Summary
Labelled chloroplast rRNAs from Spinacia oleracea were hybridized to restriction endonuclease digests of chloroplast DNA from Oenothera hookeri and Euglena gracilis, to mitochondrial DNA of Acanthamoeba castellanii, and to DNA of the E. coli rrn B operon in the transducing phage lambda rifd18. The degree of homology is greatest for the 16S rRNA gene. Greater than 90% occurs between the two higher plant genes, 80% homology to the lower plant gene, 60%–70% homology to the bacterial gene, and 20% homology to the mitochondrial gene. The degree of hybridization varied considerably for the 23S and the 5S rRNA genes. Very high homology exists between the two higher plant genes, only about 50% homology for both the Euglena and bacterial genes, and no significant homology for the mitochondrial genes. These results show that any chloroplast (or E. coli) rRNA may be used as a probe to identify rRNA genes in other ctDNAs.
Two RNA populations, each enriched for a different ctDNA-encoded mRNA, proved useful in the location of these genes on both higher plant ctDNAs. No significant hybridization was obtained using these probes to the Euglena ctDNA which seems to be too distantly related.
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Abbreviations
- Md:
-
megadalton, 106 dalton
- bp, kbp:
-
base pair, kilo base pair
- SSC:
-
Standard saline citrate, 1 times SSC is 0.15M sodium, chloride, 0.015 M trisodium citrate, pH, 6.8
- mtDNA:
-
mitochondrial DNA
- ctDNA:
-
chloroplast DNA
- ctrRNA:
-
chloroplast ribosomal RNA
References
Bedbrook JR, Link G, Coen DM, Bogorad L, Rich A (1978) Maize plastid gene expressed during photoregulated development. Proc Natl Acad Sci USA 75:3060–3064
Bedbrook JR, Kolodner R (1979) The structure of chloroplast DNA. Annu Rev Plant Physiol 30:593–620
Bohnert HJ (1972) Thesis, Universität Heidelberg
Bohnert HJ, Driesel AJ, Herrmann RG (1976) Characterization of the RNA compounds synthesized by isolated unbroken chloroplasts. In: Bücher Th, Neupert W, Sebald W, Werner S (eds) Genetics and biogenesis of chloroplasts and mitochondria. Elsevier-North Holland. Amsterdam, p 629
Bohnert HJ, Driesel AJ, Crouse EJ, Gordon K, Herrmann RG, Steinmetz A, Mubumbila M, Keller M, Burkard G, Weil JH (1979) Presence of a transfer RNA gene in the spacer sequence between the 16S and 23S rRNA genes of spinach chloroplast DNA. FEBS Lett 103:52–56
Borst P, Grivell LA (1971) Mitochondrial ribosomes. FEBS Lett. 13:73–88
Brosius J, Palmer ML, Kennedy PJ, Noller HF (1979) Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 75:4801–4805
Carbon P, Ehresmann C, Ehresmann B, Ebel JP (1979) The complete nucleotide sequence of the ribosomal 16S RNA from Escherichia coli. Eur J Biochem 100:399–410
Coen DM, Bedbrook JR, Bogorad L, Rich A (1977) Maize chloroplast DNA fragment encoding the large subunit of ribulosebisphosphate carboxylase. Proc Natl Acad Sci USA 74:5487–5491
Commerford SL (1971) Iodination of nucleic acids in vitro. Biochemistry 10:1993–1999
Cronse EJ, Schmitt JM, Bohnert HJ, Gordon K, Driesel AJ, Herrmann RG (1978) Intramolecular compositional heterogeneity of Spinacia and Euglena chloroplast DNAs. In: Akoyunoglou G, Argyroudi-Akoyunoglou J (eds) Chloroplast development. Elsevier-North Holland, Amsterdam, p 565
Crouse EJ, Vandrey JP, Stutz E (1974) Hybridization studies with RNA and DNA isolated from Euglena gracilis chloroplasts and mitochondria. FEBS Lett 42:262–266
Driesel AJ (1979) Thesis, Universität Düsseldorf
Driesel AJ, Speirs J, Bohnert HJ (1980) Spinach chloroplast in RNA for a 32 Kd polypeptide-size and localization on the physical map of the chloroplast DNA. Biochim Biophys Acta (in press)
Driesel AJ, Crouse EJ, Gordon K, Bohnert HJ, Herrmann RG, Steinmetz A, Mubumbila M, Keller M, Burkard G, Weil JH (1979) Fractionation and identification of spinach chloroplast transfer RNAs and mapping of their genes on the restriction map of chloroplast DNA. Gene 6:285–306
Gordon KHJ, Crouse EJ, Bohnert HJ, Herrmann RG (1980) Restriction endonuclease cleavage site map of chloroplast DNA from Oenothera plastome IV. Theoret appl Genet (in press)
Hallick RB, Rushlow KE, Orozco EM, Stiegler GL, Gray PW (1979) Chloroplast DNA of Euglena gracilis: gene mapping and selective in vitro transcription of the ribosomal RNA region. In: ICU-UGLA Symposium on Molecular Biology, vol 15 Academic Press, New York
Herrmann RG, Possingham JV (1980) Plastid DNA — the Plastome. In: Reinert J (ed) Results and problems in cell differentiation: The Chloroplast. Springer, Berlin Heidelberg New York (in press)
Keller M (1979) Thesis, Université Louis Pasteur de Strasbourg
Koncz Cs, Sain B (1980) Homology between rRNA of Escherichia coli and mitochondrial DNA of maize. FEBS Lett. 109:141–144
Jenni B, Stutz E (1979) Analysis of Euglena gracilis chloroplast DNA. Mapping of a DNA sequence complementary to 16S rRNA outside of the three rRNA gene sets. FEBS Lett 102:95–98
Kopecka H, Crouse EJ, Stutz E (1977) The Euglena gracilis chloroplast genome: analysis by restriction enzymes. Eur J Biochem 72:525–535
Leaver CJ, Harmey MA (1976) Higher-plant mitochondrial ribosomes contain a 5S ribosomal ribonucleic acid component. Biochem J 157:275–277
Loening UE (1968) Molecular weights of ribosomal RNA in relation to evolution. J Mol Biol 38:355–365
Lund E, Dahlberg JE, Lindahl L, Jaskunas SR, Dennis PP, Nomura M (1976) Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of Escherichia coli. Cell 7:165–178
Malnoe P, Rochaix JD, Chua NH, Spahr PF (1979) Characterization of the gene and messenger DNA of the large subunit of ribulose 1,5-diphosphate carboxylase in Chlamydomonas reinhardii. J Mol Biol 133:417–434
Mubumbila M, Burkard G, Keller M, Steinmetz A, Crouse E, Weil JH (1980) Hybridization of bean, spinach, maize and Euglena chloroplast transfer RNAs with homologous and heterologous chloroplast DNA: an approach to the study of homology between chloroplast tRNAs from various species. Biochim Biophys Acta (in press)
Nomura M, Morgan EA, Jaskunas SR (1977) Genetics of bacterial ribosomes. Annu Rev Genet 11:297–347
Payne PI, Dyer TA (1971) Characterization of cytoplasmic and chloroplast 5S ribosomal ribonucleic acid from broad-bean leaves. Biochem J 124:83–89
Rawson JRY, Kushner SR, Vapnek D, Alton NK, Boerma CL (1978) Chloroplast ribosomal RNA genes in Euglena gracilis exist as three clustered tandem repeats. Gene 3:191–209
Rossi L, Gualerzi C (1970) Non-random differences in the base composition of chloroplast and cytoplasmic ribosomal RNA from some higher plants. Life Sci 9:1401–1407
Schwartz RM, Dayhoff MO (1978) Origins of prokaryotes, eukaryotes, mitochondria and chloroplasts. Science (Wash.) 199:395–403
Schwarz Zs, Kössel H (1979) Sequencing of the 3′terminal region of a 16S rRNA gene from Zea mays chloroplasts reveals homology with E. coli 16S rRNA. Nature 279:520–523
Schwarz Zs, Kössel H (1980) The Primary Structure of 16S rDNA from Zea mays Chloroplast is homologous to E. coli 16S rDNA. Nature 283:739–742
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Stanley WM, Bock RM (1965) Isolation and physical properties of the ribosomal ribonucleic acid of Escherichia coli. Biochemistry 4:1302–1311
Whitfeld P (1977) Chloroplast RNA. In: Stewart PR, Letham DS (eds) The ribonucleic acids. Springer Verlag, New York, p 297
Whitfeld PR, Leaver CJ, Bottomley W, Atchison BA (1977) A new species of chloroplast RNA. In: Bogorad L, Weil JH (eds) Acides nucleiques et synthèse des proteines chez les Végétaux. CNRS, Paris, p 235
Whitfeld PR, Herrmann RG, Bottomley W (1978) Mapping of the ribosomal RNA genes on spinach chloroplast DNA. Nucl Acids Res 5:1741–1751
Wollgiehn R, Parthier B (1979) RNA synthesis in isolated chloroplasts of Euglena gracilis. Plant Sci Lett 16:203–210
Young R, Macklis R, Steitz J (1979) Sequence of the 16S–23S spacer region in two ribosomal operons of E. coli. J Biol Chem 254:3264–3271
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Communicated by E. Bautz
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Bohnert, H.J., Gordon, K.H.J. & Crouse, E.J. Homologies among ribosomal RNA and messenger RNA genes in chloroplasts, mitochondria and E. coli . Molec. Gen. Genet. 179, 539–545 (1980). https://doi.org/10.1007/BF00271743
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DOI: https://doi.org/10.1007/BF00271743