Summary
A comparative study on the fine structure, phagocytic properties, and peroxidatic activity of guinea pig resident peritoneal macrophages and the monocytes and macrophages present in experimentally induced sterile peritoneal exudates is reported. A number of fine-structural characteristics of the resident macrophages are described, including a peculiar configuration of the cell surface termed “loops”. The structure of these loops suggests the existence of a cell coat on the cytoplasmic side of the plasma membrane.
The following observations were also made. Exudate monocytes differ from resident peritoneal macrophages mainly by the limitation of peroxidatic activity to cytoplasmic granules. The resident peritoneal macrophages have peroxidatic activity in the endoplasmic reticulum, the nuclear envelope, the annulate lamellae, and some of the cisternae of the Golgi apparatus. Macrophages derived from exudate monocytes do not show the same distribution of peroxidatic activity as the resident peritoneal macrophages. Both types of macrophages are highly active in the uptake of exogenous material. The functional significance of these observations is discussed and the conclusion is drawn that peritoneal exudates contain two different types of macrophages: resident macrophages and exudate macrophages.
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With the assistance of E.J. van Lohuizen.
Acknowledgements. The authors are greatly indebted to Mrs. E.G.J. Meeuwissen-van der Vlugt, Mr. L. Verschragen, and Mr. J. Beentjes for their skillful technical assistance and to Drs. E. Wisse and J. J. Emeis for their discussion of the manuscript.
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Daems, W., Brederoo, P. Electron microscopical studies on the structure, phagocytic properties, and peroxidatic activity of resident and exudate peritoneal macrophages in the guinea pig. Z.Zellforsch 144, 247–297 (1973). https://doi.org/10.1007/BF00307305
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DOI: https://doi.org/10.1007/BF00307305