Summary
We present a novel approach to the efficient cloning of telomere-associated sequences and demonstrate its application to the cloning and mapping of these sequences from barley. The method is a modification of the Vectorette PCR technique and allows specific amplification and cloning of subtelomeric sequences. Telomere-associated sequences isolated from barley include hypervariable, repetitive sequences. Polymorphisms detected by subtelomeric markers behaved as Mendelian factors and were mapped to the most distal positions of barley chromosomes 1, 2, and 4.
Article PDF
Avoid common mistakes on your manuscript.
References
Arnold C, Hodgson IJ (1991) Vectorette PCR: A novel approach to genomic walking. PCR Meth Appl 1:39–42
Belostotsky DA, Ananiev EV (1990) Characterization of relic DNA from barley genome. Theor Appl Genet 80:374–380
Bennet MD, Smith JB (1976) Nuclear DNA amounts in angiosperms. Phil Trans R Soc London (Biol) 274:227–274
Blackburn EH (1991 a) Structure and function of telomeres. Nature 350:569–573
Blackburn EH (1991 b) Telomeres. Trends Biochem Sci 16:378–381
Broun P, Ganal MW, Tanksley SD (1992) Telomeric arrays display high levels of heritable polymorphism among closely related plant varieties. Proc Natl Acad Sci USA 89:1354–1357
Chan CSM, Tye B-K (1983) Organization of DNA sequences and replication origins at yeast telomeres. Cell 33:563–573
Cooke HJ, Brown WRA, Rappold GA (1985) Hypervariable telomeric sequences from the human sex chromosomes are pseudoautosomal. Nature 317:687–692
Corcoran LM, Thompson JK, Walliker D, Kemp DJ (1988) Homologous recombination within subtelomeric repeat sequences generates chromosome size polymorphisms in P. falciparum. Cell 53:807–813
Cross SH, Allshire RC, McKay SJ, McGill NI, Cooke HJ (1989) Cloning of human telomeres by complementation in yeast. Nature 338:771–774
Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
Ganal MW, Lapitan NLW, Tanksley SD (1991) A molecular and cytogenetic survey of major repeated DNA sequences in tomato (Lycopersion esculentum). Plant Cell 3:87–94
Kleinhofs A (1992) The NABGMP mapping progress report. Barley Genet Newsletter (in press)
Kleinhofs A, Chao S, Sharp PJ (1988) Mapping of nitrate reductase genes in barley and wheat. In: Miller TE, Koebner RMD (eds) Proc 7th lnt Wheat Genet Symp Vol 1, Bath Press, Bath, pp 541–546
Kleinhofs A, Kilian A (1992) RFLP maps of barley. In: Phillips RL, Vasil IK (eds) DNA-based markers in plants. Advances in cellular and molecular biology of plants, vol. 1, Kluwer Academic Publishers, Dordrecht, (in press)
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
de Lange T, Shiue L, Myers RM, Cox DR, Naylor SL, Killery AM, Varmus HE (1990) Structure and variability of human chromosome ends. Mol Cell Biol 10:518–527
Murray V (1989) Improved double-stranded DNA sequencing using the linear polymerase chain reaction. Nucleic Acids Res 17:8889
Petracek ME, Lefebvre PA, Silflow CD, Berman J (1990) Chlamydomonas telomere sequences are A + T-rich but contain three consecutive G · C base pairs. Proc Natl Acad Sci USA 87:8222–8226
Richards EJ, Ausubel FM (1988) Isolation of a higher eukaryotic telomere from Arabidopsis thaliana. Cell 53:127–136
Riley J, Butler R, Ogilvie D, Finniear R, Jenner D, Powell S, Anand R, Smith JC, Markham AF (1990) A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones. Nucleic Acids Res 18:2887–2890
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning; a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Schwarzacher T, Heslop-Harrison JS (1991) In situ hybridization to plant telomeres using synthetic oligomers. Genome 34:317–323
Weber B, Collins C, Robbins C, Magenis RE, Delaney AD, Gray JW, Hayden MR (1990) Characterization and organization of DNA sequences adjacent to the human telomere-associated repeat (TTAGGG)n. Nucleic Acids Res 18:3353–3361
Zakian AW (1989) Structure and function of telomeres. Annu Rev Genet 23:579–604
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Rights and permissions
About this article
Cite this article
Kilian, A., Kleinhofs, A. Cloning and mapping of telomere-associated sequences from Hordeum vulgare L.. Molec. Gen. Genet. 235, 153–156 (1992). https://doi.org/10.1007/BF00286193
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00286193