Abstract
We have selected two recessive mutants of tomato with slightly longer hypocotyls than the wild type, one under low fluence rate (3 μmol/m2/s) red light (R) and the other under low fluence rate blue light. These two mutants were shown to be allelic and further analysis revealed that hypocotyl growth was totally insensitive to far-red light (FR). We propose the gene symbol fri (far-red light insensitive) for this locus and have mapped it on chromosome 10. Immunochemically detectable phytochrome A polypeptide is essentially absent in the fri mutants as is the bulk spectrophotometrically detectable labile phytochrome pool in etiolated seedlings. A phytochrome B-like polypeptide is present in normal amounts and a small stable phytochrome pool can be readily detected by spectrophotometry in the fri mutants. Inhibition of hypocotyl growth by a R pulse given every 4 h is quantitatively similar in the fri mutants and wild type and the effect is to a large extent reversible if R pulses are followed immediately by a FR pulse. After 7 days in darkness, both fri mutants and the wild type become green on transfer to white light, but after 7 days in FR, the wild-type seedlings that have expanded their cotyledons lose their capacity to green in white light, while the fri mutants de-etiolate. Adult plants of the fri mutants show retarded growth and are prone to wilting, but exhibit a normal elongation response to FR given at the end of the daily photoperiod. The inhibition of seed germination by continuous FR exhibited by the wild type is normal in the fri mutants. It is proposed that these fri mutants are putative phytochrome A mutants which have normal pools of other phytochromes.
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Adamse P, Jaspers PAPM, Bakker JA, Wesselius JC, Heeringa GH, Kendrick RE, Koornneef M (1988) Photophysiology of a tomato deficient in labile phytochrome. Plant Physiol 133:436–440
Ahmad M, Cashmore AR (1993) HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor. Nature 366:162–166
Dehesh K, Franci C, Parks BM, Seeley KA, Short TW Tepperman JM, Quail PH (1993) Arabidopsis HY8 locus encodes phytochrome A. Plant Cell 5:1081–1088
Devlin PF, Rood SB, Somers DE, Quail PH, Whitelam GC (1992) Photophysiology of the elongated internode (ein) mutant of Brassica rapa. Plant Physiol 100:1442–1447
Hauser B, Cordonnier-Pratt M-M, Pratt LH (1994) Differential expression of five phytochrome genes in tomato (Lycopersicon esculentum Mill.). Plant Physiol 105 (suppl):72
Hiscox JD, Israelstam GF (1979) A method for extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1332–1334
Koornneef M, Kendrick RE (1994) Photomorphogenic mutants of higher plants. In: Kendrick RE, Kronenberg GHM (eds) Photomorphogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp 601–628
Koornneef M, Rollf E, Spruit CJP (1980) Genetic control of lightinhibited hypocotyl elongation in Arabidopsis thaliana (L.) Heynh. Z Pflanzenphysiol 100:147–160
Koornneef M, Cone JW Dekens RG, O'Herne-Robers EG, Spruit CJP, Kendrick RE (1985) Photomorphogenic responses of long hypocotyl mutants of tomato. Plant Physiol 120:153–165
Koornneef M, Bosma TDG, Hanhart CJ, Van der Veen JH, Zeevaart JAD (1990) The isolation and characterization of gibberellin-deficient mutants in tomato. Theor Appl Genet 80:852–857
Kraepiel Y, Julien M, Cordonnier-Pratt M-M, Pratt L (1994) Identification of two loci involved in phytochrome expression in Nicotiana plumbaginifolia and lethality of the corresponding double mutant. Mol Gen Genet 242:559–565
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lichtenthaler HK, Wellbrun AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11:591–592
Liscum E, Hangarter RP (1991) Arabidopsis mutants lacking blue light-dependent inhibition of hypocotyl elongation. Plant Cell 3:685–694
Liscum E, Young JC, Poff KL, Hangarter RP (1992) Genetic separation of phototropism and blue light inhibition of stem elongation. Plant Physiol 100:267–271
López-Juez E, Nagatani A, Tomizawa K-I, Deak M, Kern R, Kendrick RE, Furuya M (1992) The cucumber long hypocotyl mutant lacks a light-stable PHYB-like phytochrome. Plant Cell 4:241–251
Mancinelli AL (1994) The physiology of phytochrome action. In: Kendrick RE, Kronenberg GHM (eds) Photomorphogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp 211–269
Mohr H (1994) Coaction between pigment systems. In: Kronenberg GHM (eds) Photomorphogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp 353–373
Nagatani A, Yamamoto KT, Fukomoto T, Yamashita A (1985) Production and characterization of monoclonal antibodies which distinguish different surface structures of pea (Pisum sativum cv. Alaska) phytochrome. Plant Cell Physiol 25:1059–1068
Nagatani A, Reed JW, Chory J (1993) Isolation and initial characterization of Arabidopsis mutants that are deficient in phytochrome A. Plant Physiol 102:269–277
Parks BM, Quail PH (1991) Phytochrome-deficient hy1 and hy2 long hypocotyl mutants of Arabidopsis are defective in phytochrome chromophore biosynthesis. Plant Cell 3:1177–1186
Parks BM, Quail PH (1993) hy8, a new class of Arabidopsis long hypocotyl mutants deficient in functional phytochrome A. Plant Cell 5:39–48
Quail PH (1994) Phytochrome genes and their expression. In: Kendrick RE, Kronenberg GHM (eds) Photomorphogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp 71–103
Reed JW, Nagpal P, Poole DS, Furuya M, Chory J (1993) Mutations in the gene for red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development. Plant Cell 5:147–157
Sharma R, López-Juez E, Nagatani A, Furuya M (1993) Identification of photo-inactive phytochrome A in etiolated seedlings and photo-active phytochrome B in green leaves of the aurea mutant of tomato. Plant J 4:1035–1042
Sharrock RA, Parks BM, Koornneef M, Quail PH (1988) Molecular analysis of the phytochrome deficiency in an aurea mutant of tomato. Mol Gen Genet 213:9–14
Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JOINMAP. Plant J 3:739–744
Tanksley SD (1993) Linkage map of tomato (Lycopersicon esculenturn). In: O'Brien SJ (ed) Genetic maps. Cold Spring Harbor Laboratory Press, New York, pp 6–39
Whitelam GC, Johnson E, Peng J, Carol P, Anderson ML, Cowl JS, Harberd NP (1993) Phytochrome A null mutants of Arabidopsis display a wild-type phenotype in white light. Plant Cell 5:757–768
Young JC, Liscum E, Hangarter RP (1992) Spectral-dependence of light-inhibited hypocotyl elongation in photomorphogenic mutants of Arabidopsis: evidence for a UV-A photosensor. Planta 188:106–114
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Communicated by A. Kondorosi
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van Tuinen, A., Kerckhoffs, L.H.J., Nagatani, A. et al. Far-red light-insensitive, phytochrome A-deficient mutants of tomato. Molec. Gen. Genet. 246, 133–141 (1995). https://doi.org/10.1007/BF00294675
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DOI: https://doi.org/10.1007/BF00294675