Abstract
Normal synaptonemal complexes have been found in haploid barley meiotic prophase at stages equivalent to pachytene in diploids. Reconstructions of serially sectioned nuclei have shown that up to 60% of the haploid chromosomes may pair in either intra- or interchromosomal associations. The extent and nature of the synaptonemal complex formation suggest that the chromosome pairing is non-homologous. From the virtual absence of chiasmata in metaphase I stages of the haploids it is inferred that crossing over requires a more precise DNA alignment than is provided by synaptonemal complex formation alone.
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Bernhard, W. A.: A new staining procedure for electron microscopical cytology. J. Ultrastruct. Res. 27, 250–265 (1969)
Burnham, C. R., Stout, J. T., Weinheimer, W. H., Knowles, R, V., Phillips, R. L.: Chromosome pairing in maize. Genetics 71, 111–126 (1972)
Gassner, G.: Synaptinemal complexes in the achiasmatic spermatogenesis of Bolbe nigra Giglio-Tos (Mantoidea). Chromosoma (Berl.) 26, 22–34 (1969)
Gillies, C. B.: Reconstruction of the Neurospora crassa pachytene karyotype from serial section of synaptonemal complexes. Chromosoma (Berl.) 36, 119–130 (1972)
Gillies, C. B.: Methods for electron microscopic study of maize pachytene chromosomes. Maize Genetics Coop. New Letter 47, 25–27 (1973a)
Gillies, C. B.: Ultrastructural analysis of maize pachytene karyotypes by three dimensional reconstruction of the synaptonemal complexes. Chromosoma (Berl.) 43, 145–176 (1973b)
Jensen, C. J.: Production of monoploids in barley. Barley Genetics Newsletter 3, 23–24 (1973)
Kasha, K. J., Kao, K. N.: High frequency haploid production in barley (Hordeum vulgare L.). Nature (Lond.) 225, 874–876 (1970)
Levan, A.: Studies on the meiotic mechanism of haploid rye. Hereditas (Lund) 28, 177–211 (1942)
McClintock, B.: The association of non-homologous parts of chromosomes in the mid-prophase of meiosis in Zea mays. Z. Zellforsch. 19, 191–237 (1933)
Menzel, M. Y., Price J. M.: Fine structure of synapsed chromosomes in F1 Lycopersicon esculentum — Solanum lycopersicoides and its parents. Amer. J. Bot. 53, 1079–1086 (1966)
Moens, P. B.: Genetic and cytological effects of three desynaptic genes in the tomato. Canad. J. Genet. Cytol. 11, 857–869 (1969)
Moens, P. B.: Quantitative electron microscopy of chromosome organization at meiotic prophase. Cold Spr. Harb. Symp. quant. Biol. 38, 99–107 (1973)
Nilan, R. A., Wettstein, D. von, Eden, U.: The occurrence of Synaptinemal complexes at meiotic prophase in barley. Barley Newsletter 12, 40–41 (1969)
Rasmussen, S. W.: Studies on the development and ultrastructure of the synaptinemal complex in Drosophila melanogaster C. R. Lab. Carlsberg 39, 443–468 (1974)
Rieger, R.: Inhomologenpaarung und Meioseablauf bei haploiden Formen von Antirrhinum majus L. Chromosoma (Berl.) 9, 1–38 (1957)
Sadasivaiah, R. S., Kasha, K. J.: Meiosis in haploid barley — an interpretation of non-homologous chromosome associations. Chromosoma (Berl.) 35, 247–263 (1971)
Sarvella, P., Holmgren, J. B., Nilan, R. A.: Analysis of barley pachytene chromosomes. The Nucleus (Calcutta) 1, 183–204 (1958)
Sen, S. K.: Synaptonemal complexes in haploid Petunia and Antirrhinum sp. Naturwissenschaften 57, 550 (1970)
Sheridan, W. F., Barrnett, R. J.: Cytochemical studies on chromosome ultrastructure. J. Ultrastruct. Res. 27, 216–229 (1969)
Spurr, A. R.: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43 (1969)
Stern, H., Hotta, Y.: Biochemical controls of meiosis. Ann. Rev. Genet. 7, 37–66 (1973)
Subrahmanyam, N. C., Kasha, K. J.: Selective chromosomal elimination during haploid formation in barley following interspecific hybridization. Chromosoma (Berl.) 42, 111–125 (1973)
Ting, Y. C.: Further studies on the synaptonemal complex of haploid maize. Genetics 68, Suppl., 67 (1971)
Weber, D. F., Alexander, D. E.: Redundant segments in Zea mays detected by translocations of monoploid origin. Chromosoma (Berl.) 39, 27–42 (1972)
Westergaard, M., Wettstein, D. von: The synaptinemal complex. Ann. Rev. Genet. 6, 71–110 (1972)
Wettstein, D. von: The synaptinemal complex and four-strand crossing over. Proc. nat. Acad. Sci. (Wash.) 68, 851–855 (1971)
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Gillies, C.B. The nature and extent of synaptonemal complex formation in haploid barley. Chromosoma 48, 441–453 (1974). https://doi.org/10.1007/BF00290998
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DOI: https://doi.org/10.1007/BF00290998