Abstract
The correlation between manganese deficiency, loss of mitochondrial respiratory chain NADH: ubiquinone oxidoreductase (complex I) activity and citric acid overproduction in the Aspergillus niger strain B 60 was analysed. With increasing manganese-supplementation of the production medium the loss of complex I activity and the production of citric acid was reduced. Addition of manganese during growth stopped further loss of complex I activity and further increase of citric acid production. A possible causality between complex I deficiency and citric acid overproduction is discussed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Archibald F (1986) Manganese: its aquisition by and function in the lactic acid bacteria. CRC Crit Rev Microbiol 13: 63–109
Daum G (1985) Lipids of mitochondria. Biochim Biophys Acta 822: 1–42
Garraway MO, Evans RC (1984) Fungal nutrition and physiology. Wiley, New York, pp 151–154
Hartl F, Neupert W (1990) Protein sorting to mitochondria: evolutionary conservations of folding and assembly. Science 247: 930–938
Hatefi Y (1985) The mitochondrial electron transport and oxidative phosphorylation system. Annu Rev Biochem 54: 1019–1069
Hockertz S, Plönzig J, Auling G (1987) Impairment of DNA formation is an early event in Aspergillus niger under manganese starvation. Appl Microbiol Biotechnol 25: 590–593
Kubicek CP, Röhr M (1977) Influence of manganese on enzyme synthesis and citric acid accumulation in Aspergillus niger. Eur J Appl Microbiol 4: 167–175
Kubicek CP, Röhr M (1986) Citric acid fermentation. CRC Crit Rev Biotechnol 3: 331–373
Lambers H (1982) Cyanide-resistant respiration: a non-phosphorylating electron transport pathway acting as an energy overflow. Physiol Plant 55: 478–485
Ma H, Kubicek CP, Röhr M (1985) Metabolic effects of manganese deficiency in Aspergillus niger: evidence for increased protein degradation. Arch Microbiol 141: 266–268
Mandal SB, Sen PC, Chakrabarti P, Sen K (1978) Effect of respiratory deficiency and temperature on the mitochondrial lipid metabolism of Aspergillus niger. Can J Microbiol 24: 586–592
Mikolajczyk S, Brody S (1990) De novo fatty acid synthesis mediated by acyl-carrier protein in Neurospora crassa. Eur J Biochem 187: 431–437
Runswick MJ, Fearnley IM, Skehel JM, Walker JE (1991) Presence of an acyl carrier protein in NADH: ubiquinone oxidoreductase from bovine heart mitochondria. FEBS Lett 286: 121–124
Sackmann U, Zensen R, Röhlen D, Jahnke U, Weiss H (1991) The acyl-carrier protein in Neurospora crassa mitcohondria is a subunit of NADH: ubiquinone reductase (complex I). Eur J Biochem 200: 463–469
Schmidt M, Wallrath J, Dörner A, Weiss H (1992) Disturbed assembly of the respiratory chain NADH: ubiquinone reductase (complex I) in citric-acid-accumulating Aspergillus niger strain B 60. Appl Microbiol Biotechnol 36: 667–672
Wallrath J, Schmidt M, Weiss H (1991) Concomitant loss of respiratory chain NADH: ubiquinone reductase (complex I) and citric acid accumulation in Aspergillus niger. Appl Microbiol Biotechnol 36: 76–81
Weiss H, Friedrich T, Hofhaus G, Preis D (1991) The respiratory chain NADH dehydrogenase (complex I) of mitochondria. Eur J Biochem 197: 563–576
Williams RJP (1982) Free manganese(II) and iron (II) cations can act as intracellular cell controls. FEBS Lett 140: 3–10
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wallrath, J., Schmidt, M. & Weiss, H. Correlation between manganese-deficiency, loss of respiratory chain complex I activity and citric acid production in Aspergillus niger . Arch. Microbiol. 158, 435–438 (1992). https://doi.org/10.1007/BF00276305
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00276305