Summary
Thirty-six thermophilic archaebacteria and nine extremely thermophilic eubacteria have been screened on solid media for extracellular amylase, protease, hemicellulase (xylanase), cellulase, pectinase and lipase activities. Extracellular enzymes were detected in 14 archaebacteria belonging to three different orders. Twelve of these were able to degrade starch and casein and the two Thermofilum strains were able to degrade starch, xylan and carboxymethylcellulose. Three of the eubacteria could degrade only starch. The other six (including four Thermotoga strains) all had activity against starch, xylan and carboxymethylcellulose, and one also had activity against casein. Some of the amylolytic archaebacteria released α-glucosidase, β-glucosidase, amylase and transglucosylase activities into liquid media containing starch or maltose. Thermotoga strain FjSS3B.1 released amylase, xylanase, cellulase and β-glucosidase activities into the medium when grown in the presence of substrates. When the partially purified enzymes from Thermotoga and some of the archaebacteria were compared with known thermostable enzymes the majority were found to be the most thermostable of their type. The β-glucosidase, xylanase and cellulase from Thermotoga and two α-glucosidases, a β-glucosidase, an amylase and a pullulanase from archaebacteria all have half-lives of at least 15 min at 105°C.
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References
Brock TD (1985) Life at high temperatures. Science 230:132–138
Brock TD, Brock KM, Belly RT, Weiss RL (1972) Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature. Arch Microbiol 84:54–68
Chi CL, Casida LE Jr (1984) Gelrite as a gelling agent in media for the growth of thermophilic microorganisms. Appl Environ Microbiol 47:427–429
Cowan DA, Daniel RM (1982) Purification and some properties of an extracellular protease (caldolysin) from an extreme thermophile. Biochim Biophys Acta 705:293–305
Cowan DA, Smolenski KA, Daniel RM, Morgan HW (1987) An extremely thermostable extracellular proteinase from a strain of the archaebacterium Desulfurococcus growing at 88°C. Biochem J 247:121–133
Daniel RM, Cowan DA, Morgan HW, Curran MP (1982) A correlation between protein thermostability and resistance to proteolysis. Biochem J 207:641–644
Daniel RM, Morgan HW, Martin AM (1986) The industrial potential of extreme thermophiles. Ind Biotechnol 6:89–91
Fujita SC, Oshima T, Imahori K (1976) Purification and properties of d-glyceraldehyde-3-phosphate dehydrogenase from an extreme thermophile, Thermus thermophilus strain HB8. Eur J Biochem 64:57–68
Hagerman AE, Blau DM, McClure AL (1985) Plate assay for determining the time of production of protease, cellulase, and pectinases by germinating fungal spores. Anal Biochem 151:334–342
Hopkins RH, Bird R (1954) The action of some alpha-amylases on amylose. Biochem J 56:86–99
Huber R, Langworthy TA, König H, Thomm M, Woese CR, Sleytr UB, Stetter KO (1986) Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90°C. Arch Microbiol 144:324–333
Huser BA, Patel BKC, Daniel RM, Morgan HW (1986) Isolation and characterisation of a novel extremely thermophilic, anaerobic, chemo-organotrophic eubacterium. FEMS Microbiol Lett 37:121–127
Jasperse-Herst PM (1984) Isolation and characterization of extremely thermophilic archaebacteria from New Zealand hot pools. M. Sc. Thesis, University of Waikato, Hamilton, New Zealand
Langworthy TA, Rapporteur, Brock TD, Castenholz RW, Esser AF, Johnson EJ, Oshima T, Tsuboi M, Zeikus JG, Zuber H (1979) Life at high temperatures. Group report. In: Shilo M (ed) Strategies of microbial life in extreme environments. Verlag Chemie, New York, pp 489–502
Lever M (1973) Colorimetric and fluorometric carbohydrate determination with p-hydroxybenzoic acid hydrazide. Biochem Med 7:274–281
Patchett ML, Daniel RM, Morgan HW (1987) Purification and properties of a stable β-glucosidase from an extremely thermophilic anaerobic bacterium. Biochem J 243:779–787
Patel BKC, Morgan HW, Daniel RM (1985) Fervidobacterium nodosum gen. nov. and spec. nov., a new chemoorganotrophic, caldoactive anaerobic bacterium. Arch Microbiol 141:63–69
Patel BKC, Jasperse-Herst PM, Morgan HW, Daniel RM (1986) Isolation of anaerobic, extremely thermophilic, sulphur metabolising archaebacteria from New Zealand hot springs. NZ J Mar Freshwater Res 20:439–445
Pfueller SL, Elliot WH (1969) The extracellular α-amylase of Bacillus stearothermophilus. J Biol Chem 244:48–54
Plant AR, Patel BKC, Morgan HW, Daniel RM (1987) Starch degradation by thermophilic anaerobic bacteria. Syst Appl Microbiol 9:158–162
Reynolds PHS, Sissons CH, Daniel RM, Morgan HW (1986) Comparison of cellulolytic activities in Clostridium thermocellum and three thermophilic, cellulolytic anaerobes. Appl Environ Microbiol 51:12–17
Schofield LR, Neal TL, Patchett ML, Strange RC, Daniel RM, Morgan HW (1988) The purification of cellulase and hemicellulase components from an extreme thermophile by the cloning of enzymes into E. coli. Proceedings of the IXth Enzyme Engineering Conference, Santa Barbara, USA. Ann NY Acad Sci 542:240–243
Singleton R, Amelunxen RE (1973) Proteins from thermophilic microorganisms. Bacteriol Rev 37:320–342
Sonnleitner B, Fiechter A (1983) Advantages of using thermophiles in biotechnological processes: expectations and reality. Trends Biotechnol 1:74–80
Stetter KO, König H, Stackebrandt E (1983) Pyrodictium gen. nov., a new genus of submarine disc-shaped sulphur reducing archaebacteria growing optimally at 105°C. Syst Appl Microbiol 4:535–551
Teather RM, Wood PJ (1982) Use of congo-red polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43:777–780
Wolin EA, Wolin MJ, Wolfe RS (1963) Formation of methane by bacterial extracts. J Biol Chem 238:2882–2886
Zaks A, Klibanov AM (1984) Enzymatic catalysis in organic media at 100°C. Science 224:1249–1251
Zeikus JG (1979) Thermophilic bacteria: ecology, physiology and technology. Enzyme Microb Technol 1:243–252
Zillig W, Holz I, Klenk HP, Trent J, Wunderl S, Janekovic D, Imsel E, Haas B (1987) Pyrococcus woesei, sp. nov., an ultra-thermophilic marine archaebacterium, representing a novel order, Thermococcales. Syst Appl Microbiol 9:62–70
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Bragger, J.M., Daniel, R.M., Coolbear, T. et al. Very stable enzymes from extremely thermophilic archaebacteria and eubacteria. Appl Microbiol Biotechnol 31, 556–561 (1989). https://doi.org/10.1007/BF00270794
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DOI: https://doi.org/10.1007/BF00270794