Abstract
The transformation booster sequence (TBS) from Petunia hybrida enhances transformation frequencies in P. hybrida, Nicotiana tabacum and Zea mays. TBS also stimulates homologous inter- and intramolecular recombination in P. hybrida, the molecular basis for this stimulation is not known. We investigated whether TBS contains sequence elements that might contribute to the stimulation of recombination and whether its recombinogenic potential reflects a biological function of TBS. We identified a scaffold attachment region (SAR) within TBS and analysed its distribution in the genome and its homologies to other genomic sequences. A 516 by subfragment of TBS binds to the nuclear scaffold. The sequence of the TBSSAR fragment shows strong homologies to retroviral elements from plants, suggesting that TBS is an inactive derivative of a retrovirus that still promotes DNA recombination.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Allen GC, Hall GE, Childs LC, Weissinger AK, Spiker S, Thompson WF (1993) Scaffolds attachment regions increase reporter gene expression in stably transformed plant cells. Plant Cell 5:603–613
Altschul SF, Miller W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Benbow RM, Zhao J, Larson DD (1992) On the nature of origins of DNA replication in eucaryotes. BioEssays 14:661–670
Bingham PM, Zachar Z (1989) Retrotransposons and the FB transposon from Drosophila melanogaster. In: Berg DE, Howe MM (eds) Mobile DNA. American Society for Microbiology, Washington DC, pp 485–502
Boeke JD (1989) Transposable elements in Saccharomyces cerevisiae. In: Berg DE, Howe MM (eds) Mobile DNA. American Society for Microbiology, Washington DC, pp 335–374
Breyne P, Van Montagu M, Depicker A, Gheysen G (1992) Characterization of a scaffold attachment region in a DNA fragment that normalizes transgene expression in tobacco. Plant Cell 4:463–471
Buising CM, Benbow RM (1994) Molecular analysis of transgenic plants generated by microprojectile bombardment: effect of petunia transformation booster sequence. Mol Gen Genet 243:71–81
Camirand A, St Pierre B, Marineau C, Brisson N (1990) Occurrence of a copia-like transposable element in one of the introns of potato starch phosphorylase gene. Mol Gen Genet 224:33–39
Dillehay LE, Jacobson-Kram D, Williams JR (1989) DNA topoisomerases and models of sister-chromatid exchange. Mutat Res 215:15–23
Engels P (1991) Vergleichende Studien zur homologen Rekombination in Petunia hybrida cv. RL01 und Nicotiana tobacum cv. Petit Havana SRI. PhD Thesis, University of Cologne
Engels P, Meyer P (1992) Comparison of homologous recombination frequencies in somatic cells of petunia and tobacco suggest two distinct recombination pathways. Plant J 2:59–67
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Gasser SM, Laemmli UK (1986) Cohabitation of scaffold binding regions with upstream/enhancer elements of three developmentally regulated genes of D. melanogaster. Cell 46:521–530
Gasser SM, Laemmli UK (1987) A glimpse at chromosomal order. Trends Genet 3:16–22
Goodall GJ, Filipowicz W (1991) Different effects of intron nucleotide composition and secondary structure on pre-mRNA splicing in monocot and dicot plants. EMBO J 10:2635–2644
Grandbastien MA (1992) Retroelements in higher plants. Trends Genet 8:103–108
Grandbastien MA, Spielmann A, Caboche M (1989) Tnt 1, a mobile retroviral-like transposable element of tobacco isolated by plant cell genetics. Nature 337:376–380
Harberd NP, Flavell RB, Thompson RD (1987) Identification of a transposon-like element in a Glu-1 allele of wheat. Mol Gen Genet 209:376–380
Hein T, Prezewozni T, Schieder O (1983) Culture and selection of somatic hybrids using an auxotrophic cell line. Theor Appl Genet 64:119–122
Hirochika H (1993) Activation of tobacco retrotransposons during tissue culture. EMBO J 12:2521–2528
Hirochika H, Hirochika R (1993) Ty1-copia group retrotransposons as ubiquitous components of plant genomes. Jpn J Genet 68:35–46
Hutchison III CA, Hardies SC, Loeb DD, Shehee WR, Edgell MH (1989) LINES and related retroposons: long interspersed repeated sequences in the eucaryotic genome. In: Berg DE, Howe MM (eds) Mobile DNA.American Society for Microbiology, Washington DC, pp 593–617
Kartzke S, Saedler H, Meyer P (1990) Molecular analysis of transgenic plants derived from transformations of protoplasts at various stages of the cell cycle. Plant Sci 67:63–72
Kricker MC, Drake JW, Radman M (1992) Duplication-targeted DNA methylation and mutagenesis in the evolution of eucaryotic chromosomes. Proc Natl Acad Sci USA 89:1075–1079
Landy A (1989) Dynamic, structural and regulatory aspects of lambda site-specific recombination. Annu Rev Biochem 58:913–949
Meyer P, Kartzke S, Niedenhof I, Heidmann I, Bussmann K, Saedler H (1988) A genomic DNA segment from Petunia hybrida leads to inceased transformation frequencies and simple integration patterns. Proc Natl Acad Sci USA 85:8568–8572
Mirkovitch J, Mirault ME, Laemmli UK (1984) Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold. Cell 39:223–232
Miura-Masuda A, Ikeda H (1990) The DNA gyrase of Escherichia coli participates in the formation of a spontaneous deletion by recA-independent recombination in vivo. Mol Gen Genet 220:345–352
Moore G, Lucas H, Batty N, Flavell R (1991) A family of retrotransposons and associated genomic variation in wheat. Genomics 10:461–468
Phi-Van L, Von Kries JP, Ostertag W, Strätling WH (1990) The chicken lysozyme 5' matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genes. Mol Cell Biol 10:2302–2307
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Sander M, Hsieh T (1985) Drosophila topoisomerase II double strand DNA cleavage: analysis of DNA homology at the cleavage site. Nucleic Acids Res 13:1057–1071
Schöfll F, Schröder G, Kliem M, Rieping M (1993) An SAR sequence containing 395 by DNA fragment mediates enhanced, gene-dosage-correlated expression of a chimeric heat shock gene in transgenic tobacco plants. Transgenic Res 2:93–100
Schwarz-Sommer Z, Leclercq L, Göbel E, Saedler H (1987) Cin-4, an insert altering the structure of the Al gene in Zea mays, exhibits properties of nonviral retrotransposons. EMBO J 6:3873–3880
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–507
Stief A, Winter DM, Stratling WH, Sippel AE (1989) A nuclear DNA attachment element mediates elevated and position independent gene activity. Nature 341:343–345
Stotz G, Den Vlaming P, Wiering H, Schramm AW, Forkmann G (1985) Genetic and biochemical studies on flavonoid 3′-hydroxylation in flowers of Petunia hybrida. Theor Appl Genet 70:300–305
Umek RM, Kowalski D (1988) The ease of DNA unwinding as a determinant of initiation at yeast replication origins. Cell 52:559–567
Voytas DF, Cummings MP, Konieczny A, Ausubel FM, Rodermel SR (1992) Copia-like retrotransposons are ubiquitous among plants. Proc Natl Acad Sci USA 89:7124–7128
Werr W, Lörz H (1986) Transient gene expression in a Gramineae cell line. Mol Gen Genet 202:471–475
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Galliano, H., Müller, A.E., Lucht, J.M. et al. The transformation booster sequence from Petunia hybrida is a retrotransposon derivative that binds to the nuclear scaffold. Molec. Gen. Genet. 247, 614–622 (1995). https://doi.org/10.1007/BF00290353
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00290353