Summary
The in situ spatial distribution of nucleolus-organizing-region (NOR) bearing chromosomes in relation to the inactive X chromosome was studied during interphase in human fibroblasts. The respective positions of these chromosomes were examined in 30 growing and 32 resting fibroblasts from reconstructed nuclei, using nucleoli and the Barr body as ultrastructural markers. Experimental values for the distance between the nucleoli and the Barr body were estimated by their coefficient of closeness and compared to the uniform distribution. The following results were obtained: (1) the distribution patterns for the two populations of nuclei were similar, (2) the distribution of the NOR-bearing chromosomes in relation to the inactive X chromosome varied and differed significantly from a uniform distribution, and (3) in many cases the Barr body was observed to be in a juxta-nucleolar position. The internal distribution revealed by this study is compared with the data in the literature, especially with the conflicting data obtained by other methods used to determine the interphase arrangement of chromosomes. The relationship between interphase and metaphase arrangements such as can be deduced with these methods, is discussed in relation to the mechanisms of the formation of metaphase plates or chromatid translocations.
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References
Anteunis A, Pouchelet M (1978) Nucleic acid staining with 3,3′-diaminobenzidine: an improved DAB oxidation procedure. Stain Technol 53:241–242
Avivi L, Feldman M (1980) Arrangement of chromosomes in the interphase nucleus of plants. Hum Genet 55:281–295
Barr ML (1966) The significance of the sex chromatin. Int Rev Cytol 19:35–95
Barr ML, Bertram LF (1949) A morphological distinction between neurones of the male and female and the behaviour of the nucleolar satellite during accelerated nucleoprotein synthesis. Nature 163:676–677
Barton DE, David FN, Merrington M (1964) The positions of the sex chromosomes in the human cell mitosis. Ann Hum Genet 28:123–128
Bennett MD (1982) Nucleotypic basis of the spatial ordering of chromosomes in eukaryotes and the implications of the order for genome evolution and phenotypic variation. In: Douer GA, Flavell RB (eds) Genome evolution. Academic Press, New York, pp 239–261
Bobrow M, Pearson PL, Collacott HEAC (1971) Paranucleolar position of the human Y chromosome in interphase nuclei. Nature 232:556–557
Bourgeois CA (1974) Distribution of mitomycin C-induced damage in human chromosomes with special reference to regions of repetitive DNA. Chromosoma 48:203–211
Bourgeois CA (1975) The analysis of spontaneous and induced damage to human chromosomes using banding techniques. M Phil University of London
Bourgeois CA, Hemon D, Beaure D'Augères C, Robineaux R, Bouteille M (1981) Kinetics of nucleolus location within the nucleus by time-lapse microcinematography. Biol Cell 40:229–232
Bourgeois CA, Hemon D, Bouteille M (1982) Changes in the nucleolus-envelope region during interphase in synchronized TG cells. J Ultrastruct Res 81:257–267
Bourgeois CA, Bard F, Bouteille M (1983) Evidence showing that the nucleolus-envelope region is located on the NOR-bearing chromosomes in Aotus trivirgatus. J Submicrosc Cytol 15:873–882
Bourgeois CA, Costagliola D, Laquierriere F, Bard F, Hemon D, Bouteille M (1984) In situ arrangement of NOR-bearing chromosomes in the interphase nucleus of Aotus trivirgatus. J Cell Sci 69:107–115
Boveri Th (1909) Die Blastomerenkerne von Ascaris megalocephala und die Theorie der Chromosomenindividualität. Arch Zellforsch 3:181–268
Brogger A, Johansen J (1972) A model for production of chromosome damage by mitomycin C. Chromosoma 38:95–104
Caspersson T, Haglund U, Lindell B, Zech L (1972) Radiation-induced non-random chromosome breakage. Exp Cell Res 75:541–543
Chandra HS, Narayana AKA, Buche VV, Hungerford DA (1972) Paripheral location of the human late X and homologous association of autosomes numbers one, two and three. J Hum Genet 61:78–83
Comings DE (1968) The rationale for an ordered arrangement of chromatin in the interphase nucleus. Ann Hum Genet 520:440–460
Comings DE (1980) Arrangement of chromatin in the nucleus. Hum Genet 53:131–143
Franke WW (1974) Structure, biochemistry and functions of the nuclear envelope. Int Rev Cytol [Suppl] 4:71–236
Gagné R, Laberge C, Tanguay R (1972) Interphase association of human “Y body” with nucleolus. Johns Hopkins Med J 130:254–274
Galperin H (1968) General distribution of the 46 chromosomes in human metaphase plates. Hum Genet 6:118–130
Galperin H (1969) Relative positions of homologous chromosomes or groups in male and female metaphase figures. Humangenetik 7:265–274
Giannelli F (1974) Autoradiography of human chromosomes. In: Yunis Y (ed) Human chromosome methodology. Academic Press, New York San Francisco London, pp 127–155
Goldgefter LI, Gakjov NY, Ganin AF, Mosolov AN (1973) Identifying Y chromosome in interphase nuclei of the human brain. Experientia 29:1428–1429
Hager HD, Schroeder-Kurth TM, Vogel F (1982) Position of chromosomes in the interphase nucleus. Hum Genet 61:342–356
Hancock R, Boulikas T (1982) Functional organization in the nucleus. Int Rev Cytol 79:165–214
Hemon D, Bourgeois CA, Bouteille M (1981) Analysis of the spatial organization of the cell: a statistical method for revealing the nonrandom location of an organelle. J Microsc 121:29–37
Hens L (1976) Chromosome distribution in a23 Chinese hamster fibroblasts. Chromosoma 57:205–217
Hens L, Kirsch-Volders M, Verschaeve L, Susanne C (1982) The central localization of the small and early replicating chromosomes in human diploid metaphase figures. Hum Genet 60:249–256
Hochn H, Martin GM (1973) Non-random arrangement of the human chromatin: topography of disomicmarkers X, Y and 1h+. Cytogenet Cell Genet 12:443–452
Hubert J Bureau J, Bouteille M (to be published) Anchorage of the nucleolus in the pore complex-lamina by a DNA-bearing structure masked in situ in rat liver nuclei. Biol Cell
Kihlman BA (1971) Molecular mechanisms of chromosome breakage and rejoining. In: Dupraw E (ed) Advances in cell and molecular biology. Academic Press, New York London pp 59–107
Kirsch-Volders M, Hens L, Susanne C, Galperin-Lemaitre H (1977) Stability of centromere center distances in normal human metaphases. Cytogenet Cell Genet 18:61–74
Kowarzyk H, Steinhaus H, Szymaniec S (1965) Arrangement of chromosomes in human cells. I. Association in metaphase figures. Bull Acad Sci [Biol] VI:321–326
Lyon MF (1962) Sex chromatin and gene action in mammalian X chromosome. Am J Hum Genet 14:135–148
Lyon MF (1972) X chromosome inactivation and developmental patterns in mammals. Biol Rev 47:1–35
Miller OJ, Mukherjee BB, Breg WR, Gamble A Van N (1963) Nonrandom distribution of chromosomes in metaphases figures from cultured human leukocytes. I. The peripheral location of the Y chromosome. Cytogenetics 2:1–14
Moore KL (1966) Sex chromatin patterns in various animals. In: Moore KL (ed) The sex chromatin. Saunders. Philadelphia London, pp 16–58
Morad M, Jonasson J, Lindsten J (1973) Distribution of mitomycin C induced breaks on human chromosomes. Hereditas 74:273–282
Natarajan AT, Ahnström G (1969) Heterochromatin and chromosome aberrations. Chromosoma 28:48–61
Ockey CH (1969) The position of chromosomes at metaphase in human fibroblasts and their DNA synthesis behavior. Chromosoma 27:308–320
Petersen GB, Therkelsen AJ (1962) Number of nucleoli in female and male human cells in tissue culture. Exp Cell Res 28:590–592
Rabl C (1885) Über Zelltheilung. Morphologisches Jahrbuch 10:214–330
Rappold GA, Cremer T, Hager HD, Davies KE, Müller CR, Yang T (1984) Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes. Hum Genet67:317–325
Rodman TC, Flehinger BJ, Squire RD (1978) Patterns of association in the human metaphase complement: ring analysis and estimation of associativity of specific chromosome regions. Hum Genet 41:19–34
Rodman TC, Flehinger BJ, Rohlf FJ (1980) Metaphase chromosome associations: Colcemid distorts the pattern. Cytogenet Cell Genet 27:98–110
Roels F, Goldfischer S (1971) Staining of nucleic acids with 3,3′-diaminobenzidine (DAB). J Histochem Cytochem 19:713–714
Schellens JPM, James J, Hoeben KA (1979) Some aspects of the fine structure of the sex chromatin body. Biol Cell 35:11–14
Sele B, Jalbert P, Van Cutsem B, Lucas M, Moriquand C (1977) Distribution of human chromosomes on the metaphase plate using banding techniques. Hum Genet 39:39–61
Stahl A (1982) The nucleolus and nucleolar chromosomes. In: Jordan EG, Cullis CA (eds) The nucleolus. Cambridge University Press, Cambridge, pp 1–24
Vogel F, Schroeder TM (1974) The internal order of the interphase nucleus. Humangenetik 25:265–297
Wollenberg C, Kiefaber MP, Zang KD (1981) The arrangement of human metaphase chromosomes with and without spindle apparatus. Int Congr Hum Genet VI:122
Wollenberg C, Kiefaber MP, Zang KD (1982) Quantitative studies on the arrangement of human metaphase chromosomes: VIII. Localization of homologous chromosomes. Hum Genet 60:239–248
Wyandt HE, Iorio RJ (1973) Human Y chromatin. III. The nucleolus. Exp Cell Res 81:468–473
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Bourgeois, C.A., Laquerriere, F., Hemon, D. et al. New data on the in situ position of the inactive X chromosome in the interphase nucleus of human fibroblasts. Hum Genet 69, 122–129 (1985). https://doi.org/10.1007/BF00293281
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DOI: https://doi.org/10.1007/BF00293281