Abstract
The structure, copy number and chromosomal location of arrays of four families of highly repeated sequences have been investigated in representative species of the genus Secale. The four unrelated families, previously characterised in Secale cereale, have repeating units of 480, 610, 630 and 120 base pairs respectively. The following general conclusions can be drawn in addition to detailed knowledge of the sequence content of heterochromatin in each accession studied: (1) Every species is unique in its complement or chromosomal distribution or both of the four highly repeated sequence families. S. montanum and S. cereale accessions studied here show the same complement of repeated sequences, but they differ substantially in the amounts they contain of the 610 and 630 base pair (bp) families, and in the distribution over the chromosomes of the 480 bp family. The structure of the repeating unit is also different in many members of the 480 bp family in S. montanum. — (2) The substantial differences between species in the amounts of the most highly repeated DNA sequences exist in the absence of any such conspicuous differences in most other repeated sequences which were detected as fluorescent bands after restriction enzyme digestion and gel electrophoresis. — (3) Each of the different highly repeated families can exist independently of the other families, though all the families have telomeric sites. Also, in the outbreeding species, heteromorphisms are frequent, and are particularly conspicuous in hybridisation detecting the 480 bp sequence family. — (4) The association of the highly repeated sequences with heterochromatin, discussed in the accompanying paper is generally true for other species in the genus, and the lower amounts of heterochromatin in other Secale species compared to S. cereale are associated with lower amounts of specific families of highly repeated DNA sequences. — (5) Analysis of highly repeated sequence families is likely to provide an easy method of identification of new accessions of Secale.
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Alwine, J.C., Kemp, D.J., Stark, G.R.: Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl paper and hybridisation with DNA probes. Proc. Natl. Acad. Sci. (Wash.) 74, 5350–5354 (1977)
Appels, R., Driscoll, C., Peacock, W.J.: Heterochromatin and highly repeated DNA sequences in rye (Secale cereale). Chromosoma (Berl.) 70, 67–89 (1978)
Bedbrook, J.R., Jones, J., O'Dell, M., Thompson, R., Flavell, R.B.: A molecular description of telomeric heterochromatin in Secale species. Cell 19, 545–560 (1980a)
Bedbrook, J.R., O'Dell, M., Flavell, R.B.: Amplification of rearranged sequences in cereal plants. Nature (Lond.) 288, 133–137 (1980b)
Bennett, M.D.: Meiotic, gametophytic and early endosperm development in Triticale. In Triticale (R. MacIntyre, M. Campbell ed.). Ottawa, Canada: Industrial Development Research Centre, 137–148 (1973)
Bennett, M.D.: Heterochromatin, aberrant endosperm nuclei and grain shrivelling in wheat-rye genotypes. Heredity 39, 411–419 (1977)
Bennett, M.D., Gustafson, J.P., Smith, J.B.: Variation in nuclear DNA in the genus Secale. Chromosoma (Berl.) 61, 149–176 (1977)
Denhardt, D.T.: A membrane filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun. 23, 641–646 (1966)
Flavell, R.B.: Molecular changes in chromosomal DNA organisation and origins of phenotypic variation. Chromosomes Today 7, 42–54 (1980)
Flavell, R.B., Bedbrook, J.R., Jones, J., O'Dell, M., Gerlach, W., Dyer, T.A., Thompson, R.D.: Molecular events in cereal genome evolution. In: The 4th John Innes Symposium (D.R. Davies, D.A. Hopwood eds.), pp 15–30. John Innes Charity (1982)
Flavell, R.B., Rimpau, J., Smith, D.B.: Repeated sequence DNA relationships in four cereal genomes. Chromosoma (Berl.) 63, 205–222 (1977)
Fry, K., Salser, W.: Nucleotide sequences of HS-α satellite DNA from Kangaroo rat Dipodomys ordii and characterization of similar sequences in other rodents. Cell 12, 1069–1084 (1977)
Gill, B., Kimber, G.: The Giemsa C-banded karyotype of rye. Proc. Natl. Acad. Sci. (Wash.) 71, 1247–1249 (1974)
Graham, D.E.: The isolation of high molecular weight DNA from whole organisms or large tissue masses. Anal. Biochem. 85, 609–613 (1978)
Holmquist, G.P., Dancis, B.: Telomere replication, kinetochore organizers and satellite DNA evolution. Proc. Natl. Acad. Sci. (Wash.) 76, 4566–4570 (1979)
Hutchinson, J., Flavell, R.B., Jones, J.: Physical mapping of plant chromosomes by in situ hybridisation. In: Genetic Engineering. Vol. III, (Stetlow, J., Hollaender, A. eds.). New York: Plenum Press (1981)
Jain, S.K.: Cytogenetics of rye (Secale spp). Bibliogr. Genet. 19, 1–86 (1960)
Jones, J.D.G., Flavell, R.B.: The mapping of highly repeated DNA families and their relationship to C-bands in chromosomes of Secale cereale. Chromosoma (Berl.) 86, 595–612 (1982)
Jones, R.N., Rees, H.: The influence of B-chromosomes upon the nuclear phenotypes of rye. Chromosoma (Berl.) 24, 158–176 (1968)
Khush, G.S.: Cytogenetics and evolutionary structure in Secale. II Interrelationships of the wild species. Evolution 16, 484–496 (1962)
Khush, G.S.: Cytogenetic and evolutionary studies in Secale. III Cytogenetics of weedy ryes and origin of cultivated rye. Ecm. Bot. 17 (1), 60–71 (1963a)
Khush, G.S.: Cytogenetic and evolutionary studies in Secale. IV Secale vavilovii and its biosystematic status. Z. Pflanzenzücht. 50, 34–43 (1963b)
Khush, G.S., Stebbins, G.L.: Cytogenetics and evolutionary studies on Secale. I Some new data on the ancestry of S. cereale. Am. J. Bot. 49, 723–730 (1961)
Kobylanskii, U.D.: The systematics and phylogeny of the genus Secale L. Byull Vses ordena Lenina Inst Rastenioboslstva imeni N. Ivavilov p. 48 1975
Kranz, A.T.: Karyotype analyis in meiosis: Giemsa banding in the genus Secale. Theor. App. Genet. 47, 101–107 (1976)
Lima-de-Faria, A.: Chromomere analysis of the chromosome complement of rye. Chromosoma (Berl.) 5, 1–68 (1952)
Maniatis, T., Jeffrey, A., Van de Sande, H.: Chain length determination of small double and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry 14, 3787–3794 (1975a)
Maniatis, T., Jeffrey, A., Kleid, P.G.: Nucleotide sequence of the rightward operator of phage λ Proc. Natl. Acad. Sci. (Wash.) 72, 1184–1188 (1975b)
Merker, A.: The Cytogenetic effect of heterochromatin in hexaploid Triticale. Hereditas (Lund) 83, 215–222 (1976)
Riley, R., Chapman, V.: The production and phenotypes of wheat-rye chromosome addition lines. Heredity 12, 301–305 (1958)
Sarma, N.P., Natarajan, A.T.: Identification of heterochromatic regions in the chromosomes of rye. Hereditas (Lund) 74, 233–238 (1973)
Singh, R.J., Röbbelen, G.: Comparison of somatic Giemsa banding pattern in several species of rye. Z. Pflanzenzücht. 75, 270–285 (1975)
Singh, R.J., Röbbelen, G.: Identification by Giemsa technique of the translocations separating cultivatied rye from three wild species of Secale. Chromosoma (Berl.) 59, 217–225 (1977)
Southern, R.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517 (1975)
Stutz, H.C.: On the origin of cultivated rye. Am. J. Bot. 59, 59–70 (1972)
Sutcliffe, J.G.: pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucl. Acids Res. 5, 2721–2728 (1978)
Thomas, J.B., Kaltsikes, P.J.: A bouquet-like attachment plate for telomeres in leptotene of rye revealed by heterochromatin staining. Heredity 36, 155–162 (1976)
Wahl, G.M., Stern, M., Stutz, G.R.: Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper hybridisation by using dextran sulphate. Proc. Natl. Acad. Sci. (Wash.) 76, 3683–3687 (1979)
Zohary, D.: Origin of South-west Asiatic cereals: wheats, barley, oats and rye. In: Plant Life of South West Asia (P.H. Davis, P.C. Harper, I.C. Hedge, eds.). Edinburgh: Bot. Soc. 1971
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Jones, J.D.G., Flavell, R.B. The structure, amount and chromosomal localisation of defined repeated DNA sequences in species of the genus Secale . Chromosoma 86, 613–641 (1982). https://doi.org/10.1007/BF00285607
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DOI: https://doi.org/10.1007/BF00285607