Abstract
Whole plant regeneration via somatic embryogenesis was obtained in pea (Pisum sativum L.) using explants from immature embryos or shoot apex segments. The induction of somatic embryos required picloram or 2,4-D. Germination of fully-developed embryos was accomplished by subculture on medium with only cytokinin and then on medium supplemented with cytokinins in combination with a reduced auxin concentration. Plantlets obtained from both zygotic embryos and shoot apices were transferred to soil and were grown to maturity. Nine plants were examined cytologically, revealing three tetraploids (2n=4x=28) and six diploids (2n=2x=14).
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Abbreviations
- Picloram:
-
4-amino-3,5,6-trichloropicolinic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphthaleneacetic acid
- BA:
-
6-benzylaminopurine
- IBA:
-
indole-3-butyric acid
References
Allavena A, Rossetti L (1983) Acta Hort. 131:239–246
Atanassov A, Mehandjiev A (1979) C.R. Acad. Bulg. Sci. 32:115–118
Barwale UB, Kerns HR, Widholm JM (1986) Planta 167:473–481
Beversdorf WD, Bingham ET (1977) Crop Sci. 17:307–311
Bhargava S, Chandra N (1983) Plant Cell Rep. 2:47–50
Devreux M (1970) Pisum Newslet. 2:12
Eskew DL, Welch R M, Cary E A (1983) Science 222:621–622
Ezhova TA, Bagrova AM, Gostimiskii SA (1985) Sov. Plant Physiol., Russian original 32:409–414
Gamborg OL, Constabel F, Shyluk JP (1974) Physiol. Plant. 30:125–128
Gamborg OL, Davis BP, Stahlhut RW (1983) Plant Cell Rep. 2:213–215
Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50:151–158
Hussey G, Gunn HV (1984) Plant Sci. Let. 37:143–148
Jacobsen H-J, Kysely W (1984) Plant Cell Tis. Org. Cult. 3:319–324
Jacobsen H-J, Ingensiep HW, Herlt M, Kaul MLH (1980) In: Plant Cell Cultures: Results and Perspectives (Sala F, Parisi B, Cella R, Ciferri O, eds.) Elsevier/North Holland Biomedical Press pp 319–324
Kunakh VA, Alkhimova EG, Voitjuk LI, Alpatova LK (1984) In: Proceed. Intnl. Symp. Olomouc, Czechoslovakia (Novac F J, Havel L, Dolezel J, eds.) Inst. of Exp. Botany Czechoslovak Academy of Science, Prage p 135.
Kysely W (1985) Pisum Newslet. 17:38–39
Lazzeri PA, Hildebrand DF, Collins GB (1986) Plant Mol. Biol. Rep. 3:160–167
Lazzeri PA, Hildebrand DF, Collins GB (1987) Plant Cell Tis. Org. Cult. (In Press)
Lippmann B, Lippmann G (1984) Plant Cell Rep. 3:215–218
Lönnig W-E (1982) Theor. Appl. Genet. 63:55–264
Malmberg RL (1979) Planta 146:243–244
Martins LS, Sondahl MR (1984) J. Plant Physiol. 117:97–103
Mroginski LA, Kartha KK (1981) Plant Cell Rep. 1:64–66
Mroginski LA, Kartha KK (1984) Plant Breed. Rev. 2:236–237
Murashige T, Skoog P (1962) Physiol. Plant. 15:473–497
Phillips GC, Collins GB (1981) Plant Cell Tis. Org. Cult. 1:123–129
Phillips GC, Collins GB (1985) In: Handbook of Plant Cell Culture (Sharp WR, Evans DA, Ammirato PV, Yamada Y, eds.) Macmillan, Pub. Co., N.Y. pp 169–210
Ranch J, Oglesby L, Zielinski AC (1985) In Vitro 21:653–658
Rubluo A, Kartha KK, Mroginski LA, Dyck J (1984) J. Plant Physiol. 117:119–130
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Communicated by O.L. Gamborg
KAES Journal Article No. 87-3-4
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Kysely, W., Myers, J.R., Lazzeri, P.A. et al. Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.). Plant Cell Reports 6, 305–308 (1987). https://doi.org/10.1007/BF00272005
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DOI: https://doi.org/10.1007/BF00272005