Summary
We have detected and isolated a macromolecular constituent in normal human urine possessing calcium crystal growth inhibitory activity. The purification procedure consisted of two anion exchange chromatographies and one affinity chromatography. The crystal growth inhibitor was found to be heterogeneous in net charge as well as in size. It has not been identified. It is not an uronic acid-containing glycosaminoglycan, hitherto presumed to be responsible for the inhibitory activity. Whether an urinary fragment of inter-α-trypsin inhibitor is responsible has yet to be resolved.
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Blumenkrantz N, Asboe-Hansen G (1973) New method for quantitative determination of uronic acids. Anal Biochem 54:484
Bowyer RC, Brockis JG, McCulloch RK (1979) Glycosaminoglycans as inhibitors of calcium oxalate crystal growth and aggregation. Clin Chim Acta 95:23
Drach GW (1986) Urinary lithiasis. In: Walsh PC, Gittes RF, Perlmutter AD, Stamey TA (eds) Campbell's Urology, vol 1, chapt 25. Saunders, Philadelphia, p 1094
Edyvane KA, Ryall RL, Marshall VR (1985) The effect of saline bladder washings on calcium oxalate crystal growth and aggregation. J Urol 134:158
Felix R, Monod A, Broge L, Hansen NM, Fleisch H (1977) Aggregation of calcium oxalate crystals: effect of urine and various inhibitors. Urol Res 5:21
Fleisch H (1978) Inhibitors and promotors of stone formation. Kidney Int 13:361
Gardner GL, Doremus RH (1978) Crystal growth inhibitors in human urine. Effect on calcium oxalate kinetics. Invest Urol 15:478
Gill WB, Karesh JW, Garsin L, Roma MJ (1977) Inhibitory effects of urinary macromolecules on the crystallization of calcium oxalate. Invest Urol 15:95
Harboe NMG, Just Svendsen P (1983) Fused rocket immunoelectrophoresis. Scand J Immunol 17 [Suppl 10]:107
Hennessey PT, Hurst RE, Hemstreet GP III, Cutter G (1981) Urinary glycosaminoglycan excretion as a biochemical marker in patients with bladder carcinoma. Cancer Res 41:3868
Ito H, Coe FL (1977) Acidic peptide and polyribonucleotide crystal growth inhibitors in human urine. Am J Physiol 233:F455
Jourdian GW, Wolfman M, Sarber R, Distler J (1979) A specific, sensitive method for the determination of hyaluronate. Anal Biochem 96:474
Juang R-H, Chang Y-D, Sung H-Y, Su J-C (1984) Oven-drying method for polyacrylamide gel slab packed in cellophane sandwich. Anal Biochem 141:348
Ligabue A, Fini M, Robertson WG (1979) Influence of urine on “in vitro” crystallization rate of calcium oxalate: determination of inhibitory activity by a [14C] oxalate technique. Clin Chim Acta 98:39
Lopez M, Nakagawa Y, Coe FL, Tsai C, Michael AF, Scheinman JI (1986) Immunochemistry of urinary calcium oxalate crystal growth inhibitor (CGI). Kidney Int 29:829
Martin X, Opgenorth TJ, Werness PG, Rundquist RT, Romero JC, Smith LH (1985) Bladder secretion of inhibitors of calcium crystal growth. Kidney Int 28:636
Meyer JL, Smith LH (1975) Growth of calcium oxalate crystals. II. Inhibition by natural urinary crystal growth inhibitors. Invest Urol 13:36
Morrissey J (1981) Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem 117:307
Nakagawa Y, Margolis HC, Yokoyama S, Kézdy FJ, Kaiser ET, Coe FL (1981) Purification and characterization of a calcium oxalate monohydrate crystal growth inhibitor from human kidney tissue culture medium J Biol Chem 256:3936
Nakagawa Y, Abram V, Kézdy FJ, Kaiser ET, Coe FL (1983) Purification and characterization of the principal inhibitor of calcium oxalate monohydrate crystal growth in human urine. J Biol Chem 258:12594
Poulsen JH, Cramers MK (1982) Dermatan sulphate in urine reflects the extent of skin affection in proriasis. Clin Chim Acta 126:119
Robertson WG, Peacock M, Nordin BEC (1973) Inhibitors of the growth and aggregation of calcium oxalate crystals in vitro. Clin Chim Acta 43:31
Robertson WG, Scurr DS, Bridge CM (1981) Factors influencing the crystallisation of calcium oxalate in urine-critique. J Crystal Growth 53:182
Ryall RL, Harnett RM, Marshall VR (1981) The effect of urine, pyrophosphate, citrate, magnesium, and glycosaminoglycans on the growth and aggregation of calcium oxalate crystals in vitro. Clin Chim Acta 112:349
Saito H, Yamagata T, Suzuki S (1968) Enzymatic method for the determination of small quantities of isomeric chondroitin sulfates. J Biol Chem 243:1536
Sallis JD, Lumley MF (1979) On the possible role of glycosaminoglycans as natural inhibitors of calcium oxalate stones. Invest Urol 16:296
Staun M, Norén O, Sjöström H (1984) Ca2+-binding protein from human kidney. Purification and properties. Biochem J 217:229
Stenman U-H, Pesonen K, Huhtala M-L (1982) Rapid concentration of urinary peptides and proteins. Anal Biochem 123:291
Tiselius H-G, Fornander A-M (1981) Evaluation of a routine method for determination of calcium oxalate crystal growth inhibition in diluted urine samples. Clin Chem 27:565
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Sørensen, S., Hansen, K., Bak, S. et al. An unidentified macromolecular inhibitory constituent of calcium oxalate crystal growth in human urine. Urol. Res. 18, 373–379 (1990). https://doi.org/10.1007/BF00297368
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DOI: https://doi.org/10.1007/BF00297368