Abstract
Callus tissues from different explants (hypocotyl, cotyledon, root, leaf and fruit) of Solanum eleagnifolium Cav. were cultured on a modified Murashige-Skoog medium, with 1 mg.1−1 2,4-D as the sole growth regulator. The presence of the alkaloid solasodine was determined by spectrophotometric and TLC methods. Its concentration ranged from 1.00 to 2.15 mg.g−1 DW. The calli from different explants showed a direct association between the solasodine production and their growth, although they have a different production rate. It was also observed that about the seventh week of culture the metabolite concentration decreased in all cases.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- DW:
-
dry weight
- TLC:
-
thin layer chromatography
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Communicated by J.M. Widholm
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Nigra, H.M., Caso, O.H. & Giulietti, A.M. Production of solasodine by calli from different parts of Solanum eleganifolium Cav. plants. Plant Cell Reports 6, 135–137 (1987). https://doi.org/10.1007/BF00276671
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DOI: https://doi.org/10.1007/BF00276671