Summary
The nucleotide sequence of a tobacco chloroplast gene coding for a protein homologous to Escherichia coli ribosomal protein S16 (rps16) has been determined. It is located 690 bp upstream from the gene for tRNALys (UUU) on the same DNA strand. This gene has an 860 bp intron. The intron boundary sequences are similar to those of the tobacco chloroplast split genes for tRNAGly (UCC), tRNALys (UUU), tRNAVal (UAC) and ribosomal proteins L2 and S12. We classified these introns as a new group, designated group III, which is different from groups I and II proposed by Michel and Dujon (1983). The conserved boundary sequences of the group III introns are GTGCGNY at the 5′ ends and ATCNRY(N)YYAY at the 3′ ends. The rps16 coding region contains 255 bp (85 codons) and its deduced amino acid sequence shows 34% homology (46% conservative replacements) with that of E. coli ribosomal protein S16. The primary transcript of rps16 is 1.3 kb long.
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Shinozaki, K., Deno, H., Sugita, M. et al. Intron in the gene for the ribosomal protein S16 of tobacco chloroplast and its conserved boundary sequences. Mol Gen Genet 202, 1–5 (1986). https://doi.org/10.1007/BF00330508
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DOI: https://doi.org/10.1007/BF00330508