Summary
Clone banks of PvuII, BamHI and XhoI fragments were generated of the Solanum tuberosum cv Katahdin plastome. These clone banks, in conjunction with molecular hybridization to tobacco ctDNA probes, were used to construct a physical map of potato ctDNA. The potato plastome was found to be a circular molecule of 155–156 Kbp containing two inverted repeat regions of 23–27 Kbp. The arrangement of restriction sites is very similar to that of other Solanaceae plastomes. Heterologous hybridization to known ctDNA encoded gene probes from tobacco allowed us to establish a genetic map of the potato chloroplast genome. The arrangement of these genes on the potato plastome resembles that on most higher plant ctDNAs.
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Anonymous (1984) Potatoes for the developing world. International Potato Center (CIP), Lima, Peru, p 148
Bovenberg WA, Howe CJ, Cool AJ, Nijkamp HJJ (1984) Physical mapping of genes for chloroplast DNA encoded subunit polypeptides of the ATPsynthase complex from Petunia hybrida. Curr Genet 8:283–290
Buckner B, Hyde BB (1985) Chloroplast DNA variation between the common cultivated potato (Solanum tuberosum ssp. tuberosum) and several South American relatives. Theor Appl Genet 71:527–531
Cannon GC, Heinhorst S, Weissbach A (1985) Quantitative molecular hybridization on nylon membranes. Anal Biochem 149:229–237
Cohen BN, Coleman TA, Schmitt JJ, Weissbach H (1984) In vitro expression and characterization of the translation start site of the psbA gene product (Q B protein) from higher plants. Nucleic Acids Res 12:6221–6230
Cordingly JS, Taylor DW, Dunne DW, Butterworth AE (1983) Clone banks of cDNA from the parasite Schistosoma mansoni: isolation of clones containing a potentially immunodiagnostic antigen gene. Gene 26:25–39
Dagbert M, Ehrlich SD (1979) Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28
Fluhr R, Edelman M (1981 a) Conservation of sequence arrangement among the Solanaceae and between Nicotiana and Spinacia. Nucleic Acids Res 9:6841–6853
Fluhr R, Edelman M (1981 b) Physical mapping of Nicotiana tabacum chloroplast DNA. Mol Gen Genet 181:484–490
Fluhr R, Fromm H, Edelman M (1983) Clone bank of Nicotiana tabacum chloroplast DNA: mapping of the alpha, beta and epsilon subunits of the ATPase coupling factor, the large subunit of ribulosebisphosphate carboxylase, and the 32-KDal membrane protein. Gene 25:271–280
Fromm H, Edelman M, Koller B, Goloubinoff P, Galun E (1986) The enigma of the gene coding for ribosomal protein S12 in the chloroplasts of Nicotiana. Nuclei Acids Res 14:883–898
Galun E, Aviv D (1986) Organelle transfer. Methods Enzymol 118:595–611
Green RM, Vardi A, Galun E (1986) The plastome of Citrus. Physical map, variation among Citrus cultivars and species and comparison with related genera. Theor Appl Genet 72:170–177
Hawkes JG (1978) Biosystematics of the potato. In: Harris PM (ed) The potato crop. Chapman and Hall, London, pp 15–69
Hetherington SE, Smillie RM, Malagamba P, Huaman Z (1983) Heat tolerance and cold tolerance of cultivated potatoes measured by chlorophyll-fluorescence method. Planta 159:119–124
Hildebrand M, Jurgenson JE, Ramage RT, Bourgue DP (1985) Derivation of a physical map of chloroplast DNA from Nicotiana tabacum by two-dimensional gel and computer-aided restriction analysis. Plasmid 14:64–79
Hosaka K (1986) Who is the mother of the potato? — restriction endonuclease analysis of chloroplast DNA of cultivated potato. Theor Appl Genet 72:606–618
Hosaka K, Ogihara Y, Matsubayashi M, Tsunewaki K (1984) Phylogenetic relationships between the tuberous Solanum species as revealed by restriction endonuclease analysis of chloroplast DNA. Jpn J Genet 59:349–369
Kahn M, Kolter R, Thomas C, Figurski D, Meyer R, Remaut E, Helinski DR (1979) Plasmid cloning vehicles derived from plasmids ColEI, F, R6K. In: Wu R (ed) Methods in enzymology, vol 68. pp 268–280
Koller B, Fromm H, Galun E, Edelman M (1987) Evidence for in vivo trans-splicing of pre-mRNAs in tobacco chloroplasts. Cell 48:111–119
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory
Norrander J, Kempe T, Messing J (1983) Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26:101–106
Palmer JD (1985) Comparative organization of chloroplast genomes. Annu Rev Genet 19:325–354
Palmer JD (1986) Isolation and structural analysis of chloroplast DNA. In: Weissbach A, Weissbach H (eds) Methods in enzymology, vol 118. pp 167–185
Palmer JD, Osorio B, Aldrich J, Thompson WF (1987) Chloroplast DNA evolution among legumes: loss of a large inverted repeat occurred prior to other sequence rearrangements. Curr Genet 11:275–286
Palmer JD, Zamir D (1982) Chloroplast DNA evolution and phylogenetic relationship in Lycopersicon. Proc Natl Acad Sci USA 79:5006–5010
Perl-Treves R, Galun E (1985) The Cucumis plastome: physical map, intragenic variation and phylogenetic relationships. Theor Appl Genet 71:417–429
Perl-Treves R, Zamir D, Navot N, Galun E (1985) Phylogeny of Cucumis based on isozyme variability and its comparison with plastome phylogeny. Theor Appl Genet 71:430–436
Phillips A (1985) Restriction map and clone bank of tomato plastid DNA. Curr Genet 10:147–152
Ruether U (1982) pUR250 allows rapid chemical sequencing of both DNA strands of its inserts. Nucleic Acids Res 10:5765–5772
Rusche JR, Howard-Flanders P (1985) Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase. Nucleic Acids Res 13:1997–2008
Shinozaki K, Ohme T, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chungwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986 a) The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO J 5:2043–2049
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayasha T, Zaita N, Chungwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kata A, Tohdoh N, Shimada H, Sugiura M (1986 b) The complete nucleotide sequence of the tobacco chloroplast genome. Plant Mol Biol Rep 4:111–175
Smillie RM, Hetherington SE, Ochoa C, Malagamba P (1983) Tolerance of wild potato species from different altitudes to cold and heat. Planta 159:112–118
Summers J (1975) Physical map of Polyoma viral DNA fragments produced by cleavage with a restriction enzyme from Haemophilus aegypticus: endonuclease R. HaeIII. J Virol 15:946–953
Zhu YX, Duvall EJ, Lovett PS, Kung SD (1982) Nicotiana chloroplast genome. V. Construction, mapping and expression of clone library of N. otophora chloroplast DNA. Mol Gen Genet 187:61–66
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Heinhorst, S., Gannon, G.C., Galun, E. et al. Clone bank and physical and genetic map of potato chloroplast DNA. Theoret. Appl. Genetics 75, 244–251 (1988). https://doi.org/10.1007/BF00303960
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DOI: https://doi.org/10.1007/BF00303960