Summary
We have investigated the mechanism of action of the plant hormone ethylene by analyzing the expression of ethylene-inducible genes isolated from tomato (Lycopersicon esculentum). We have found that the expression of each cloned gene is regulated by ethylene in a unique manner. That is, for certain genes ethylene affects transcriptional processes, while for another gene it affects both transcriptional and post-transcriptional processes. Furthermore, induction of gene transcription by ethylene is organ specific for one gene, while for others it is not. In addition, we have measured gene expression as a function of ethylene concentration and have found that each gene displays a unique ethylene dose-response curve. Our results suggest that ethylene modulates gene expression by a variety of mechanisms.
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Abbreviations
- MG:
-
mature green
- [32P]nRNA:
-
in vitro 32P-labeled nuclear RNA transcripts
References
Abeles FB (1973) Ethylene in plant biology. Academic Press, New York, pp 1–302
Biale JB, Young RE (1981) Respiration and ripening in fruits-retrospect and prospect. In: Friend J, Rhodes MJC (eds) Recent advances in the biochemistry of fruit and vegetables. Academic Press, London, pp 1–39
Biggs MS, Harriman RW, Handa AK (1986) Changes in gene expression during tomato fruit ripening. Plant Physiol 81:395–403
Burg SP, Burg EA (1967) Molecular requirements for the biological activity of ethylene. Plant Physiol 42: 144–152
Cameron AC, Yang SF (1982) A simple method for the determination of resistance to gas diffusion in plant organs. Plant Physiol 70:21–23
Goldberg RB, Hoschek G, Tam SH, Ditta GS, Breidenbach RW (1981) Abundance, diversity, and regulation of mRNA sequence sets in soybean embryogenesis. Dev Biol 83:201–217
Grierson D (1985) Gene expression in ripening tomato fruit. CRC Crit Rev Plant Sci 3:113–132
Groudine M, Peretz M, Weintraub H (1981) Transcriptional regulation of hermoglobin switching in chicken embryos. Mol Cell Biol 1:281–288
Guilfoyle TJ (1986) Auxin-regulated gene expression in higher plants. CRC Crit Rev Plant Sci 4:247–276
Hagen G, Guilfoyle TJ (1985) Rapid induction of selective transcription by auxins. Mol Cell Biol 5:1197–1203
Hofer E, Darnell JE (1981) The primary transcription unit of the mouse Beta-major globin gene. Cell 23:585–593
Hofer E, Hofer-Warbinek R, Darnell JE (1982) Globin RNA transcription: a possible termination site and demonstration of transcriptional control correlated with altered chromatin structure. Cell 29:887–893
Katzenellenbogen BS, Gorski J (1972) Estrogen action in vitro. J Biol Chem 247:1299–1305
Kende H, Gardner G (1976) Hormone binding in plants. Annu Rev Plant Physiol 27:267–290
Key JL, Kroner P, Walker J, Hong LC, Ulrich TH, Ainley WM, Gantt JS, Nagao RT (1986) Auxin-regulated gene expression. Philos Trans R Soc Lond Series B. 314:427–440
Lieberman M (1979) Biosynthesis and action of ethylene. Annu Rev Plant Physiol 30:533–591
Lincoln JE, Cordes S, Read E, Fischer RL (1987) Regulation of gene expression by ethylene during Lycopersicon esculentum (tomato) fruit development. Proc Natl Acad Sci USA 84:2793–2797
Luthe DS, Quatrano RS (1980a) Transcription in isolated wheat nuclei I. Plant Physiol 65:305–308
Luthe DS, Quatrano RS (1980b) Transcription in isolated wheat nuclei II. Plant Physiol 65:309–313
Lyons JM, Pratt HK (1964) Effect of stage of maturity and ethylene treatment on respiration and ripening of tomato fruits. Proc Am Soc Hort Sci 84:491–500
Maunders MJ, Holdsworth MJ, Slater A, Knapp JE, Bird CR, Schuch W, Grierson D (1987) Ethylene stimulates the accumulation of ripening-related mRNAs in tomatoes. Plant Cell Environ 10:177–184
Nichols SE, Laties GG (1984) Ethylene-regulated gene transcription in carrot roots. Plant Mol Biol 3:393–401
Palmiter RD, Mulvihill ER, McKnight GS, Senear AW (1977) Regulation of gene expression in the chick oviduct by steroid hormones. Cold Spring Harbor Symp Quant Biol 42:639–649
Payvar F, DeFranco D, Firestone GL, Edgar B, Wrange O, Okret S, Gustafsson J, Yamamoto KR (1983) Sequence-specific binding of glucocorticoid receptor to MTV DNA at sites within and upstream of the transcribed region. Cell 35:381–392
Rhodes MJC (1980) The maturation and ripening of fruits. In: Thimann KV (ed) Senescence in plants. CRC Press, Boca Raton, pp 157–205
Scheidereit C, Beato M (1984) Contact between hormone receptor and DNA double helix within a glucocorticoid regulatory element of mouse mammary tumor virus. Proc Natl Acad Sci USA 81:3029–3033
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Su L-Y, McKeon T, Grierson D, Cantwell M, Yang SF (1984) Development of 1-aminocyclopropane-1-carboxylic acid synthase and polygalacturonase activities during maturation and ripening of tomato fruit. Hort Sci 19:576–578
Theologis A, Huynh TV, Davis RW (1985) Rapid induction of specific mRNAs by auxin in pea epicotyl tissue. J Mol Biol 183:53–68
Thomas PS (1983) Hybridization of denatured RNA transferred or dotted to nitrocellulose paper. Methods Enzymol 100:255–266
Tobin AJ (1979) Evaluating the contribution of posttranscriptional processing to differential gene expression. Dev Biol 68:47–58
von der Ahe D, Janich S, Scheidereit C, Renkawitz R, Schütz G, Beato M (1985) Glucocorticoid and progesterone receptors bind to the same sites in two hormonally regulated promoters. Nature 313:706–709
Walling L, Drews GN, Goldberg RB (1986) Transcriptional and posttranscriptional regulation of soybean seed protein mRNA levels. Proc Natl Acad Sci USA 83:2123–2127
Yamamoto KR, Stallcup MR, Ring J, Ringold GM (1977) Mammary tumor virus DNA: a glucocorticoid-responsive transposable element. Cold Spring Harbor Symp Quant Biol 42:625–638
Yang SF (1985) Biosynthesis and action ethylene. Hort Sci 20:41–45
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Communicated by R.B. Goldberg
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Lincoln, J.E., Fischer, R.L. Diverse mechanisms for the regulation of ethylene-inducible gene expression. Molec. Gen. Genet. 212, 71–75 (1988). https://doi.org/10.1007/BF00322446
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DOI: https://doi.org/10.1007/BF00322446