Summary
A method of cold-shock synchronization of immature granulocytic cells from peripheral blood or bone marrow is described. It is shown that this method provides an increased yield of early metaphases and offers advantages over others currently employed.
The peaks of mitotic activity following the cold-shock treatment differ for patients with acute non-lymphoblastic leukemia (ANLL) and patients with chronic myeloid leukemia (CML) by an interval of 2 h.
This method is considered to be suitable for routine cytogenetic studies on hematological patients.
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Hagemeijer, A., et al.: Improved identification of chromosomes of leukemic cells in methotrexate-treated cultures. Cytogenet. Cell Genet. 23, 208–212 (1979)
Newton, A. A.: Synchronous division of animal cells in culture. In: Synchrony in cell division and growth, Zeuthen, E. (ed.), pp. 441–466. New York: Wiley (Interscience) 1964a
Newton, A. A.: Synchronous division of animal cells in culture. In: Synchrony in cell division and growth, Zeuthen, E. (ed.), pp. 601–604. New York: Wiley (Interscience) 1964b
Nias, A. H. W., Fox, M.: Review Article: Synchronization of mammalian cells with respect to the mitotic cycle. Cell Tissue Kinet. 4, 375–398 (1971)
Rao, P. N., Engelberg, J.: Effects of temperature on the mitotic cycle of normal synchronized mammalian cells. In: Cell synchrony. Studies in biosynthetic regulation, Cameron, I. L., Padilla, G. M. (eds.), pp. 332–352. New York, London: Academic Press 1966
Yunis, J. J.: High resolution of human chromosomes. Science 191, 1268–1270 (1976)
Yunis, J. J., Sawyer, J. R., Ball, D. W.: Characterization of banding patterns of metaphase-prophase G-banded chromosomes and their use in gene mapping. Cytogenet. Cell Genet. 22, 679–683 (1978)
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Boucher, B., Norman, C.S. Cold synchronization for the study of peripheral blood and bone marrow chromosomes in leukemias and other hematologic disease states. Hum Genet 54, 207–211 (1980). https://doi.org/10.1007/BF00278974
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DOI: https://doi.org/10.1007/BF00278974