Jackson J, Lowy I, Roberts JM, Sim G-K, Silverstein S, Axel R (1980) Altering genotype and phenotype by DNA-mediated gene transfer. Science 209:1414–1422
Pellicer A, Wigler M, Axel R, Silverstein S (1978) The transfer and stable integration of the HSV thymidine kinase gene into mouse cells. Cell 14:133–141
Schmidt ER (1978) Chromatophore development and cell interactions in the skin of xiphophorine fish. Wilhelm Roux's Arch 184:115–134
Schwab M, Vielkind J (1973) A simple effective procedure for the isolation of high molecular weight DNA. IRCS Med Sci (73-3) 3-2-2
Schwab M, Vielkind J, Anders F (1976) An approach to genetic transformation in xiphophorine fish. Mol Gen Genet 144:151–158
Siniscalco M (1972) Correction of genetic defects in cultured mammalian cells. Adv Biosci 8:307–327
Tavolga WN (1949) Embryonic development of the platyfish (Platypoecilus), the swordtail (Xiphophorus) and their hybrids. Bull Am Mus Nat Hist 94:165–229
Turner WA Jr, Taylor JD, Chen TT (1975) Melanosome formation in the goldfish: the role of multivesicular bodies. J Ultrastruct Res 51:16–31
Vielkind J (1971) Versuche zur genetischen Transformation bei lebendgebärenden Zalnkarpfen (Poeciliidae). Applikation heterologer DNA und ihr Schicksal in Rezipienten. Dissertation, Universität Giessen
Vielkind J (1979) Gentransfer mittels exogener DNA bei lebendgebärenden Zahnkarpfen (Poeciliidae). Habilitationsschrift, Universität Giessen
Vielkind J, Girgsdies O (1976) Evidence for uptake of exogenous DNA by fish embryo DNA. IRCS Med Sci 4:268
Vielkind J, Haas-Andela H, Anders F (1976) DNA-mediated transformation in the platyfish-swordtail melanoma system. Experientia 32:1043–1044
Vielkind J, Haas-Andela H, Anders F (1978) Characterization of the genetic factors involved in melanoma formation in xiphophorin fish by transformation experiments. Verh Dtsch Zool Ges Konstanz 1978, p 228
Vielkind J, Haas-Andela H, Anders F (1979) Xiphophorine fish as a carcinogenesis model. X. Transfer of the tumor gene (Tu) and its linked regulating gene (R). ICRDB, Special listing of current cancer research on etiology, epidemiology and biology of melanomas and other skin cancers, No. 146
Vielkind J, Schwab M, Anders F (1973) Fate of bacterial DNA injected into embryos of poeciliid fish. In: Schröder JH (ed) Genetics and mutagenesis of fish. Springer, Berlin, Heidelberg, New York, pp 123–137
Vielkind J, Vielkind U (1982) Melanoma formation in fish of the genus Xiphophorus: a genetically-based disorder in the determination and differentiation of a specific pigment cell. Can J Genet Cytol (in press)
Vielkind J, Vielkind U, Anders F (1971a) Melanotic and amelanotic melanomas in xiphophorin fish. Cancer Res 31:868–875
Vielkind J, Vielkind U, von Grotthuss E, Anders F (1971b) Uptake of bacterial H3-DNA into fish embryos. Experientia 27:347–348
Vielkind U (1976) Genetic control of cell differentiation in platyfish-swordtail melanomas. J Exp Zool 196:197–204
Weston J (1963) A radioautographic analysis of the migration and localization of trunk neural crest cells in the chick. Devel Biol 6:279–310
Wigler M, Pellicer A, Silverstein S, Axel R (1978) Biochemical transfer and stable integration of the HSV thymidine kinase gene into mouse cells. Cell 14:725–731
Wigler M, Sweet R, Sim GK, Wold B, Pellicer A, Lacy E, Maniatis T, Silverstein S, Axel R (1979) Transformation of mammalian cells with genes from procaryotes and eucaryotes. Cell 16:777–785
Willecke K (1980) DNA mediated gene transfer between mammalian cells. In: Celis JE, Graessmann A, Loyter A (eds) Transfer of cell constituents into eukaryotic cells. Plenum Press, New York, London, pp 311–324
Willecke K, Klomfass M, Mireau R, Döhmer J (1979) Intraspecific transfer via total cellular DNA of the gene for hypoxanthine phosphoribosyl transferase into cultured mouse cells. Mol Gen Genet 170:179–185
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Communicated by J. Schell
Part of this work has been done by H.H. for her doctoral thesis
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Vielkind, J., Haas-Andela, H., Vielkind, U. et al. The induction of a specific pigment cell type by total genomic DNA injected into the neural crest region of fish embryos of the genus Xiphophorus . Mol Gen Genet 185, 379–389 (1982). https://doi.org/10.1007/BF00334128
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DOI: https://doi.org/10.1007/BF00334128