Summary
We report that in DNA from one Frankia sp. strain at least some of the nif genes are located on a large indigenous plasmid of 190 kb. Using the cloned nitrogenase structural genes from Klebsiella pneumoniae as hybridization probes, homology was detected with a 5.6 kb EcoRI fragment from the Frankia sp. plasmid. This 5.6 kb fragment was cloned; used as a probe it hybridized to the nif K, D and H genes from Rhizobium meliloti and Klebsiella pneumoniae. The nif probes also hybridized on total DNA blots to a 10 kb EcoRI fragment that is not present on plasmid DNA, suggesting that the nif genes could be located on more than one replicon.
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Simonet, P., Haurat, J., Normand, P. et al. Localization of nif genes on a large plasmid in Frankia sp. strain ULQ0132105009. Molec Gen Genet 204, 492–495 (1986). https://doi.org/10.1007/BF00331030
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DOI: https://doi.org/10.1007/BF00331030