Abstract
In the males of the olive scale insect, Parlatoria oleae (2n=8), the paternal set of chromosomes becomes heterochromatic during late cleavage or early blastula and remains so until spermatogenesis. Immediately before the onset of meiosis in the males one or more heterochromatic chromosomes disappear from each primary spermatocyte. At prophase four euchromatic and from one to three heterochromatic chromosomes are present in each cell. The disappearance of the heterochromatic chromosomes before meiosis could be due either to the dehetero-chromatization of the heterochromatic chromosomes and their subsequent pairing with their euchromatic homologues, or to the destruction of the heterochromatic chromosomes. — The alternative interpretations of spermatogenesis in P. oleae were tested by using chromosome aberrations, which had been induced in the heterochromatic set by paternal X-irradiation, as genetic markers in breeding tests of about 400 X1 males. Meiosis was examined in X1 males which showed conspicuous chromosomal rearrangements in their somatic cells. The absence of either heteromorphic chromosome pairs or multivalents at spermatogenesis and the failure of the X1 males to transmit any form of chromosome aberration induced by paternal irradiation is strong evidence that the heterochromatic chromosomes are destroyed in P. oleae. — The evolutionary relationships of the chromosome systems in the coccids are considered. Models are outlined for the derivation of a Comstockiella system involving chromosome destruction either from a lecanoid sequence or from a hypothetical Comstockiella sequence involving chromosome pairing. Problems concerning the control of chromosome destruction are discussed.
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Beardsley, J. W., Brown, S. W.: The biology of the coccids. New York: Appleton-Century-Crofts (in preparation).
Bennett, F. D., Brown, S. W.: Life history and sex determination in the diaspine scale, Pseudaulacaspis pentagona (Targ.) (Coccoidea). Canad. Ent. 90, 317–325 (1958).
Berlowitz, L.: Correlation of genetic activity, heterochromatization, and RNA metabolism. Proc. nat. Acad. Sci. (Wash.) 53, 68–73 (1965).
Bradley, M. V.: An acetocarmine squash technic for mature embryo sacs. Stain Technol. 23, 29–40 (1948).
Brown, S. W.: Chromosome behavior in Comstockiella sabilis (Comstk.) (Coccoidea-Diaspididae). Genetics 42, 362–363 (1957).
—, Spontaneous chromosome fragmentation in the armored scale insects (Coccoidea-Diaspididae). J. Morph. 106, 159–185 (1960a).
Brown, S. W.: Chromosome aberrations in two aspidiotine species of the armored scale insects (Coccoidea-Diaspididae). Nucleus (Calcutta) 3, 135–160 (1960b).
—, The Comstockiella system of chromosome behavior in the armored scale insects (Coccoidea: Diaspididae). Chromosoma (Berl.) 14, 360–406 (1963).
—, Automatic frequency response in the evolution of male haploidy and other coccid chromosome systems. Genetics 49, 797–817 (1964).
—, Chromosomal survey of the armored and palm scale insects (Coccoidea: Diaspididae and Phoenicococcidae). Hilgardia (Berkeley) 36, 189–294 (1965).
—, Chromosome systems in the Eriococcidae (Coccoidea-Homoptera). I. A survey of several genera. Chromosoma (Berl.) 22, 126–150 (1967).
—, Developmental control of heterochromatization in coccids. Genetics, Suppl. 61, 191–198 (1969).
—, Bennett, F. D.: On sex determination in the diaspine scale Pseudaulacaspis pentagona (Targ.) (Coccoidea). Genetics 42, 510–523 (1957).
—, Cleveland, C.: Meiosis in the male of Puto albicans (Coccoidea-Homoptera). Chromosoma (Berl.) 24, 210–232 (1968).
—, Nelson-Rees, W. A.: Radiation analysis of a lecanoid genetic system. Genetics 46, 983–1007 (1961).
—, Nur, U.: Heterochromatic chromosomes in the coccids. Science 145, 130–136 (1964).
Chandra, H. S.: Cytogenetic studies following high dosage paternal irradiation in the mealybug, Planococcus citri. I. Cytology of X1 embryos. Chromosoma (Berl.) 14, 310–329 (1963).
Hughes-Schrader, S.: Cytology of coccids (Coccoidea-Homoptera). Advanc. Genet. 2, 127–203 (1948).
Hughes-Schrader, S., Ris, H.: The diffuse spindle attachment of coccids, verified by the mitotic behavior of induced chromosome fragments. J. exp. Zool. 87, 429–456 (1941).
Kitchin, R. M.: A radiation analysis of a Comstockiella chromosome system (Homoptera: Coccoidea). Ph. D. dissertation, University of California, Berkeley (1969).
- Heterochromatin in the Comstockiella system. In: The biology of the coccids. (Beardsley, J. W., and S. W. Brown, eds.). New York: Appleton-Century-Crofts, (in preparation).
McClintock, B.: Controlling elements and the gene. Cold. Spr. Harb. Symp. quant. Biol. 21, 197–216 (1956).
Nelson-Rees, W. A.: The effects of radiation-damaged heterochromatic chromosomes on male fertility in the mealybug, Planococcus citri (Risso). Genetics 47, 661–683 (1962).
Nur, U.: A modified Comstockiella chromosome system in the olive scale insect, Parlatoria oleae (Coccoidea: Diaspididae). Chromosoma (Berl.) 17, 104–120 (1965).
—, Nonreplication of heterochromatic chromosomes in a mealy bug, Planococcus citri (Coccoidea: Homoptera). Chromosoma (Berl.) 19, 439–448 (1966).
—, Chromosome systems in the Eriococcidae (Coccoidea-Homoptera). II. Gossyparia spuria and Eriococcus araucariae. Chromosoma (Berl.). 22, 151–163 (1967a).
—, Reversal of heterochromatization and the activity of the paternal chromosome set in the male mealybug. Genetics 56, 375–389 (1967b).
Robison, W. G.: Microtubules in relation to the motility of a sperm syncytium in an armored scale insect. Ph. D. dissertation, University of California, Berkeley (1965).
Schrader, F.: The chromosomes of Pseudococcus nipae. Biol. Bull. (Woods Hole) 40, 279–302 (1921).
—, Hughes-Schrader, S.: Haploidy in metazoa. Quart. Rev. Biol. 6, 411–438 (1931)
Snow, R.: Hydrochloric acid-carmine as a stain for chromosomes in squash preparations. Stain Technol. 38, 9–13 (1963).
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Dedicated to Dr. Sally Hughes-Schrader on the occasion of her seventy-fifth birthday.
From a dissertation submitted in partial fulfillment of the requirements of Doctor of Philosophy in Genetics.
This work was supported by grant GB 8196 from the National Science Foundation to Dr. Spencer W. Brown, and by a National Institutes of Health Fellowship 1 F02 CA 44173-01 to the author from the National Cancer Institute.
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Kitchin, R.M. A radiation analysis of a comstockiella chromosome system: destruction of heterochromatic chromosomes during spermatogenesis in Parlatoria oleae (Coccoidea: Diaspididae). Chromosoma 31, 165–197 (1970). https://doi.org/10.1007/BF00285146
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DOI: https://doi.org/10.1007/BF00285146