Summary
Bacillus amyloliquefaciens N produces two restriction enzymes, BamNI and BamNx. Subtilis-phage ϕ1 is strongly restricted by BamNx. We isolated ϕ1 rH, a mutant of phage ϕ1, which overcame the BamNx-restriction by producing inhibitor. This inhibitor inactivated BamNx specifically and reversibly. The inhibitor directly interacted with BamNx and the inactivation might be the result of formation of a binary complex. The inhibitory activity was sensitive to treatment with trypsin. The molecular weight of the inhibitor protein was estimated to be approximately 20,000 daltons by gel filtration.
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Makino, O., Saito, H. & Ando, T. Bacillus subtilis-phage φ1 overcomes host-controlled restriction by producing BamNx inhibitor protein. Molec. Gen. Genet. 179, 463–468 (1980). https://doi.org/10.1007/BF00271735
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DOI: https://doi.org/10.1007/BF00271735