Abstract
Two thermostable enzymes produced by the thermophilic fungus Paecilomyces varioti, a chitinase and laminarinase, were used to isolate protoplasts of a thermophilic fungus, Malbranchea sulfurea. The frequency of protoplast regeneration observed (35%) was considerably higher than that obtained using commercial lytic enzymes.
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References
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Gautam, S.P., Gupta, A.K., Shrivastava, R. et al. Short communication: Protoplast formation from the thermophilic fungus Malbranchea sulfurea, using the thermostable chitinase and laminarinase of Paecilomyces varioti . World J Microbiol Biotechnol 12, 99–100 (1996). https://doi.org/10.1007/BF00327811
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DOI: https://doi.org/10.1007/BF00327811