Summary
Cholesterol and calcium phosphate, the latter in the form of hydroxyapatite, accumulate in atherosclerotic lesions. In this report, we demonstrate that these organic and inorganic constitutents of lesions can accumulate together, closely associated in crystal agglomerates. Using the fluorescent cholesterol probe, filipin, we identified unesterified cholesterol that was associated with calcium granules in tissue sections of lesions. We also have shown that small crystallites of cholesterol can associate with preformed hydroxyapatite crystals in vitro. Scanning electron microscopy couple with energy-dispersive X-ray analysis demonstrated the physical association of many small crystallites of cholesterol with larger crystals of hydroxyapatite. These small crystallites of cholesterol associated with hydroxyapatite stained with filipin. This contrasted with the lack of filipin staining of unassociated larger cholesterol crystals or hydroxyapatite alone. How cholesterol and calcium come to be closely associated in crystal agglomerates within atherosclerotic lesions remains to be determined.
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References
Bornig H, Geyer G (1974) Staining of cholesterol with the fluorescent antibiotic filipin. Acta Histochem 50:110–115
Kruth HS (1984) Localization of unesterified cholesterol in human atherosclerotic lesions. Am J Pathol 114:201–208
Tanimura A, McGregor DH, Anderson HC (1986) Calcification in atherosclerosis I. Human studies. J Exp Pathol 2:261–273
Blumenthal HT, Lansing AI, Wheeler PA (1944) Calcification of the media of the human aorta and its relation to intimal arterio-sclerosis, ageing and disease. Am J Pathol 20:665–687
Moon JY (1972) Factors affecting arterial calcification associated with atherosclerosis. Atherosclerosis 16:119–126
Tomazic BB, Brown WE, Queral LA, Sadovnik M (1988) Physicochemical characterization of cardiovascular calcified deposits. Atherosclerosis 69:5–19
Berberian PA, Fowler S (1979) The subcellular biochemistry of human arterial lesions: I. Biochemical constituents and marker enzymes in diseased and unaffected portions of human aortic specimens. Exp Mol Pathol 30:27–40
Hirsch D, Azoury R, Sarig S (1990) Co-crystallization of cholesterol and calcium phosphate as related to atherosclerosis. J Crystal Growth 104:759–765
Lerner E, Azoury R, Sarig S (1989) Rapid precipitation of apatite from ethanol-water solutions. J Crystal Growth 97:725–730
DeKruijff B, Demel RA III (1974) Molecular structure of the polyene antibiotic-cholesterol complexes. Biochim Biophys Acta 339:57–70
Craven BM (1976) Crystal structure of cholesterol monohydrate. Nature 260:727–729
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Hirsch, D., Azoury, R., Sarig, S. et al. Colocalization of cholesterol and hydroxyapatite in human atherosclerotic lesions. Calcif Tissue Int 52, 94–98 (1993). https://doi.org/10.1007/BF00308315
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DOI: https://doi.org/10.1007/BF00308315