Summary
The distribution and frequency of aphidicolin-induced common fragile sites were studied in chromosomes of cultured skin fibroblasts and PHA-stimulated lymphocytes from five normal individuals; 0.2 μM aphidicolin was added for the last 26 h of culture. Skin fibroblasts from five fra(X)-positive patients were also studied in the same manner. Fragile sites most frequently found in fibroblasts from normal individuals were 3q26.2, 7q11.23, 16q23, 1p31, 10q11.2, 12q23 and 7q31, whereas those in lymphocytes from the same individuals were 3p14, 16q23, Xp22, 7q32 and 14q24. The distribution of fragile sites in fibroblasts from fra(X)-positive patients was essentially identical with that in normal individuals. The average number of gaps and breaks in 100 metaphases was 36.8 in fibroblasts from normal individuals, 113.8 in those from fra(X)-positive patients, and 279 in lymphocytes from normal individuals. Their rates of chromosome-type breaks and gaps were 7.9%, 29.7% and 54.5%, respectively. Thus, the distribution and frequency of aphidicolin-induced fragile sites were different between skin fibroblasts and lymphocytes, possibly reflecting differences in their DNA replication sequence or gene activity.
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Glover TW, Berger C, Coyle J, Echo B (1984) DNA polymerase alpha inhibition by aphidicolin induces gaps and breaks at common fragile sites in human chromosomes. Hum Genet 67:136–142
Kuwano A, Kajii T (1987) Synergistic effect of aphidicolin and ethanol on the induction of common fragile sites. Hum Genet 75:75–78
Kuwano A, Sugio Y, Murano I, Kajii T (1988) Common fragile sites induced by folate deprivation, BrdU and aphidicolin: their frequency and distribution in Japanese individuals. Jpn J Hum Genet 33:355–364
Morgan R, Morgan SS, Hecht BK, Hecht F (1988) Fragile sites at 4q23 and 7q11.23 unique to bone marrow cells. Cancer Genet Cell Genet 31:47–54
Sutherland GR, Baker E (1986) Induction of fragile sites in fibroblasts. Am J Hum Genet 38:573–575
Sutherland GR, Mattei JF (1987) Report of the committee on cytogenetic markes. (9th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 46:316–324
Yunis JJ, Soreng AL, Bowe AE (1987) Fragile sites are targets of diverse mutagens and carcinogens. Oncogene 1:59–69
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Murano, I., Kuwano, A. & Kajii, T. Fibroblast-specific common fragile sites induced by aphidicolin. Hum Genet 83, 45–48 (1989). https://doi.org/10.1007/BF00274145
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DOI: https://doi.org/10.1007/BF00274145