Abstract
Five cDNA clones, harboring genetic messages preferentially expressed during the sexual differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was dependent on nitrogen starvation and their induction occurred at several stages of spore formation. Analysis of the cDNA prmary structures revealed a capacity for the coding of polypeptides of 19.2 kDa, 88.3 kDa, 60.1 kDa, 49.7 kDa, and 43.8 kDa, respectively. The translated amino-acid sequences of isp5 and isp6 were found to show significant similarities to those of amino-acid permeases and proteinase B of Saccharomyces cerevisiae, respectively. Disruption of isp6 arrested the cell cycle prior to conjugation and caused a drastic blocking effect on spore formation.
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Baker BS, Carpenter ATC, Esposito MS, Esposito RE, Sandler L (1976) The genetic control of meiosis. Annu Rev Genet 10:53–134
Benton WD Davis RW (1977) Screening λgt11 recombinant clones by hybridization to single plaques in situ. Science 196:180–182
Betz H, Weiser U (1976) Protein degradation and proteinases during yeast sporulation. Eur J Biochem 62:65–76
Clancy MJ, Buten-Magee B, Straight DJ, Kennedy AL, Partridge RM, Magee PT (1983) Isolation of genes exprssed preferentially during sporulation in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci USA 80:3000–3004
Cochran BH, Zullo AC, Verma IM, Stiles CD (1984) Expression of the c-fos gene and of a fos-related gene is stimulated by platelet-derived growth factor. Science 226:1080–1082
Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
Egel R (1971) Physiological aspects of conjugation in fission yeast. Planta 98:89–96
Egel R (1989) Mating-type genes, meiosis, and sporulation. In: Nasim A, Young P, Johnson BF (eds) Molecular biology of the fission yeast. Academic Press, Inc., New York, pp31–73
Egel R, Egel-Mitani M (1974) Premeiotic DNA synthesis in fission yeast. Exp Cell Res 88:127–134
Egel R, Nielsen O, Weilguny D (1990) Sexual differentiation in fission yeast. Trends Genet 6:369–373
Esposito RE, Klapholz S (1981) Meiosis and ascospore development. In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Sacharomyces: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 211–287
Fan J-B, Chikashige Y, Smith CL, Niwa O, Yanagida M, Cantor CR (1988) Construction of a NotI restriction map of the fission yeast Schizosaccharomyces pombe genome. Nucleic Acids Res 17:2801–2818
Grimm C, Kohli J, Murray J, Maundrell K (1988) Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker. Mol Gen Genet 215:81–86
Gutz H, Heslot H, Leupold U, Lopnieno N (1974) Schizosaccharomyces pombe In: King RD (ed) Handbook of genetics, vol 1. Plenum Press, New York, pp 395–446
Hoffmann W (1985) Molecular characterization of the CAN1 locus in Saccharomyces cerevisiae. J Biol Chem 260:11831–11837
Iino Y, Yamamoto M (1985) Negative control for the initiation of meiosis in Schizosaccharomyces pombe. Mol Gen Genet 198:416–421
Jany K-D, Lederer G, Mayer B (1986), Amino-acid sequence of proteinase K from the mold Tritirachium album Limber: Proteinase K — a subtilisin-related enzyme with disulfide bonds. FEBS Lett 199:139–144
Jauniaux J-C, Grenson M (1990) GAP1, the general amino-acid permease gene of Saccharomyces cerevisiae: nucleotide sequence, protein similarity with the other bakers yeast amino-acid permeases, and nitrogen catabolite repression. Eur J Biochem 190:39–44
Klar AJS, Halvorson HO (1975) Proteinase activities of Saccharomyces cerevisiae during sporulation. J Bacteriol 124:863–869
Malone RE (1990) Dual regulation of meiosis in yeast. Cell 61:375–378
Moehle CM, Tizard R, Lemmon SK Smart J, Jones EW (1987) Protease B of Saccharomyces cerevisiae isolation and regulation of the PRB1 structural gene. Mol Cell Biol 7:4390–4399
Moreno S, Klar A, Nurse P (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol 194:795–823
Nakanishi N, Yamamoto M (1984) Analysis of the structure and transcription of the aro3 cluster gene in Schizosacharomyces pombe. Mol Gen Genet 195:164–169
Percival-Smith A, Segall J (1984) Isolation of DNA sequences preferentially expressed during sporulation in Saccharomyces cerevisiae. Mol Cell Biol 4:142–150
Roberts MR, Robson F, Foster GD, Draper J, Scott RJ (1991) A Brassica napus mRNA expressed specifically in developing microspores. Plant Mol Biol 17:295–299
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Sherman F, Fink GR, Hicks JB (1986) Laboratory course manual for methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Shimoda C, Uehira M, Kishida M, Fujioka H, Iino Y, Watanabe Y, Yamamoto M (1987) Cloning and analysis of transcription of the mei2 gene responsible for initiation of meiosis in the fission yeast Schizosaccharomyces pombe. J Bacteriol 169:93–96
Smith CL, Cantor CR (1987) Purification specific fragmentation, and separation of large DNA molecules. Methods Enzymol 155:449–467
Smith E, DeLange R, Evans W, Landon M, Markland F (1968) Subtilisin Carlsberg. V. The complete sequence; comparison with subtilisin BPN′; evolutionary relationships. J Biol Chem 243:2184–2191
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
St. John TP, Davis RW (1979) Isolation of galactose inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridization. Cell 16:443–452
Tanaka J, Fink GR (1985) The histidine permease gene (HIP1) of Saccharomyces cerevisiae. Gene 38:205–214
Tatsumi H, Ogawa Y, Murakami S, Ishida Y, Murakami K, Masaki A, Kawabe H, Arimura H, Nakano E, Motai H (1989) A full-length cDNA clone for the alkaline protease from Aspergillus oryzae: structural analysis and expression in Saccharomyces cerevisiae. Mol Gen Genet 219:33–38
Watanabe Y, Iino Y, Furuhata K, Shimoda C, Yamamoto M (1988) The S. pombe mei2 gene encoding a crucial molecule for commitment to meiosis is under the regulation of cAMP. EMBO J 7:71–767
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Sato, S., Suzuki, H., Widyastuti, U. et al. Identification and characterization of genes induced during sexual differentiation in Schizosaccharomyces pombe . Curr Genet 26, 31–37 (1994). https://doi.org/10.1007/BF00326301
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DOI: https://doi.org/10.1007/BF00326301