Summary
The transformation of spindle-like spermatids into tubular forms in Notodromas monacha (Ostracoda) has been studied by means of phase contrast and electron microscopy. At each of two opposite sides of the nucleus of spindle-like spermatids, two extensions of the nuclear membrane arise. These extensions form two “Flügelstrukturen“ (wing-like structures) obviously by participation of vesicles of the endoplasmic reticulum. Each “Flügelstruktur“ consists of two lamellae, each bearing numerous tubules. The tube-like spermatids consist of a large body and a small thread. The nucleus, the “Nebenkern“-derivatives and the “Flügelstrukturen“ extend through the whole body. Within the thread only the nucleus and the outermost parts of the “Flügelstrukturen“ are found. A columnar body, supposed to be chromatin, is located within that part of the nucleus which is situated in the free end of the body. A dense axial rod extends between the columnar body and the free end of the thread. The dorso-lateral sphere of the nucleus contains electron dense particles of unknown function. As soon as the construction of the “Flügelstrukturen“ is finished, the endoplasmic reticulum and the microtubules are reduced. Simultaneously the double-membrane surrounding the axial rod disappears. The “Flügelstrukturen“ are interpreted as organelles responsible for the motility of the sperm.
Zusammenfassung
Bei Notodromas monacha (Ostracoda) wird die Entwicklung der Spermatiden von der Spindelbis zur Schlauchform mit Phasenkontrast und elektronenmikroskopisch untersucht. Am Kern der spindelförmigen Spermatiden sind an zwei gegenüberliegenden Seiten jeweils zwei Ausstülpungen der Membran zu beobachten. Aus ihnen werden offenbar unter Beteiligung von Vesikeln des endoplasmatischen Reticulum zwei Flügelstrukturen aufgebaut. Jede Flügelstruktur besteht aus zwei Lamellen, die mit tubulären Elementen besetzt sind. An den schlauchförmigen Spermatiden läßt sich ein dicker Körper und ein dünner Faden unterscheiden. Kern, Nebenkern-Derivate und Flügelstrukturen durchziehen den Körper der Länge nach. Der Faden enthält nur noch den Kern und die Ausläufer der Flügelstrukturen. Im Bereich des freien Körperendes liegt im Kern ein als Chromatin gedeuteter Kolben. Diesem folgt in Richtung zum Fadenende ein dichter Achsenstab, der von einer Doppelmembran umhüllt ist. Im dorso-lateralen Kernraum finden sich elektronendichte Brocken unbekannter Bedeutung. Nach Beendigung des Aufbaues der Flügelstrukturen wird das endoplasmatische Reticulum samt Mikrotubuli weitgehend abgebaut. Gleichzeitig verschwindet die den Achsenstab umhüllende Doppelmembran. Die Flügelstrukturen werden als das für die Motilität der Spermien verantwortliche Organell gedeutet.
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Zissler, D. Die Spermiohistogenese des Süßwasser-Ostracoden Notodromas monacha O. F. Müller. Z. Zellforsch. 96, 106–133 (1969). https://doi.org/10.1007/BF00321481
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DOI: https://doi.org/10.1007/BF00321481