Summary
Forty-eight patients with acute leukaemia in relapse (n=14), acute leukaemia in complete remission (n=19), chronic myeloid leukaemia (n=8) or severe aplastic anaemia (n=7) received a marrow transplant. The first 26 patients were nursed in laminar-air-flow plastic isolators while the next 22 patients were treated in barrier nursing rooms. Gnotobiotic parameters and morbidity in the 2 groups are compared. Good decontamination of the gastro-intestinal tract was obtained using either of the 2 isolation techniques. The incidence of bacterial and mycotic infections, as well as the supportive care required by the patients was almost equal in both groups. Our results also suggest that the incidence of graft versus host disease may decrease with efficient decontamination of the patients.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Baird-Parker AC (1972) Classification and identification of staphylococci and their resistance to physical agents. In: Cohen JE (ed) The staphylococci. Wiley, New York, p 1
Bekkum DW van, Vries MJ de, Waaij D van der (1967) Lesions characteristic of secondary disease in germfree heterologous radiation chimeras. J Natl Cancer Inst 38: 223–231
Bekkum DW van, Knaan S (1977) Role of bacterial microflora in development of intestinal lesions from graft-versus-host reaction. J Natl Cancer Inst 58: 787–790
Bekkum DW van, Roodenburg J, Heidt PJ, Waaij D van der (1974) Mitigation of secondary disease of allogeneic mouse radiation chimeras by modification of the intestinal microflora. J Natl Cancer Inst 52: 401–404
Buckner CD, Clift RA, Sanders JE, Meyers JD, Counts GW, Farewell VT, Thomas ED (1978) Protective environment for marrow transplant recipients. A prospective study. Ann Intern Med 89: 893–901
Buckner CD, Clift RA, Sanders JE, Thomas ED (1978) The role of a protective environment and prophylactic granulocyte transfusions in marrow transplantation. Transplant Proc 10: 255–257
Chüden H, Haralambie E, Linzenmeier G (1970) Der Einfluß von Amphotericin B auf den Candida-Befall der Mundhöhle und des Darmes bei Tetracyclin-Therapie. Mykosen 13: 13–17
Dankert J, Gaus W, Gaya H, Krieger D, Linzenmeier G, Waaij D van der (1978) Protective isolation and antimicrobial decontamination in patients with high susceptibility to infection. A prospective cooperative study of gnotobiotic care in acute leukaemia patients. III. The quality of isolation and decontamination. Infection 6: 175–191
Dietrich M, Gaus W, Vossen J, Waaij D van der, Wendt F (1977) Protective isolation and antimicrobial decontamination in patients with high susceptibility to infection. A prospective cooperative study of gnotobiotic care in acute leukaemia patients. I. Clinical results. Infection 5: 107–114
Essers L, Haralambie E (1977) Erfahrungen mit dem API 20A-Testsystem bei der Identifizierung von Anaerobiern aus der täglichen Routine. Zentralbl Bakteriol (Orig A) 238: 394–401
Haralambie E, Essers L (1977) Reicht das API 20A-Testsystem zur Differenzierung der in der Routine anfallenden anaeroben Spezies aus? Ärztl. Lab. 23: 317
Haralambie E, Linzenmeier G, Nowrousian MR, Schaefer UW, Schmidt, CG (1980) Gnotobiotische Untersuchungen — ein mikrobiologischer Beitrag zur Dekontamination von knochenmarktransplantierten Leukämiepatienten. Immun Infekt 8: 89–95
Heit H, Wilson R, Fliedner TM, Kohne E (1973) Mortality of secondary disease in antibiotic-treated mouse radiation chimeras. In: Heneghan JB (ed) Germfree research: biological effects of gnotobiotic environments. Academic Press, New York, pp 477–485
Jones JM, Wilson R, Bealmear PM (1971) Mortality and gross pathology of secondary disease in germfree mouse radiation chimeras. Radiat Res 45: 577–588
Kurrle E, Bhaduri S, Heimpel H, Hoelzer D, Krieger D, Vanek E, Kubanek B (1980) The efficiency of strict reverse isolation and antimicrobial decontamination in remission induction therapy of acute leukemia. Blut 40: 187–195
Linzenmeier G, Haralambie E, Dermouni H (1979) Kurzfristige orale Chemoprophylaxe vor Operationen am Darm. Aussagemöglichkeiten bei der quantitativen Untersuchung der Stuhlflora auf Bakterien und Pilze. Zentralbl Bakteriol (Orig A) 243: 326–335
Nowrousian MR, Schaefer UW, Schmidt CG, Haralambie E, Linzenmeier G, Hantschke D, Öhl S, Beyer JH (1979) Gnotobiotic results in bone marrow transplantation. In: Fliedner TM, Heit H, Niethammer D, Pflieger H (eds) Clinical and experimental gnotobiotics. Zentralbl Bakteriol [Suppl 7] G. Fischer, Stuttgart, New York, pp 275–279
Schaefer UW, Schüning F (1983) Knochenmarktransplantation am Westdeutschen Tumorzentrum Essen. In: Schmidt CG (ed): Aktuelle Probleme der Hämatologie und internistischen Onkologie. Präklinische und klinische Untersuchungen. Springer Berlin Heidelberg New York, pp 113–124
Schneider H, Haralambie E, Towfigh H, Eigler FW, Linzenmeier G (1976) Über die Wirksamkeit der Antibiotikavorbereitung in der Colonchirurgie. Chirurg 47: 33–38
Storb R, Prentice RL, Buckner CD, Clift RA, Appelbaum F, Deeg HJ, Doney K, Hansen JA, Mason M, Sanders JE, Singer J, Sullivan KE, Witherspoon RP, Thomas ED (1983) Graft-versus-host-disease and survival in patients with aplastic anemia treated by marrow grafts from HLA-identical siblings. Beneficial effect of a protective environment. N Engl J Med 308: 302–307
Storb R, Thomas ED (1983) Allogeneic bone marrow transplantation Immunological Rev 71: 77–102
Yates JW, Holland JF (1973) A controlled study of isolation and endogenous microbial suppression in acute myelocytic leukemia patients. Cancer 32: 1490–1498
Author information
Authors and Affiliations
Additional information
Supported by Deutsche Forschungsgemeinschaft SFB 102
Rights and permissions
About this article
Cite this article
Mahmoud, H.K., Schaefer, U.W., Schüning, F. et al. Laminar air flow versus barrier nursing in marrow transplant recipients. Blut 49, 375–381 (1984). https://doi.org/10.1007/BF00319885
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00319885