Abstract
The mathematical technique of over-determined simultaneous equations has been applied to the analysis of chlorophyll extracts from plant materials. The choice of spectral range is important — it is shown that 50 data points in the range 500–700 nm give better results than a similar number in the range 400–700 nm, as they avoid interferences from carotenoids. Results agree with those calculated using Arnon's method (1949), but have a better precision of 2% rsd compared with 4%. The computing procedure also allows testing of the correctness of the results.
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Dedicated with best wishes to Professor Dr. K. Doerffel, on the occasion of his 70th birthday, remembering in particular how he helped to open cooperation with the west before reunification
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Marr, I.L., Suryana, N., Lukulay, P. et al. Determination of chlorophyll a and b by simultaneous multi-component spectrophotometry. Fresenius J Anal Chem 352, 456–460 (1995). https://doi.org/10.1007/BF00323366
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DOI: https://doi.org/10.1007/BF00323366