Summary
A comparative analysis of the Vicia faba mitochondrial genome in whole plants and in longterm suspension culture has been conducted. Restriction fragment patterns of the mtDNA isolated from these two sources were notably different. Electronmicroscopic analysis also revealed significant differences. Large circular mtDNA patterns shifted from a 37–80 kb subpopulation, which was predominant in whole plants, to 18–34 kb subpopulations although in both classes notable quantities of circular molecules of 80 to 120 kb and more were also found. Both in whole plant and suspension culture cells very large circular DNAs were observed. Some of them had lengths nearly 290 kb and could be considered as evidence of the existence of master chromosomes. The minicircular DNA population was also altered. In the suspension culture we observed a notable increase of percentage of minicircles with sizes near 1 kb. Simultaneously, the percentage of minicircles with sizes near 3.5–10 kb significantly increased in suspension culture cells. In addition, a new peak (10–12 kb) of minicircles appeared. Copy number alterations for some sequences homologous to CCC1A, CCC1B and CCC2 (Negruk et al. 1982, 1985) were shown. Southern hybridization revealed the existence of a family of minicircles having sizes 1.4–2 kb with predominance of CCC1A, CCC1B and CCC2. The copy numbers of CCC1B and some minor minicircles was changed in the suspension culture when compared with the whole plants.
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References
Bendich AJ (1982) Plant mitochondrial DNA: the last frontier. In: Slonimski P, Borst P, Attardi G (eds) Mitochondrial genes. Cold Spring Harbor, pp 477–500
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Brennicke A, Blanz P (1982) Circular mitochondrial DNA species from Oenothera with unique sequences. Mol Gen Genet 187:461–466
Dale RMK (1981) Sequence homology among different size classes of plant mtDNAs. Proc Natl Acad Sci USA 78:4453–4457
Dale RMK, Duesing JH, Keen D (1981) Supercoiled mitochondrial DNAs from plant tissue culture cells. Nucleic Acids Res 9:4583–4593
Davis RW, Simon H, Davidson N (1971) Electron microscope heteroduplex methods for mapping regions of base-sequence homology in nucleic acids. In: Grossman L, Moldave K (eds) Methods in enzymology, vol 21D. Academic Press, New York London, pp 413–428
Eisner GI, Negruk VI, Cherny DI, Alexandrov AA, Shemyakin MF, Butenko RG (1984) Circular DNAs in mitochondria of Vicia faba. FEBS Lett 172:67–69
Fontarnau A, Hernandes-Jago J (1982) Characterization of mitochondrial DNA in citrus. Plant Physiol 70:1678–1682
Goblet JP, Boutry M, Duc G, Briquet M (1983) Mitochondrial plasmid-like molecules in fertile and sterile Vicia faba L. Plant Mol Biol 2:305–309
Goblet JP, Flamand M-C, Briquet M (1985) A mitochondrial plasmid specifically associated with male sterility and its relation with other mitochondrial plasmids in Vicia faba. Curr Genet 9:423–426
Gross SR, Hsieh T, Levine PH (1984) Intramolecular recombination as a source of mitochondrial chromosome heteromorphism in Neurospora. Cell 38:233–239
Kemble RJ, Shepard JF (1984) Cytoplasmic DNA variation in a potato protoclonal population. Theor Appl Genet 69:211–216
Kislev N, Rubinstein I (1980) Utility of ethidium bromide in the extraction from whole plants of high molecular weight maize DNA. Plant Physiol 66:1140–1143
Leaver CJ, Gray MW (1982) Mitochondrial genome organization and expression in higher plants. Ann Rev Plant Physiol 33:373–402
Levings III CS, Shah DM, Hu WWL, Pring DR, Timothy DH (1979) Molecular heterogeneity among mitochondrial DNAs from different maize cytoplasms. In: Cummings DJ, Borst P, Dawid J, Weissman SM, Fox CF (eds) Extrachromosomal DNA. Academic Press, New York, pp 3–73
Lonsdale DM (1984) A review of structure and organization of the mitochondrial genome of higher plants. Plant Mol Biol 3:202–206
Lonsdale DM, Hodge TP, Fauron CM-R (1984) The physical map and organization of the mitochondrial genome from the fertile cytoplasm of maize. Nucleic Acids Res 12:9249–9261
Lonsdale DM, Hodge TP, Fauron CM-R, Flavell RB (1983) A predicted structure for the mitochondrial genome from the fertile cytoplasm of maize. In: Goldberg RB (ed) UCLA Symp Mol Cell Biol, New Ser, vol 12. Alan R Liss, New York, pp 21–38
Maniatis T, Fritsch EF, Saulbrook J (1982) A laboratory manual molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor
McNay JW, Chourey DS, Pring DR (1984) Molecular analysis of genomic stability of mitochondrial DNA in tissue cultured cells of maize. Theor Appl Genet 67:433–437
Mitchel JP, Gildow FE (1975) The initiation and maintenance of Vicia faba tissue cultures. Physiol Plant 34:250–253
Negruk VI, Cherny DI, Nikiforova ID, Alexandrov AA, Butenko RG (1982) Isolation and characterization of minicircular DNAs found in mitochondria fraction of Vicia faba. FEBS Lett 142:115–117
Negruk VI, Goncharova NP, Gelnin LG, Mardamshin AG (1985) Molecular hybridization analysis of cloned Vicia faba minicircular DNA sequences. Mol Gen Genet 198:486–490
Palmer JD, Shields CR (1984) Tripartie structure of the Brassica campestris mitochondrial genome. Nature 307:437–440
Quetier E, Vedel F (1977) Heterogenous population of mitochondrial DNA molecules in higher plants. Nature 268:365–368
Schenk RU, Hildebrandt R (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:250–253
Sparks RBJ, Dale RMK (1980) Characterization of 3H-labeled supercoiled mitochondrial DNA from tobacco suspension culture cells. Mol Gen Genet 180:351–355
Spruill WM Jr, Levings III CS, Sederoff RR (1980) Recombinant DNA analysis indicates that the multiple chromosomes of maize mitochondria contain different sequences. Dev Genet 1:363–378
Synenki RM, Levings III CS, Shah DM (1978) Physicochemical characterization of mitochondrial DNA from soybean. Plant Physiol 61:460–464
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Communicated by Yu. Gleba
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Negruk, V.I., Eisner, G.I., Redichkina, T.D. et al. Diversity of Vicia faba circular mtDNA in whole plants and suspension cultures. Theoret. Appl. Genetics 72, 541–547 (1986). https://doi.org/10.1007/BF00289538
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DOI: https://doi.org/10.1007/BF00289538