Summary
Red cells (RBC) possessing the low-frequency MNSs antigen Dantu from 36 Black individuals (21 propositi) were found to exhibit the NE variety of this phenotype, as judged from the electrophoretic glycophorin (GP) pattern, described in an accompanying article, and/or from the polybrene test which detects the decreased NeuAc level of these RBC. All known DantuNE RBC (53) exhibit the phenotype M+N+. This finding as well as family studies and immunochemical investigations demonstrate that the Dantu NEallele encodes a blood group M-specific GP A. Thus, the strongly decreased GP A level of RBC from Dantu NEheterozygotes represents the product of the Dantu allele and its normal counterpart. It is suggested that the formation of a complex with the anion channel protein (band 3) represents the prerequisite for optimum incorporation of GP A into normal RBC membranes. The hybrid GP in DantuNE RBC, produced in large quantity, might suppress the incorporation of GP A in a cis and trans manner via the formation of a complex with band 3. The hybrid GP in DantuNE RBC lacks U activity, but expresses N activity and a qualitatively altered s antigen, thus proving its GP B-GP A hybrid nature in conjunction with data described in the accompanying article. Screening of ficin-treated RBC with Vicia lectin revealed that the Dantu phenotype exhibits a frequency of about 0.005 in American Blacks and less than 0.001 in Germans.
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Blanchard D, Asseraf A, Prigent MJ, Cartron JP (1983) Miltenberger Class I and II erythrocytes carry a variant of glycophorin A. Biochem J 213: 399–404
Blanchard D, Asseraf A, Prigent MJ, Moulds JJ, Chandanayingyong, Cartron JP (1984) Interaction of Vicia graminea anti-N lectin with cell surface glycoproteins from erythrocytes with rare blood group antigens. Hoppe-Seyler's Z Physiol Chem 365: 469–478
Chang JY, Creaser EH, Hughes GJ (1977) Separation of 4-N,N-dimethylaminoazobenzene-4′-thiohydantoins of amino acids by thin-layer chromatography on silica gel. J Chromatogr 140: 125–128
Chang JY, Brauer D, Wittmann-Liebold B (1978) Micro-sequence analysis of peptides and proteins using 4-NN-dimethylaminoazobenzene-4′-isothiocyanate/phenylisothiocyanate double coupling method. FEBS Lett 93: 205–214
Contreras M, Green C, Humphreys J, Tippett P, Daniels G, Teesdale P, Armitage S, Lubenko A (1984) Serology and genetics of an MNSs-associated antigen Dantu. Vox Sang 46: 377–386
Dahr W (1981) Serology, genetics and chemistry of the MNSs blood group system. Rev Franc Transf Immunohematol 24: 85–95
Dahr W (1986) Immunochemistry of sialoglycoproteins in human red blood cell membranes. In: Vengelen-Tyler V, Judd WJ (eds) Recent advances in blood group biochemistry. American Association of Blood Banks, Arlington, VA pp 23–65
Dahr W, Krüger J (1983) Solubilization of various blood group antigens by Triton X-100. In: Proceedings of the 10th International Congress of the Society for Forensic Haemogenetics, Munich. Schmitt & Meyer, Würzburg, pp 141–146
Dahr W, Uhlenbruck G, Leikola J, Wagstaff W, Landfried K (1976) Studies on the membrane glycoprotein defect of En (a-) erythrocytes. I. Biochemical aspects. J Immunogenet 3: 329–346
Dahr W, Beyreuther K, Steinbach H, Gielen W, Krüger J (1980) Structure of the Ss blood group antigens. II. A methionine/threonine polymorphism within the N-terminal sequence of the Ss glycoprotein. Hoppe-Seyler's Z Physiol Chem 361: 895–906
Dahr W, Weber W, Kordowicz M (1981) The allele Su in Caucasians — medico-legal, immunochemical and genetic aspects. In: Hummel K, Gerchow J (eds) Biomathematical evidence of paternity. Springer, Berlin, Heidelberg, New York, pp 131–138
Dahr W, Newman RA, Contreras M, Kordowicz M, Teesdale P, Beyreuther K, Krüger J (1984) Structures of Miltenberger class I and II specific major human erythrocyte membrane sialoglycoproteins. Eur J Biochem 138: 259–265
Dahr W, Moulds J, Baumeister G, Moulds M, Kiedrowski S, Hummel M (1985) Altered membrane sialoglycoproteins in human erythrocytes lacking the Gerbich blood group antigens. Biol Chem Hoppe-Seyler 366: 201–211
Dahr W, Moulds J, Unger P, Blanchard D, Cartron JP (1985) Serological and biochemical investigations on the N.E. variety of the Dantu red cell phenotype. In: Brinkmann B, Henningsen K (eds) Advances in forensic haemogenetics 1. 11th Congress of the Society for Forensic Haemogenetics, Copenhagen. Springer, Berlin, Heidelberg, New York, pp 1–7
Dahr W, Kordowicz M, Moulds J, Gielen W, Lebeck L, Krüger J (1986) Characterization of the Ss sialoglycoprotein and its antigens in Rhnull erythrocytes. Blut 54: 13–24
Dahr W, Wilkinson S, Issitt PD, Beyreuther K, Hummel M, Morel P (1986) High frequency antigens of human erythrocyte membrane sialoglycoproteins. III. Studies on the EnaFR, Wrb and Wra antigens. Biol Chem Hoppe-Seyler 367: 1033–1045
Dahr W, Moulds J, Unger P, Kordowicz M (1987) The Dantu erythrocyte phenotype of the NE variety. I. Dodecylsulfate polyacrylamide gel electrophoretic studies. Blut 55: 19–31
Duk M, Lisowka E, Kordowicz M, Wasniowska K (1982) Studies on the specificity of the binding site of Vicia graminea anti-N lectin. Eur J Biochem 123: 105–112
Fairbanks G, Steck TL, Wallach DFH (1971) Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10: 2606–2617
Furthmayr H, Tomita M, Marchesi VT (1975) Fractionation of the major sialoglycopeptides of the human red membrane. Biochem Biophys Res Commun 65: 113–121
Glöckner WM, Newman RA, Dahr W, Uhlenbruck G (1976) Alkali-labile oligosaccharides from glycoproteins of different erythrocyte and milk fat globule membranes. Biochim Biophys Acta 443: 402–413
Habibi B, Fouillade MT, Duedari N, Issitt PD, Tippett P, Salmon C (1978) The antigen Duclos. Vox Sang 34: 302–309
Issitt PD (1985) Applied blood group serology. 3rd ed. Montgomery Scientific Publications, Miami, FL
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Laird-Fryer B, Moulds JJ, Wagoner LK (1984) Hemolytic disease of the newborn due to anti-Dantu. In: Cooper ES (ed) Journal of the South Central Association of Blood Banks, Austin, TX, 25: 16 (abstract)
Lisowska E (1987) Antigenic properties of human erythrocyte glycophorins. In: Wu AM (ed) Molecular immunology of complex carbohydrates. Plenum Press, New York NY (in the press)
Marsh WL (1972) Scoring of hemagglutination reactions. Transfusion 12: 352–354
Mawby WJ, Anstee DJ, Tanner MJA (1981) Immunochemical evidence for hybrid sialoglycoproteins of human erythrocytes. Nature 291: 161–162
Merry AH, Hodson C, Thomson E, Mallinson G, Anstee DJ (1986) The use of monoclonal antibodies to quantify the levels of sialoglycoproteins α and δ and variant sialoglycoproteins in human erythrocyte membranes. Biochem J 233: 93–98
Moores P, Dinnematin H, Smart E (1984) Dantu and MNSs blood groups in a large natal family. In: 18th Congress of the International Society of Blood Transfusion, Munich. S. Karger, Basel, p 166 (abstract P 8–14)
Moulds J, Dahr W (1987) MNSs and Gerbich blood group systems. In: Litwin SD, Scott D, Flaherty L, Reisfeld RA, Marcus DM (eds) Human immunogenetics: An advanced text. Marcel Dekker Inc., New York NY (in the press)
Moulds JJ, Case J, Moulds M, Northam C (1986) Heterogeneity of anti-s disclosed by red blood cells from Dantu/S uindividuals. In: Abstract volume of the 19th Congress of the International Society of Blood Transfusion, p 653 (abstract P-F-311-9)
Nigg EA, Bron C, Girardet M, Cherry RJ (1980) Band 3-glycophorin A association in erythrocyte membranes demonstrated by combining protein diffusion measurements with antibody-induced cross-linking. Biochemistry 19: 1887–1893
Pilkington P, Dahr W, Lacey P, Moulds JJ, Wheeler N (1985) A Dantu-positive Caucasian demonstrating a variant hybrid sialoglycoprotein. Transfusion 25: 464 (abstract)
Rubin H (1963) Antibody elution from red cells. J clin Pathol 16: 70–72
Salmon C, Cartron JP, Rouger P (1984) The human blood groups. Masson Publishing USA Inc., New York NY
Sondag D, Alloisio N, Blanchard D, Ducluzeau MT, Colonna P, Bachir D, Bloy C, Cartron JP, Delauney J (1987) Gerbich reactivity in 4.1 (-) hereditary elliptocytosis and protein 4.1 level in blood group Gerbich deficiency. Br J Haematol 65: 43–50
Tanner MJA, Anstee DJ, Mawby WJ (1980) A new human erthrocyte variant (Ph) containing an abnormal membrane sialoglycoprotein. Biochem J 187: 493–500
Tomita M, Furthmayr H, Marchesi VT (1978) Primary structure of human erythrocyte glycophorin A. Isolation and characterization of peptides and complete amino acid sequence. Biochemistry 17: 4756–4770
Unger P, Orlina A, Dahr W, Kordowicz M, Moulds J, Newman RA (1981) Two new gene complexes in the MNSs blood group system. In: Engelfriet CP (ed) Proceedings of the 9th International Congress of the Society for Forensic Haemogenetics. Schmitt & Meyer, Würzburg, pp 189–193
Vengelen-Tyler V, Mogck N (1986) A new test useful in identifying red cells with a (α-δ) hybrid sialoglycoprotein. Transfusion 26: 231–233
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Preliminary results of this work were presented at the 9th and the 11th International Congress of the Society for Forensic Haemogenetics [14, 40], the 26th Annual Meeting of the South Central Association of Blood Banks [25] and the 19th Congress of the International Society of Blood Transfusion [32]
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Unger, P., Procter, J.L., Moulds, J.J. et al. The dantu erythrocyte phenotype of the NE variety. Blut 55, 33–43 (1987). https://doi.org/10.1007/BF00319639
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DOI: https://doi.org/10.1007/BF00319639