Summary
Two plant tumors (crown galls and hairy roots) were experimentally provoked on potato cv. ‘Désirée’ by oncogenic strains of Agrobacterium tumefaciens and A. rhizogenes. A marked shift in the expression of some organ-specific genes occurred in crown galls derived from the central zone of tubers: two novel isozyme genes (Est-B and Pox-E) were expressed, two others (Est-C and Pox-F) were suppressed and the remaining ones were maintained in the original state. When the starting tissue was the stem segment, a smaller shift occurred, namely the activation of Adh-A and the suppression of Pox-F. In all cases, the isozyme profiles characterizing all crown galls, whatever their origin, were identical. Under normal aeration conditions, Adh-A was not expressed in either tumoral or non-tumoral roots. However, under the relative anaerobic conditions of in vitro cultures, a difference existed between both types of roots: Adh-A was expressed in normal but not in tumoral roots. This means that hairy roots can tolerate higher levels of anaerobiosis without giving rise to an anaerobic response. For the remaining isozymes, no alteration occurred in either organized (hairy root) or unorganized (crown gall) tumors, as compared to the corresponding non-tumoral tissues (normal root and callus, respectively).
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Anand VK, Heberlein GT (1977) Crown gall tumorigenesis in potato tuber tissue. Am J Bot 64:153–158
Freeling M (1973) Simultaneous induction by anaerobiosis or 2–4 D of multiple enzymes specified by two unlinked genes: differential Adh1–Adh2 expression in maize. Mol Gen Genet 127:215–227
Gaspard T, Penel C, Tran Thanh Van K, Greppin H (1979) Des isoperoxydases comme marqueurs de la différenciation cellulaire et du développement chez les végetaux. In: Tavlitzki J, Tran Thanh Van K (eds) Xeme rencontre de meribel sur la differenciation cellulaire. CNRS, pp 175–193
Hageman RH, Flesher D (1960) The effect of an anaerobic environment on the activity of alcohol dehydrogenase and other enzymes of corn seedlings. Arch Biochem Biophys 87:203
Lindahl R, Evces S, Sheng W (1982) Expression of the tumor aldehyde dehydrogenase phenotype during 2-acetylaminofluorene-induced hepatocarcinogenesis. Cancer Res 42:577–582
Mäder M, Munch P, Bopp M (1975) Regulation und Bedeutung der Peroxidase-Musteränderungen in sproßdifferenzierenden Kalluskulturen von N. tabacum L. Planta 123:257–265
Martínez-Zapater JM (1983) Variabilidad de aloenzimas y relaciones filogenéticas en la patata cultivada (Solanum tuberosum L.) y especies relacionadas. PhD Thesis, Universidad Autónoma de Madrid, Madrid
Martínez-Zapater JM, Oliver JL (1984) Genetic analysis of isozyme loci in tetraploid potatoes (Solanum tuberosum L.). Genetics 108:669–679
Martínez-Zapater JM, Oliver JL (1985) Isozyme gene duplication in diploid and tetraploid potatoes. Theor Appl Genet 70:172–177
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nester EW, Gordon MP, Amasino RM, Yanofsky MF (1984) Crown gall: a molecular and physiological analysis. Annu Rev Plant Physiol 35:387–413
Oliver JL, Martínez-Zapater JM (1984) Allozyme variability and phylogenetic relationships in the cultivated potato. Plant Syst Evol 148:1–18
Oliver JL, Martínez-Zapater JM (1985) A genetic classification of potato cultivars based on allozyme patterns. Theor Appl Genet 69:305–311
Rick CM, Zobel RW, Fobes FJ (1974) Four peroxidase loci in red-fruited tomato species: Genetics and geographic distribution. Proc Natl Acad Sci USA 71:835–839
Schell J, Van Montagu M, Holsters M, Zambriski P, Joos H, Inze D, Herrera-Estrella L, Depicker A, De Block M, Caplan A, Dhaese P, Van Haute E, Hernalsteens JP, De Greeve H, Leemans J, Deblaere R, Willmitzer L, Schroder J, Otten L (1984) Ti plasmids as experimental gene vector for plants. In: Arber W, Illmensee K, Peacock WJ, Starlinger P (eds) Genetic manipulation: impact on man and society. The ICSU Press, Cambridge University Press, Cambridge, pp 87–102
Selander RK, Smith MH, Yang SY, Johnson WE, Gentry JB (1971) Biochemical polymorphism and systematics in the genus Peromyscus. 1. Variation in the old-field mouse (Peromyscuspolionotus). Univ Tex Publ 7103:49–90
Staub JE, Kuhns LJ, Grun P, May B (1984) Genetic basis for isozyme variation for alkaline phosphatase and glucosephosphate isomerase in Solanum. Theor Appl Genet 67:505–513
Stigbrand JL, Millán JL, Fishman W (1982) The genetics basis of alkaline phosphatase isozyme expression. In: Ratazzi MC, Scandalios JG, Whitt GS (eds) Isozymes: current topics in biological and medical research, vol 6. Alan R Liss, New York, pp 93–117
Tepfer D (1984) Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell 37:959–967
Author information
Authors and Affiliations
Additional information
Communicated by J. MacKey
Rights and permissions
About this article
Cite this article
Oliver, J.L. Isozyme gene expression in potato tumors incited by Agrobacterium . Theoret. Appl. Genetics 72, 373–376 (1986). https://doi.org/10.1007/BF00288575
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00288575