Summary
The Fo-ATPase subunit 6 gene (atp6) of rapeseed mitochondria has been isolated from both pol malesterile and normal (fertile) cytoplasms in order to determine whether the rearrangements around the atp6 locus in pol male-sterile cytoplasm play a role in cytoplasmic male-sterility (cms). The pol cms and normal atp6 genes are identical and encode a 261-amino acid polypeptide. As a result of extensive rearrangement, a novel reading frame (pol-urf) was generated upstream of the atp6 gene only in pol cms mitochondria, which encoded 105 amino acids and might be co-transcribed with atp6. A 5′-portion of pol-urf shows sequence homology to the Oenothera ORFB gene associated with coxIII. A 5′-flanking region of the pol-urf also shows homology to that of ORF105 in Ogura cms radish mitochondria. These DNA rearrangements which give rise to pol-urf in the vicinity of the atp6 locus may be responsible for cms in rapeseed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bailey-Serres J, Hanson DK, Fox TD, Leaver CJ (1986) Cell 47:567–576
Bland MM, Levings CS III, Matzinger DF (1987) Curr Genet 12:475–481
Dewey RE, Levings CS III, Timothy DH (1985) Plant Physiol 79:914–919
Dewey RE, Levings CS III, Timothy DH (1986) Cell 44:439–449
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Fu TD (1981) Eucarp Crucif Newslett 6:6–7
Handa H, Ohkawa Y, Nakajima K (1990) Jpn J Genet 65:17–24
Hanson MR, Conde MF (1985) Int Rev Cytol 94:213–267
Hiesel R, Schobel W, Schuster W, Brennicke A (1987) EMBO J 6:29–34
Ikeda TM, Ishii K, Terachi T, Tsunewaki K (1990) Jpn J Genet 65:435
Kadowaki K, Suzuki T, Kazama S, Oh-fuchi T, Sakamoto W (1989) Nucleic Acids Res 17:7519
Kadowaki K, Suzuki T, Kazama S (1990) Jol Gen Genet 224:10–16
Kazama S, Suzuki T, Kadowaki K, Akihama T (1990) Nucleic Acids Res 18:5879
Lonsdale DM (1987) Plant Physiol Biochem 25:265–271
Lansdale DM, Hodge TP, Fauron CMR (1984) Nucleic Acids Res 12:9249–9261
Makaroff CA, Palmer JD (1988) Mol Cell Biol8:1474–1480
Makaroff CA, Apel IJ, Palmer JD (1989) J Biol Chem 264:11706–11713
Makaroff CA, Apel IJ, Palmer JD (1990) Plant Mol Biol 15:735–746
Morikami A, Nakamura K (1987) J Biochem 101:967–976
Palmer JD (1988) Genetics 118:341–351
Palmer JD, Herbon A (1988) J Mol Evol 28:87–97
Palmer JD, Shields CR (1984) Nature 307:437–440
Quagliariello C, Saiardi A, Gallerani R (1990) Curr Genet 18:355–363
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Schuster W, Brennicke A (1987) Nucleic Acids Res 15:9092
Shiga T, Baba S (1971) Jpn J Breed 21 (suppl 2):16–17 (in Japanese)
Shiga T, Baba S (1973) Jpn J Breed 23:187–197 (in Japanese with English summary)
Southern E (1980) Methods Enzymol 68:152–176
Stern DB, Newton KJ (1986) Methods Enzymol 118:488–496
Thompson KF (1972) Heredity 29:253–257
Witt U, Hansen M, Albaum M, Abel WO (1991) Curr Genet 19:323–327
Young EG, Hanson MR (1987) Cell 50:41–49
Young EG, Hanson MR, Dierks PM (1986) Nucleic Acids Res 14:7995–8006
Author information
Authors and Affiliations
Additional information
Communicated by C. W. Birky, Jr.
Rights and permissions
About this article
Cite this article
Handa, H., Nakajima, K. Different organization and altered transcription of the mitochondrial atp6 gene in the male-sterile cytoplasm of rapeseed (Brassica napus L.). Curr Genet 21, 153–159 (1992). https://doi.org/10.1007/BF00318475
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00318475