Abstract
We have analyzed the role of RNA editing in the correction of mismatched base pairs in tRNA secondary structures in mitochondria of the flowering plant Oenothera berteriana. Comparison of genomic and cDNA sequences from unprocessed primary transcripts of the newly characterized genes for tRNACys, tRNAAsn and tRNATle and the previously described gene for tRNAPhe revealed single nucleotide discrepancies in the tRNACys and tRNAPhe sequences. While the change in the anticodon stem of tRNACys alters a C-T to a T-T mismatch, the nucleotide transition in the tRNAPhe restores a conventional T-A Watson-Crick base pair, replacing a C-A mismatch in the acceptor stem. Since both nucleotide alterations are conversions from genomic cytidines to thymidines in the cDNA (uridines in the tRNAs), they are attributed to RNA editing, which is observed in nearly all mRNAs from plant mitochondria.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Beier H, Lee MC, Sekiya T, Kuchino Y, Nishimura S (1992) Two nucleotides next to the anticodon of cytoplasmic rat tRNAAsp are likely generated by RNA editing. Nucleic Acids Res 20:2679–2683
Benne R (1992) RNA editing in trypanosomes. Mol Biol Rep 16:217–227
Benne R, Van den Burg J, Brakenhoff J, Sloof P, Van Boom JH, Tromp MC (1986) Major transcript of the frarneshift coxII from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA. Cell 46:819–826
Binder S, Brennicke A (1993a) Transcription initiation sites in mitochondria of Oenothera berteriana. J Biol Chem 268:7849–7855
Binder S, Brennicke A (1993b) A tRNA gene transcription initiation site is similar to mRNA and rRNA promoters in plant mitochondria. Nucleic Acids Res 21:5012–5019
Binder S, Schuster W, Grienenberger J-M, Weil JH, Brennicke A (1990) Genes for tRNAGly, tRNAHis, tRNALys, tRNAPhe, tRNASer and tRNATyr are encoded in Oenothera mitochondrial DNA. Curr Genet 17:353–358
Binder S, Marchfelder A, Brennicke A, Wissinger B (1992) RNA editing in trans-splicing intron sequences of nad2 mRNAs in Oenothera mitochondria. J Biol Chem 267:7615–7623
Bird S, Duncker B, Garber P, Bonen L (1989) Nucleotide sequence of the bean mitochondrial DNA region containing the tRNAAsn and tRNATyr genes. Nucleic Acids Res 17:4379
Bonnard G, Gualberto JM, Lamattina L, Grienenberger JM (1992) RNA editing in plant mitochondria. Crit Rev Plant Sci 10:503–524
Cattaneo R (1991) Different types of messenger RNA editing. Annu Rev Genet 25:71–88
Gualberto JM, Domon C, Weil J-H, Grienenberger J-M (1989) Nucleotide sequence of the wheat mitochondrial tRNAGlu (UUC) gene. Nucleic Acids Res 17:3586
Izuchi S, Sugita M (1989) Nucleotide sequence of a tomato mitochondrial tRNACys (GCA) gene. Nucleic Acids Res 17:1248
Izuchi S, Terachi T, Sakamoto M, Mikami T, Sugita M (1990) Structure and expression of tomato mitochondrial genes coding for tRNACys (GCA), tRNACys (GUU) and tRNATyr (GUA): a native tRNACys gene is present in dicot plants but absent in monocot plants. Curr Genet 18:239–243
Janke A, Pääbo S (1993) Editing of a tRNA anticodon in marsupial mitochondria changes its codon recognition. Nucleic Acids Res 21:1523–1525
Joyce PBM, Gray MW (1989) Aspartate and asparagine tRNA genes in wheat mitochondrial DNA: a cautionary note on the isolation of tRNA genes from plants. Nucleic Acids Res 17:7865–7878
Joyce PBM, Spencer DF, Bonen L, Gray MW (1988) Genes for tRNAAsp, tRNAPro, tRNATyr and two tRNAsSer in wheat mitochondrial DNA. Plant Mol Biol 10:251–262
Lonergan KM, Gray MW (1993) Editing of transfer RNAs in Acanthamoeba castellanii mitochondria. Science 259:812–816
Maréchal-Drouard L, Ramamonjisoa D, Cosset A, Weil J-H, Dietrich A (1993) Editing corrects mispairing in the acceptor stem of bean and potato mitochondrial phenylalanine transfer RNAs. Nucleic Acids Res 21:4909–4914
Miller DL, Ling M, Wang S, Yang N, Costandy H (1993) RNA editing in mitochondria of Physarum polycephalum. In: Brennicke A, Kück U (eds) Plant mitochondria, VCH Chemie, Weinheim and New York, pp 53–61
Oda K, Yamato K, Ohta E, Nakamura Y, Takemura M, Nozato N, Akashi K, Kanegae T, Ogura Y, Kohchi T, Ohyama K (1992) Gene organization deduced from the complete sequence of liverwort Marchantia polyrnorpha mitochondrial DNA. J Mol Biol 223:1–7
Parks TD, Dougherty WG, Levings CS III, Timothy DH (1984) Identification of two methionine transfer RNA genes in the maize mitochondrial genome. Plant Physiol 76:1079–1082
Saks ME, Sampson JR, Abelson JN (1994) The transfer RNA identity problem: a search for rules. Science 263:191–197
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Habor, New York
Schulman LH (1991) Recognition of tRNAs by aminoacyl-tRNA synthetases. Prog Nucleic Acid Res Mol Biol 41:23–87
Schuster W, Brennicke A (1991) RNA editing makes mistakes in plant mitochondria: editing loses sense in transcripts of a rps19 pseudogene and in creating stop codons in coxI and rps3 mRNA of Oenothera. Nucleic Acids Res 19:6923–6928
Schuster W, Hiesel R, Wissinger B, Schobel W, Brennicke A (1988) Isolation and analysis of plant mitochondria and their genomes. In: Shaw C (ed) Plant molecular biology. A practical approach. IRL Press, Oxford, pp 79–102
Schuster W, Ternes R, Knoop V, Hiesel R, Wissinger B, Brennicke A (1991a) Distribution of RNA editing sites in Oenothera mitochondrial mRNAs and rRNAs. Curr Genet 20:397–404
Schuster W, Wissinger B, Hiesel R, Unseld M, Gerold E, Knoop V, Marchfelder A, Binder S, Schobel W, Scheike R, Grönger P, Ternes R, Brennicke A (1991b) Between DNA and protein — RNA editing in plant mitochondria. Physiol Plant 81:437–445
Singhal RP, Fallis PAM (1979) Structure, function, and evolution of transfer RNAs. Prog Nucleic Acid Res Mol Biol 23:227–263
Steinberg S, Misch A, Sprinzl M (1993) Compilation of transfer RNA sequences and sequences of transfer RNA genes. Nucleic Acids Res 21:3011–3015
Weber F, Dietrich A, Weil J-H, Maréchal-Drouard L (1990) A potato mitochondrial isoleucine tRNA is coded for by a mitochondrial gene possessing a methionine anticodon. Nucleic Acids Res 18:5027–5030
Wissinger B, Schuster W, Brennicke A (1991) Trans splicing in Oenothera mitochondria: nad1 mRNAs are edited in exon and trans-splicing group 11 intron sequences. Cell 65:473–482
Author information
Authors and Affiliations
Additional information
Communicated by: R. Hagemann
Rights and permissions
About this article
Cite this article
Binder, S., Marchfelder, A. & Brennicke, A. RNA editing of tRNAPhe and tRNACys in mitochondria of Oenothera berteriana is initiated in precursor molecules. Molec. Gen. Genet. 244, 67–74 (1994). https://doi.org/10.1007/BF00280188
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00280188