Summary
Progeny of 66 plants regenerated from callus cultures derived from immature embryos of Norstar winter wheat were evaluated as seedlings for tolerance to controlled freezing. Greater freezing tolerance than the parent cultivar was observed in both R2 and R3 regenerate families. LT50 values (predicted temperatures at which mean survival frequencies are 50%) for four families in the R2 generation and three families in the R3 were significantly lower than that of Norstar. In both R2 and R3 generations, most families did not differ significantly from the cultivar Norstar, by three separate measures of tolerance. Significant variation among families was observed in both R2 and R3 generations for survival, but not for plant height. Variation within family in the R3 generation was also significant, though smaller than that among families. In the R3 generation, eighteen families were significantly less freezing tolerant than Norstar according to LT50, while thirteen were significantly less tolerant according to survival at a minimum temperature of-17 °C.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ahloowalia BS, Sherington J (1985) Transmission of somaclonal variation in wheat. Euphytica 34:525–537
Chen THH, Gusta LV, Fowler DB (1983) Freezing injury and root development in winter cereals. Plant Physiol 73:773–777
Chen THH, Lazar MD, Scoles GJ, Gusta LV, Kartha KK (1987) Somaclonal variation in a population of winter wheat. J Plant Physiol 130:27–36
Dix PJ, Street HE (1976) Selection of plant cell lines with enhanced chilling resistance. Ann Bot 40:903–910
Fowler DB, Gusta LV (1979) Selection for winter hardiness in wheat. 1. Identification of genotypic variability. Crop Sci 19:769–772
Grafius JE (1981) Breeding for winter hardiness. In: Olien CR, Smith MN (eds) Analysis and improvement of plant cold hardiness. CRC Press, Boca Raton, Florida, pp 162–174
Gusta LV, Fowler DB (1979) Cold resistance and injury in winter cereals. In: Mussell H, Staples R (eds) Stress physiology in crop plants. Wiley and Sons, New York, pp 160–177
Larkin PF (1985) In vitro culture and cereal breeding. In: Bright SWJ, Jones MGK (eds) Cereal tissue and cell culture. Nijhoff/Junk Publishers, Dordrecht, The Netherlands, pp 273–296
Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214
Larkin PF, Ryan SA, Brettel RIS, Scowcroft WR (1984) Heritable somaclonal variation in wheat. Theor Appl Genet 67:443–455
Limin AE, Fowler DB (1981) Cold hardiness of some relatives of hexaploid wheat. Can J Bot 59:572–573
Limin AE, Fowler DB (1982) The expression of cold hardiness in Triticum species amphidiploids. Can J Genet Cytol 24:51–56
Limin AE, Fowler DB (1983) Genetics of cold hardiness. In: Fowler DB, Gusta LV, Slinkard AE, Hobin BA (eds) New frontiers in winter wheat production. University of Saskatchewan, Saskatoon, Canada, pp 193–207
Maddock SE, Lancaster VA, Risiott R, Franklin J (1983) Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat (Triticum aestivum). J Exp Bot 34:915–926
Nabors MW, Gibbs SE, Bernstein CS, Meis ME (1980) NaCl-tolerant tobacco plants from cultured cells. Z Pflanzenphysiol 97:13–17
Ojima K, Ohira A (1983) Characterization of aluminum and manganese tolerant cell lines selected from carrot cell cultures. Plant Cell Physiol 24:789–797
Sears RG, Deckard EL (1982) Tissue culture variability in wheat: callus induction and regeneration. Crop Sci 22:546–550
Templeton-Somers KM, Sharp WR, Pfister RM (1981) Selection of cold-resistant cell lines of carrot. Z Pflanzenphysiol 103:139–148
Yurkova GN, Levenko BA, Novoshilov (1982) Plant regeneration in wheat tissue culture. Biochem Physiol Pflanz 177:337–344
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
NRCC No. 28387. This publication describes research performed as part of the program of the Plant Biotechnology Institute of the National Research Council of Canada through Plant Biotechnology Fund contract No. 31964-4-0021 awarded to T. H. H. Chen and M. D. Lazar, Alberta Research Council, Edmonton, Canada
Rights and permissions
About this article
Cite this article
Lazar, M.D., Chen, T.H.H., Gusta, L.V. et al. Somaclonal variation for freezing tolerance in a population derived from norstar winter wheat. Theoret. Appl. Genetics 75, 480–484 (1988). https://doi.org/10.1007/BF00276753
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00276753