Summary
Endothelial cells of the human umbilical vein were isolated by trypsin and cultured. Histological preparations of those umbilical cords were made to check the removal of the endothelium and not of other tissues.
The cells in culture grew in sheets which were identified with endothelial cells. Isolated cells rarely showed a spindle shape. Further investigation is needed to distinguish spindle-shaped cells from fibroblasts or other cellular elements.
Identification of endothelial cells in the previous reports and materials used are discussed.
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Read at the 12th Meeting of Japanese Tissue Culture Association, 25 November, 1961, Tokyo.
This research was conducted at The Tissue Culture Laboratory, Department of Anatomy, Tokyo University School of Medicine, under the direction of Prof. Junnosuke Nakai.
The author wishes to express his appreciation to Prof. Dr. Seiji Kimoto of the Surgical Department of Tokyo University, School of Medicine for his encouragement during the study.
Grateful acknowledgement is made to Prof. Dr. Junnosuke Nakai of the Department of Anatomy, Tokyo University, School of Medicine for his advice, supplying equipment in his tissue culture laboratory, and in the preparation of the manuscript.
The technical assistance of Miss Yoshiko Kawasaki is also gratefully acknowledged.
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Maruyama, Y. The human endothelial cell in tissue culture. Zeitschrift für Zellforschung 60, 69–79 (1963). https://doi.org/10.1007/BF00329383
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DOI: https://doi.org/10.1007/BF00329383