Summary
Nocardia corynebacteroides SM1 synthesized di- and pentasaccharide lipids when grown on n-alkanes, especially under nitrogen limitation. Optimum conditions for their formation were pH 6.4–6.8, NaNO3 as nitrogen source and yeast extract supplementation of the nutrient. A study of the time course of whole glycolipid production was carried out in a 20-l bioreactor. After extraction of the culture broth with organic solvents three main components could be isolated. Both 1H and 13C nuclear magnetic resonance spectroscopic and elemental analysis studies led to the identification of one novel pentasaccharide lipid and two trehalose corynomycolates. The oligosaccharide lipid showed significant surface and interfacial active properties.
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References
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This paper is dedicated to Prof. Dr. Fritz Wagner, Technical University of Braunschweig, on the occasion of his 60th birthday
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Powalla, M., Lang, S. & Wray, V. Penta- and disaccharide lipid formation by Nocardia corynebacteroides grown on n-alkanes. Appl Microbiol Biotechnol 31, 473–479 (1989). https://doi.org/10.1007/BF00270779
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DOI: https://doi.org/10.1007/BF00270779