Summary
The fatty acid synthetase (FAS) gene FAS1 of the alkane-utilizing yeast Yarrowia lipolytica was cloned and sequenced. The gene is represented by an intron-free reading frame of 6228 by encoding a protein of 2076 amino acids and 229980 Da molecular weight. This protein exhibits a 58% sequence similarity to the corresponding Saccharomyces cerevisiae FAS β-subunit. The sequential order of the five FAS1-encoded enzyme domains, acetyl transferase, enoyl reductase, dehydratase and malonyl/palmityl-transferase, is co-linear in both organisms. This finding agrees with available evidence that the functional organization of FAS genes is similar in related organisms but differs considerably between unrelated species. In addition, previously reported conflicting data concerning the 3′ end of S. cerevisiae FAS1 were re-examined by genomic and cDNA sequencing of the relevant portion of the gene. Thereby, the translational stop codon was shown to lie considerably downstream of both published termination sites. The S. cerevisiae FAS1 gene thus has a corrected length of 6153 by and encodes a protein of 2051 amino acids and 228667 Da molecular weight.
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Amy CM, Witkowski A, Naggert J, Williams B, Rhandawa Z, Smith S (1989) Molecular cloning and sequencing of cDNAs encoding the entire rat fatty acid synthase. Proc Natl Acad Sci USA 86:3114–3118
Beck J, Ripka S, Siegner A, Schiltz E, Schweizer E (1990) The multifunctional 6-methylsalicylic acid synthase gene of Penicillium patulum: its gene structure relative to that of other polyketide synthases. Eur J Biochem 192:487–498
Chirala SS, Kuziora MA, Spector DM, Wakil SJ (1987) Complementation of mutations and nucleotide sequence of FAS1 gene encoding β subunit of yeast fatty acid synthetase. J Biol Chem 262:4231–4240
Kawaguchi A, Okuda S (1977) Fatty acid synthetase from Brevibacterium ammoniagenes: formation of monounsaturated fatty acids by a multienzyme complex. Proe Natl Acad Sci USA 74:3180–3183
Langford DJ, Klintz FJ, Donath C, Gallwitz D (1984) Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast. Cell 36:645–653
Lynen F (1980) On the structure of fatty acid synthetase of yeast. Eur J Biochem 112:431–442
Mohamed AH, Chirala SS, Mody NH, Huang WY, Wakil SJ (1988) Primary structure of the multifunctional α subunit protein of yeast fatty acid synthetase derived from FAS2 gene sequence. J Biol Chem 263:12315–12325
Schweizer E, Werkmeister K, Jain MK (1978) Fatty acid biosynthesis in yeast. Mol Cell Biochem 21:95–106
Schweizer E, Müller G, Roberts LM, Schweizer M, Rösch J, Wiesner P, Beck J, Stratmann D, Zauner I (1987) Genetic control of fatty acid synthetase biosynthesis and structure in lower fungi. Fat Sci Technol 89:570–577
Schweizer M, Roberts LM, Höltke HJ, Takabayashi K, Höllerer E, Hoffmann B, Müller G, Köttig H, Schweizer E (1986) The pentafunctional FAS1 gene of yeast: its nucleotide sequence and order of the catalytic domains. Mol Gen Genet 203:479–486
Schweizer M, Takabayashi K, Laux T, Beck KF, Schreglmann R (1989) Rat mammary gland fatty acid synthase: localization of the constituent domains and two functional polyadenlyation/ termination signals in the cDNA. Nucleic Acids Res 17:567–586
Wakil SJ, Stoops JK, Joshi VC (1983) Fatty acid synthesis and its regulation. Annu Rev Biochem 52:537–579
Wiesner P, Beck J, Beck KF, Ripka S, Müller G, Lücke S, Schweizer E (1988) Isolation and sequence analysis of the fatty acid synthetase FAS2 gene from Penicillium patulum. Eur J Biochem 177:69–79
Yuan Z, Lin W, Hammes GG (1988) Molecular cloning and sequencing of DNA complementary to chicken liver fatty acid synthase mRNA. Proc Natl Acad Si USA 85:6328–6331
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Communicated by C.P. Hollenberg
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Köttig, H., Rottner, G., Beck, KF. et al. The pentafunctional FAS1 genes of Saccharomyces cerevisiae and Yarrowia lipolytica are co-linear and considerably longer than previously estimated. Mol Gen Genet 226, 310–314 (1991). https://doi.org/10.1007/BF00273618
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DOI: https://doi.org/10.1007/BF00273618