Summary
An ethionine resistant mutant of Candida utilis was found to maintain an expanded intracellular pool of free l-methionine in batch and continuous cultures. During glucose-limited growth in mineral salts medium in a continuous fermenter, the free l-methionine pool of the mutant was 40–80% higher than in batch cultures, and varied in the range of 25–30 μmoles/g dry cells (3.7–4.5 mg/g dry cells).
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Dunyak, S.A., Cook, T.M. Continuous fermenter growth of a methionine-overproducing mutant of Candida utilis . Appl Microbiol Biotechnol 21, 182–186 (1985). https://doi.org/10.1007/BF00295117
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DOI: https://doi.org/10.1007/BF00295117