Abstract
The interaction of aluminium ion species with soluble protein kinase C, Ca2+/phospholipid-dependent protein kinase, from mouse brain has been examined in vitro. The activity of protein kinase C was increased by addition of Ca2+ displaying an EC50 value of 10.3±1.1 × 10−6 M. The A1 species inhibited the activity with an IC50 values of 8.6±0.5 × 10−5 M and 2.7±0.3 × 10−5 M in the presence of 0.5 mM Ca2+ and absence of Ca2+, respectively. Concerning the EC50 for Ca2+ activation, this was increased by the A1 species in a dose-dependent manner. Moreover, the inhibition was of a non-competitive type with respect to H1 histone and of a mixed type with respect to ATP. It is likely that the inhibition was caused by 1) the blocking of Mg2+ binding to ATP, 2) the blocking of Ca2+ binding to protein kinase C. Our results suggested that protein kinase C was involved in neurotoxicity of A1.
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Katsuyama, H., Saijoh, K., Inoue, Y. et al. The interaction of aluminium with soluble protein kinase C from mouse brain. Arch Toxicol 63, 474–478 (1989). https://doi.org/10.1007/BF00316451
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DOI: https://doi.org/10.1007/BF00316451