Abstract
Active movements of the chromosomes may be needed in the process, where homologous chromosomes find each other during the meiotic pairing. Because the components of the cytoskeleton are generally believed to be responsible for all movements in living nonmuscle cells, we have analyzed the regulation of the movements of zygotene chromosomes in the male rat by using specific inhibitors of the assembly of the various components of the cytoskeleton. — Colcemid, an inhibitor of microtubule formation, completely inhibited the chromosome movements in vitro at a concentration of 1 μg/ml. This was associated with a damage of the nuclear envelope revealed by the electron microscopic analysis. Another inhibitor of microtubule formation, vinblastine, was ineffective below the level of general toxicity (100 μg/ml). A specific microfilament inhibitor, cytochalasin B was similarly ineffective. — The findings suggest the presence of a specific colcemid-sensitive mechanism in the nuclear envelope of the zygotene spermatocytes, which regulates the movements of the chromosomes during meiotic pairing.
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Bellvé, A.R.: The molecular biology of mammalian spermatogenesis. In: Oxford reviews of reproductive biology, 1 (C.A. Finn, ed.), pp. 159–261. Oxford: Clarendon Press 1979
Bennett, M.D., Smith, J.B.: The effect of colchicine on fibrillar material in wheat meiocytes. J. Cell Sci. 38, 33–47 (1979)
Esponda, P., Giménez-Martin, G.: The attachment of the synaptonemal complex to the nuclear envelope. Chromosoma (Berl.) 38, 405–417 (1972)
Hotta, Y., Shepard, J.: Biochemical aspects of colchicine action on meiotic cells. Molec. gen. Genet. 122, 243–260 (1973)
Leblond, C.P., Clermont, Y.: Definition of the stages of the cycle of the seminiferous epithelium in the rat. Ann. N.Y. Acad. Sci. 55, 548–573 (1952)
Martino, C. De, Capanna, E., Nicotra, M.R., Natali, P.G.: Immunochemical localization of contractile proteins in mammalian meiotic chromosomes. Cell Tissue Res. 213, 159–178 (1980)
Parvinen, L.M., Parvinen, M.: A “living cell method” for testing the early effects of antispermatogenic compounds: Model experiments with two alkylating agents thiotepa and nitrogen mustard. Int. J. Androl. Suppl. 2, 523–540 (1978)
Parvinen, L.M., Söderström, K.O., Parvinen, M.: Early effects of vinblastine and vincristine on the rat spermatogenesis: Analyses by a new transillumination-phase contrast microscopic method. Exp. Pathol. 15, 85–96 (1978)
Parvinen, M., Söderström, K.O.: Chromosome rotation and formation of synapsis. Nature 260, 534–535 (1976)
Parvinen, M., Vanha-Perttula, T.: Identification and enzyme quantitation of the stages of the seminiferous epithelial wave in the rat. Anat. Rec. 174, 435–450 (1972)
Rasmussen, S.W., Holm, P.B.: Mechanics of meiosis. Hereditas 93, 187–216 (1980)
Ritzén, E.M., Hansson, V., French, F.S.: The Sertoli cell. In: Comprehensive endocrinology. The testis. (H. Burger, D. de Kretser, eds.). pp. 171–194. New York: Raven Press 1981
Roosen-Runge, E.C.: Quantitative studies on spermatogenesis in the albino rat. II. The duration of spermatogenesis and some effects of colchicine. Am. J. Anat. 88, 163–176 (1951)
Söderström, K.O., Parvinen, M.: RNA synthesis in different stages of rat seminiferous epithelial cycle. Mol. Cell. Endocrinol. 5, 181–199 (1976)
Solari, A.J.: The spatial relationship of the X and Y chromosomes during meiotic prophase in mouse spermatocytes. Chromosoma (Berl.) 29, 217–236 (1970)
Wilson, L.: Properties of colchicine binding protein from chick embryo brain. Interactions with vinca alkaloids and podophyllotoxin. Biochemistry 9, 4999–5007 (1970)
Wilson, L., Friedkin, M.: The biochemical events of mitosis. I. Synthesis and properties of colchicine labeled with tritium in its acetyl moiety. Biochemistry 5, 2463–2468 (1966)
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Salonen, K., Paranko, J. & Parvinen, M. A colcemid-sensitive mechanism involved in regulation of chromosome movements during meiotic pairing. Chromosoma 85, 611–618 (1982). https://doi.org/10.1007/BF00330775
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DOI: https://doi.org/10.1007/BF00330775