Abstract
We compared the binding of Agrobacterium tumefaciens by freshly isolated root cap cells with susceptibility of plants to crown gall tumorigenesis. A high binding reaction was strongly correlated with susceptibility to tumorigenesis in a survey of the binding of strain B6 to cells from 48 species in 17 families. In reciprocal experiments with nine virulent A. tumefaciens strains, tumors developed in plant-bacteria combinations that gave a high binding response in the root cap cell assay. Binding was quantified by direct measurement of the number of bacteria bound to the periphery of individual cells. Root cap cells from six susceptible species bound significantly more bacteria than did cells from five resistant species.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
DeCleene M (1985) Phytopath. Z. 113:81–89
Douglas CJ, Halperin W, Gordon M, Nester E (1985a) J. Bacteriol. 161:764–766
Douglas CJ, Staneloni RJ, Rubin R, Nester E (1985b) J. Bacteriol. 161:850–860
Hawes MC, Pueppke SG (1986) Am. J. Bot. 73:1466–1473
Hernalsteens JP, Thia-Teong L, Schell J, Van Montagu, M (1984) EMBO J. 3:3039–3041
Hooykaas-Van Slogteren GMS, Hooykaas PJJ, Schilperoort RA (1984) Nature 311:763–764
Lippincott JA, Lippincott BB (1980) In: Bacterial Adherence, Receptors and Recognition, Ser. B, Vol. 6, EH Beachey, (ed) Chapman and Hall, London, pp. 375–398
Neff NT, Binns AN (1985) Plant Physiol. 77-35–42
Nester EW, Gordon MP, Amasino RM, Yanofsky MF (1984) Ann. Rev. Plant Physiol. 35:387–413
Otten,LABM, Schilperoort, RA (1978) Biochim. Biophys. Acta 527:497–500
Pueppke SG (1984) In: Plant Microbe Interactions. Kosuge T, Nester EW, (eds). John Wiley and Sons. NY. pp. 215–216
Pueppke SG, Benny UK (1981) Physiol. Plant Pathol. 18:169–179
Author information
Authors and Affiliations
Additional information
Communicated by J.M. Widholm
Rights and permissions
About this article
Cite this article
Hawes, M.C., Pueppke, S.G. Correlation between binding of Agrobacterium tumefaciens by root cap cells and susceptibility of plants to crown gall. Plant Cell Reports 6, 287–290 (1987). https://doi.org/10.1007/BF00272000
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00272000